In this work, diffusion couples of bcc phase Ti-Nb-V have been prepared and annealed at different temperatures such as 1273, 1373, and 1473 K. The concentration profile of each diffusion couple was measured with the help of electron microprobe analysis. In addition, the interdiffusion coefficients were obtained from the concentration profiles of diffusion couples, using numerical inverse and Whittle-Green methods. Combining with the thermodynamic database, the atomic mobilities of Ti, Nb and V in bcc Ti-Nb-V alloys were simulated by DICTRA package. In summary, our simulated atomic mobilities have a nice correlation with the experimental data and can reasonably describe the diffusion phenomena, such as diffusion paths and the concentration profiles of the bcc Ti-Nb-V ternary system.