Mutvei's solution: An ideal agent for resolving microgrowth structures of biogenic carbonates

Schöne, Bernd R.; Dunca, Elena; Fiebig, Jens; Pfeiffer, Miriam

doi:10.1016/j.palaeo.2005.03.054

Cited by 222 publications

(158 citation statements)

References 50 publications

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“…Images of 256 by 256 pixels were obtained by rastering the primary beam across a pre-sputtered surface with a pixel dwelling time of 10 ms. Following NanoSIMS imaging, the remaining gold coating was removed and the samples lightly etched in Mutvei's solution following described procedures (Schöne et al, 2005). Similar effects were also obtained by ca.…”

Section: Methodsmentioning

confidence: 84%

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

Gorzelak

Stolarski

Dúbois

et al. 2011

Journal of Structural Biology

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26Mg labeling NanoSIMS SEM a b s t r a c t This paper reports the results of the first dynamic labeling experiment with regenerating spines of sea urchins Paracentrotus lividus using the stable isotope 26 Mg and NanoSIMS high-resolution isotopic imaging, which provide a direct information about the growth process. Growing spines were labeled twice (for 72 and 24 h, respectively) by increasing the abundance of 26 Mg in seawater. The incorporation of 26 Mg into the growing spines was subsequently imaged with the NanoSIMS ion microprobe. Stereom trabeculae initially grow as conical micro-spines, which form within less than 1 day. These micro-spines fuse together by lateral outgrowths and form a thin, open meshwork (inner stereom), which is subsequently reinforced by addition of layered thickening deposits (outer stereom). The (longitudinal) growth rate of the inner stereom is ca. 125 lm/day. A single (ca. 1 lm) thickening layer in the stereom trabeculae is deposited during 24 h. The thickening process is contemporaneous with the formation micro-spines and involves both longitudinal trabeculae and transverse bridges to a similar degree. Furthermore, the skeleton-forming cells remain active in the previously formed open stereom for at least 10 days, and do not migrate upwards until the end of the thickening process. The experimental capability presented here provides a new way to obtain detailed information about the skeleton formation of a multitude of marine, calcite producing organisms.

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Section: Methodsmentioning

confidence: 84%

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

Gorzelak

Stolarski

Dúbois

et al. 2011

Journal of Structural Biology

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“…Cross sections were then glued on glass slides (Crystalbond 509 mounting adhesive), manually ground for 3 min on a 1200-grit size abrasive disk, more finely ground using a 2500-grit size abrasive disk on an automated polishing machine , and finally polished with a diamond suspension (Struers DiaPro Dur 3 μm). In order to better resolve annual growth lines and increments, cross sections were cleaned with ethanol before being etched in the Mutvei's solution for 25 min at 38.5°C (see Schöne et al 2005 for a detailed description of the method). They were then gently rinsed with ultra-pure water and air-dried.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic responses of the endangered freshwater mussel Margaritifera margaritifera to trace metal contamination in the Dronne River, France

Bertucci

Pierron

Thébault

et al. 2017

Environ Sci Pollut Res

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The freshwater pearl mussel Margaritifera margaritifera is one of the most threatened freshwater bivalves worldwide. In this study, we aimed (i) to study the processes by which water quality might affect freshwater mussels in situ and (ii) to provide insights into the ecotoxicological significance of water pollution to natural populations in order to provide necessary information to enhance conservation strategies. M. margaritifera specimens were sampled in two close sites located upstream or downstream from an illegal dumping site. The renal transcriptome of these animals was assembled and gene transcription determined by RNA-seq. Correlations between transcription levels of each single transcript and the bioaccumulation of nine trace metals, age (estimated by sclerochronology), and condition index were determined in order to identify genes likely to respond to a specific factor. Amongst the studied metals, Cr, Zn, Cd, and Ni were the main factors correlated with transcription levels, with effects on translation, apoptosis, immune response, response to stimulus, and transport pathways.However, the main factor explaining changes in gene transcription appeared to be the age of individuals with a negative correlation with the transcription of retrotransposon-related genes. To investigate this effect further, mussels were classified into three age classes. In young, middle-aged and old animals, transcription levels were mainly explained by Cu, Zn and age, respectively. This suggests differences in the molecular responses of this species to metals during its lifetime that must be better assessed in future ecotoxicology studies.

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“…Consequently, an etching technique was used to reveal the shell growth pattern. The etching was accomplished following the procedure described by Aubert et al (2009), which uses a slightly modified Mutvei's solution (Schö ne et al 2005) for 45 min. Here, an increment was defined as the amount of shell between each ridge revealed by the etching.…”

Section: Methodsmentioning

confidence: 99%

In situ giant clam growth rate behavior in relation to temperature: A one‐year coupled study of high‐frequency noninvasive valvometry and sclerochronology

Schwartzmann

Durrieu

Sow

et al. 2011

Limnology & Oceanography

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The life history of 15 giant clams, Hippopus hippopus, was studied in situ in the southern lagoon of New Caledonia; growth rate and animal behavior were studied both by sclerochronology and high-frequency noninvasive (HFNI) valvometry. Electrodes glued on each valve of each specimen recorded the shell-gaping behavior at 0.6-Hz frequency. A nonparametric regression model was used to model clam behavior. The daily increment thickness in the inner layer of five representative clams was measured. H. hippopus has its valves open during the day and partly closed during the night all year round, and shell growth is continuous. The cumulative growth using both techniques was similar, as was the mean daily thickness increment. The occurrence of one increment per day in H. hippopus shell was measured by valvometry. The five sclerochronological profiles were highly similar. Shell growth was significantly correlated to rising sea surface temperature (SST), up to 27uC. At the solar maximum, gaping behavior and increment thickness became erratic. SST-and solar irradiance-related stress could be related to physiological oxidative stress triggered by zooxanthellae symbionts. In the present context of globally increasing SST, our data indicate that the giant clams H. hippopus could live beyond their thermal comfort limits in summer in New Caledonia.

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Mutvei's solution: An ideal agent for resolving microgrowth structures of biogenic carbonates

Cited by 222 publications

References 50 publications

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

Transcriptomic responses of the endangered freshwater mussel Margaritifera margaritifera to trace metal contamination in the Dronne River, France

In situ giant clam growth rate behavior in relation to temperature: A one‐year coupled study of high‐frequency noninvasive valvometry and sclerochronology

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