Mutational analysis of interaction between coat protein and helper component‐proteinase of<i>Soybean mosaic virus</i>involved in aphid transmission

Seo, Jang‐Kyun; Kang, Seong-Gil; Seo, Bo Yoon; Jung, Jin Kyo; Kim, Kook‐Hyung

doi:10.1111/j.1364-3703.2009.00603.x

Cited by 49 publications

(36 citation statements)

References 48 publications

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“…Even though in vivo interaction between HCPro and CP from PPV has not been observed by bimolecular fluorescence complementation and yeast two-hybrid assays (reference 58 and our unpublished results), there are numerous reports showing that potyviral HCPro and CP from several viruses are able to interact in diverse systems (8,11,57,(64)(65)(66)(67). Thus, it is reasonable to think that direct interaction between HCPro and CP is required for the enhancement of viral particle yield.…”

Section: Discussionmentioning

confidence: 77%

A Novel Role of the Potyviral Helper Component Proteinase Contributes To Enhance the Yield of Viral Particles

Vallí

Gallo

Calvo

et al. 2014

J Virol

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The helper component proteinase (HCPro) is an indispensable, multifunctional protein of members of the genus Potyvirus and other viruses of the family Potyviridae. This viral factor is directly involved in diverse steps of viral infection, such as aphid transmission, polyprotein processing, and suppression of host antiviral RNA silencing. In this paper, we show that although a chimeric virus based on the potyvirus Plum pox virus lacking HCPro, which was replaced by a heterologous silencing suppressor, caused an efficient infection in Nicotiana benthamiana plants, its viral progeny had very reduced infectivity. Making use of different approaches, here, we provide direct evidence of a previously unknown function of HCPro in which the viral factor enhances the stability of its cognate capsid protein (CP), positively affecting the yield of virions and consequently improving the infectivity of the viral progeny. Site-directed mutagenesis revealed that the ability of HCPro to stabilize CP and enhance the yield of infectious viral particles is not linked to any of its previously known activities and helped us to delimit the region of HCPro involved in this function in the central region of the protein. Moreover, the function is highly specific and cannot be fulfilled by the HCPro of a heterologous potyvirus. The importance of this novel requirement in regulating the sorting of the viral genome to be subjected to replication, translation, and encapsidation, thus contributing to the synchronization of these viral processes, is discussed. IMPORTANCEPotyviruses form one of the most numerous groups of plant viruses and are a major cause of crop loss worldwide. It is well known that these pathogens make use of virus-derived multitasking proteins, as well as dedicated host factors, to successfully infect their hosts. Here, we describe a novel requirement for the proper yield and infectivity of potyviral progeny. In this case, such a function is performed by the extensively studied viral factor HCPro, which seems to use an unknown mechanism that is not linked to its previously described activities. To our knowledge, this is the first time that a factor different from capsid protein (CP) has been shown to be directly involved in the yield of potyviral particles. Based on the data presented here, we hypothesize that this capacity of HCPro might be involved in the coordination of mutually exclusive activities of the viral genome by controlling correct assembly of CP in stable virions.

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Section: Discussionmentioning

confidence: 77%

A Novel Role of the Potyviral Helper Component Proteinase Contributes To Enhance the Yield of Viral Particles

Vallí

Gallo

Calvo

et al. 2014

J Virol

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“…Seo et al (2010) revealed strong interaction between SMV CP and HC-Pro that depended on several crucial amino acid residues on CP and on HC-Pro.…”

Section: Cps and Vector Transmissionmentioning

confidence: 96%

Multiple functions of capsid proteins in (+) stranded RNA viruses during plant–virus interactions

Weber

Bujarski

2015

Virus Research

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“…The amino acid motifs PTK and KITC interact with the viral CP motif DAG and the internal surface of aphid stilets, respectively, creating a molecular bridge and mediating non-persistent virus transmission (Seo et al, 2010). HC-pro also contains a cystein protease domain (C6 family) acting on its own C terminus (Urcuqui-Inchima et al, 2001;Adams et al, 2005).…”

Section: Expression Strategy and Non-structural Proteinsmentioning

confidence: 99%

Unfolding the secrets of plum pox virus: from epidemiology to genomics

Šubr¹,

Glasa²

2013

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Summary. -Over an approximately 80-year period since the description of the Sharka disease, the plum pox virus (PPV) has been thoroughly studied on various levels of the infection cycle. World-wide distribution of the virus, severity of the disease for the fruit industry with a potential to be further increased and discovery/ emergence of new strains make PPV the most epidemiologically important viral pathogen of stone fruit trees. The history of PPV research reflects the development of analytical methods applicable in plant virology. In particular the establishment of molecular biology with reverse genetics and improvement of DNA sequencing technology have further contributed to the increase in knowledge on PPV variability, evolution, replication and interaction with host plants. This review gives a comprehensive summary of PPV data accumulated progressively, from the biological characterization of the disease to recent attempts aimed at using the PPV-based vectors for expression of exogenous proteins in plants.

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Mutational analysis of interaction between coat protein and helper component‐proteinase ofSoybean mosaic virusinvolved in aphid transmission

Cited by 49 publications

References 48 publications

A Novel Role of the Potyviral Helper Component Proteinase Contributes To Enhance the Yield of Viral Particles

A Novel Role of the Potyviral Helper Component Proteinase Contributes To Enhance the Yield of Viral Particles

Multiple functions of capsid proteins in (+) stranded RNA viruses during plant–virus interactions

Unfolding the secrets of plum pox virus: from epidemiology to genomics

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