2019
DOI: 10.3390/vaccines7040182
|View full text |Cite
|
Sign up to set email alerts
|

MVA-Vectored Pentameric Complex (PC) and gB Vaccines Improve Pregnancy Outcome after Guinea Pig CMV Challenge, but Only gB Vaccine Reduces Vertical Transmission

Abstract: (1) Background: A congenital cytomegalovirus (cCMV) vaccine is a major research priority, but the essential glycoprotein target(s) remain unclear. We compared CMV gB (gpgB), gH/gL (gp75/gL), and pentameric complex (gpPC, composed of gH/gL/GP129/GP131/GP133) vaccines in a guinea pig CMV (GPCMV) congenital infection model. (2) Methods: Modified vaccinia virus Ankara (MVA) vaccines expressing GPCMV glycoproteins were used to immunize GPCMV-seronegative, female Hartley guinea pigs (three-dose series, 3 × 107 pfu/d… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
12
0

Year Published

2020
2020
2023
2023

Publication Types

Select...
8
1

Relationship

2
7

Authors

Journals

citations
Cited by 11 publications
(12 citation statements)
references
References 60 publications
0
12
0
Order By: Relevance
“…Though the pentameric complex elicits a high titer of HCMV neutralization activity, the antibodies elicited are protective against HCMV infection of epithelial cells, endothelial cells and monocytes, but not fibroblasts or primary trophoblast progenitor cells [ 46 , 47 , 48 , 49 , 68 , 69 , 70 , 71 ]. It has been suggested that the combination of trimeric gB and pentameric complex proteins may be an optimal prophylactic HCMV vaccine [ 37 , 72 , 73 ]. The effect of gB in combination with pentameric complex has been evaluated using MVA vectored or RNA vaccine that simultaneously express gB and the pentameric complex [ 66 , 67 ].…”
Section: Discussionmentioning
confidence: 99%
“…Though the pentameric complex elicits a high titer of HCMV neutralization activity, the antibodies elicited are protective against HCMV infection of epithelial cells, endothelial cells and monocytes, but not fibroblasts or primary trophoblast progenitor cells [ 46 , 47 , 48 , 49 , 68 , 69 , 70 , 71 ]. It has been suggested that the combination of trimeric gB and pentameric complex proteins may be an optimal prophylactic HCMV vaccine [ 37 , 72 , 73 ]. The effect of gB in combination with pentameric complex has been evaluated using MVA vectored or RNA vaccine that simultaneously express gB and the pentameric complex [ 66 , 67 ].…”
Section: Discussionmentioning
confidence: 99%
“…MVA is a novel and versatile platform for vaccine development against infections for a number of pathogens. While MVA has been used to develop a vaccine against some herpesviruses [ 61 63 ], and especially hCMV [ 29 , 44 , 64 66 ], its use in EEHV vaccinology has not yet been explored. One major advantage of using MVA for vaccine development is the ability to incorporate multiple target antigens into a single MVA vector.…”
Section: Discussionmentioning
confidence: 99%
“…Furthermore, a protective role of HCMV-specific antibodies was reported in animal models [99,100] as well as in transplant recipients [101]. In the guinea pig model, which represents the only small animal model available for quantitative measures of virus transmission and efficacy of vaccination, both MVA-vectored PC and gB vaccines improved pregnancy outcome after a guinea pig CMV challenge, but only gB reduced vertical transmission [102]. On the other hand, inclusion of the guinea pig PC in a vaccine design greatly improved protection against cCMV in the guinea pig model [103].…”
Section: Multiple Strain Hcmv Infection and Hcmv Vaccinementioning
confidence: 98%