Mutations in a Cyclic Nucleotide–Gated Channel Lead to Abnormal Thermosensation and Chemosensation in C. elegans

Komatsu, Hidetoshi; Mori, Ikue; Rhee, Jeong−Seop; Akaike, Norio; Ohshima, Yasumi

doi:10.1016/s0896-6273(00)80202-0

Cited by 399 publications

(432 citation statements)

References 66 publications

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“…However, the reciprocal is not true; expression of tax-2 does not ameliorate the tax-4 phenotype. Consistent with the results of the genetic rescue experiments, Tax-4 by itself forms a functional ion channel when expressed in HEK293 cells in vitro, whereas Tax-2 does not [10,11]. Tax-4 is highly sensitive to cGMP versus cAMP (K D values of 4× 10 −7 versus 1.4×10 −4 M, respectively [10]).…”

Section: Cyclic-nucleotide Mediated Transduction Of Thermal Stimuli Isupporting

confidence: 82%

“…Consistent with the results of the genetic rescue experiments, Tax-4 by itself forms a functional ion channel when expressed in HEK293 cells in vitro, whereas Tax-2 does not [10,11]. Tax-4 is highly sensitive to cGMP versus cAMP (K D values of 4× 10 −7 versus 1.4×10 −4 M, respectively [10]). When co-expressed, Tax-4 and Tax-2 form a functional heteromeric channel that is ~25-fold less sensitive to cGMP in comparison to Tax-4 alone but still retains selectivity for cGMP over cAMP [11].…”

Section: Cyclic-nucleotide Mediated Transduction Of Thermal Stimuli Isupporting

confidence: 82%

“…The tax-4 and tax-2 genes encode α-and β-subunits, respectively, of a cGMP-gated ion channel that is most similar to those expressed in rod photoreceptors. In addition to AFD neurons, Tax-2 and Tax-4 proteins are also expressed in other sensory neurons including ASE, AWB, and AWC and are localized to cilia and dendritic structures of these neurons [9,10]. Mutants are also defective in many aspects of olfaction and chemosensation of salts, consistent with this neuronal localization.…”

Section: Cyclic-nucleotide Mediated Transduction Of Thermal Stimuli Isupporting

confidence: 58%

“…Genetic studies suggest that thermal signals are transduced downstream of an unknown "thermosensor" in these neurons through a cGMP-gated ion channel encoded by the tax-2 and tax-4 genes [9,10]. Mutants in both tax-2 and tax-4 exhibit identical thermotactic deficits that are akin to those observed in AFD-ablated animals.…”

Section: Cyclic-nucleotide Mediated Transduction Of Thermal Stimuli Imentioning

confidence: 99%

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Temperature sensing across species

McKemy

2007

Pflugers Arch - Eur J Physiol

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The ability to detect changes in temperature is a fundamental sensory mechanism for every species and provides organisms with a detailed view of the environment. This review focuses on what is known of the neuronal and molecular substrates for thermosensation across species, focusing on the three robust model systems extensively used to study sensory signaling, the nematode Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and the laboratory mouse. Nematodes migrate to thermal climes that are amenable to their survival, a behavior that is regulated primarily through a single sensory neuron. Additionally, nematodes "learn" to seek out this temperate zone based upon their prior experience, a robust model of learning and memory. Drosophila larvae also prefer select thermal zones that are optimal for growth and have also developed vigorous mechanisms to avoid unfavorable conditions. In mammals, the transduction mechanisms for thermosensation have been identified primarily due to the fact that naturally occurring plant products evoke distinct psychophysical sensation of temperature change. More remarkably, the elucidation of the molecular sensors in mammals, along with those in Drosophila, has demonstrated conservation in the molecular mediators of temperature sensation across diverse species.

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Section: Cyclic-nucleotide Mediated Transduction Of Thermal Stimuli Isupporting

confidence: 82%

Section: Cyclic-nucleotide Mediated Transduction Of Thermal Stimuli Isupporting

confidence: 82%

Section: Cyclic-nucleotide Mediated Transduction Of Thermal Stimuli Isupporting

confidence: 58%

Section: Cyclic-nucleotide Mediated Transduction Of Thermal Stimuli Imentioning

confidence: 99%

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Temperature sensing across species

McKemy

2007

Pflugers Arch - Eur J Physiol

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“…In the AWC olfactory neurons and in several other neuron types, G proteins may activate guanylyl cyclases, resulting in gating of the TAX-2/4 cGMP-gated channels [20,22,[141][142][143] (Fig. 2a).…”

Section: The Molecules For Taste and Smellmentioning

confidence: 99%

Generation and modulation of chemosensory behaviors in C. elegans

Sengupta

2007

Pflugers Arch - Eur J Physiol

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C. elegans recognizes and discriminates among hundreds of chemical cues using a relatively compact chemosensory nervous system. Chemosensory behaviors are also modulated by prior experience and contextual cues. Because of the facile genetics and genomics possible in this organism, C. elegans provides an excellent system in which to explore the generation of chemosensory behaviors from the level of a single gene to the motor output. This review summarizes the current knowledge on the molecular and neuronal substrates of chemosensory behaviors and chemosensory behavioral plasticity in C. elegans.

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G proteins, chemosensory perception, and the C. elegans genome project: An attractive story

Wilkie

1999

Bioessays

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Heterotrimeric G proteins, consisting of α, β, and γ subunits, couple ligand‐bound seven transmembrane domain receptors to the regulation of effector proteins and production of intracellular second messengers. G protein signaling mediates the perception of environmental cues in all higher eukaryotic organisms, including yeast, Dictyostelium, plants, and animals. The nematode Caenorhabditis elegans is the first animal to have complete descriptions of its cellular anatomy, cell lineage, neuronal wiring diagram, and genomic sequence. In a recent paper, Jansen et al.(1) used sequence searches of the C. elegans genome database to identify all heterotrimeric G protein genes (20 Gα, 2 Gβ, 2 Gγ). C. elegans encodes one ortholog of each of the four Gα classes found in metazoans and 16 new Gα genes. The orthologous genes are widely expressed, whereas 14 of the divergent Gα genes are almost exclusively expressed in sensory neurons where they may regulate perception and chemotaxis.BioEssays 21:713–717, 1999. © 1999 John Wiley & Sons, Inc.

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Mutations in a Cyclic Nucleotide–Gated Channel Lead to Abnormal Thermosensation and Chemosensation in C. elegans

Cited by 399 publications

References 66 publications

Temperature sensing across species

Temperature sensing across species

Generation and modulation of chemosensory behaviors in C. elegans

G proteins, chemosensory perception, and the C. elegans genome project: An attractive story

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