Myconoside interacts with the plasma membranes and the actin cytoskeleton and provokes cytotoxicity in human lung adenocarcinoma A549 cells

Kostadinova, Aneliya; Hazarosova, Rusina; Topouzova-Hristova, Tanya; Moyankova, Daniela; Yordanova, Vesela; Veleva, Ralitsa; Nikolova, Biliana; Momchilova, Albena; Djilianov, Dimitar; Staneva, Galya

doi:10.1007/s10863-021-09928-x

Cited by 4 publications

(21 citation statements)

References 46 publications

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“…To our knowledge, the present results are the first report that states the hormetic-like response of natural phenyl glycoside molecules such as myconoside in MDCKII cell line. It is noteworthy that we did not observe a similar hormetic-like response of myconoside at a low dose treatment of a cancer A549 cell line published in our previous study [13]. Instead, the cytotoxic effect was observed at a progressive increase in myconoside concentration.…”

Section: Discussioncontrasting

confidence: 48%

“…MDCKII cells are known to be used as a model for polarized epithelial monolayers to study changes in cell contacts, plasmalemma polarization and epithelial barrier functions. In our recent study, we demonstrated the effect of myconoside on membrane lipid order, F-actin and ZO-1 rearrangement in relation to cell viability of a non-polarized cancer A549 cell line [13]. Comparative analysis of the obtained results between two cell lines, a polarized non-cancer cell line (MDCKII) and a non-polarized cancer one (A549), are presented in the discussion.…”

Section: Introductionmentioning

confidence: 85%

“…Madin-Darby Canine Kidney II (MDCKII) cells were grown in supplemented Dulbecco's Modified Eagle Medium (DMEM) in flasks (25 cm 2 ) at standard conditions as described in [13].…”

Section: Cell Culturementioning

confidence: 99%

“…Fluorescent staining of F-actin and ZO-1 was performed as described in [13]. For indirect immunofluorescence experiments, fluorescein-conjugated rabbit anti-rat IgG (Boehringer Mannheim Biochemicals, Germany) was used.…”

Section: Fluorescent Staining Of F-actin and Zonula Occludens (Zo-1)mentioning

confidence: 99%

“…For indirect immunofluorescence experiments, fluorescein-conjugated rabbit anti-rat IgG (Boehringer Mannheim Biochemicals, Germany) was used. More details of the protocol are given in [13].…”

Section: Fluorescent Staining Of F-actin and Zonula Occludens (Zo-1)mentioning

confidence: 99%

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Myconoside Affects the Viability of Polarized Epithelial MDCKII Cell Line by Interacting with the Plasma Membrane and the Apical Junctional Complexes

et al. 2022

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The phenyl glycoside myconoside, extracted from Balkan endemic Haberlea rhodopensis, has a positive effect on human health, but the exact molecular mechanism of its action is still unknown. The cell membrane and its associated junctional complex are the first targets of exogenous compound action. We aimed to study the effect of myconoside on membrane organization and cytoskeleton components involved in the maintenance of cell polarity in the MDCKII cell line. By fluorescent spectroscopy and microscopy, we found that at low concentrations, myconoside increases the cell viability by enhancing membrane lipid order and adherent junctions. The opposite effect is observed in high myconoside doses. We hypothesized that the cell morphological and physicochemical changes of the analyzed cell compartments are directly related to cell viability and cell apical-basal polarity. Our finding contributes to a better understanding of the beneficial application of phytochemical myconoside in pharmacology and medicine.

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Section: Discussioncontrasting

confidence: 48%

Section: Introductionmentioning

confidence: 85%

“…Madin-Darby Canine Kidney II (MDCKII) cells were grown in supplemented Dulbecco's Modified Eagle Medium (DMEM) in flasks (25 cm 2 ) at standard conditions as described in [13].…”

Section: Cell Culturementioning

confidence: 99%

Section: Fluorescent Staining Of F-actin and Zonula Occludens (Zo-1)mentioning

confidence: 99%

Section: Fluorescent Staining Of F-actin and Zonula Occludens (Zo-1)mentioning

confidence: 99%

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Myconoside Affects the Viability of Polarized Epithelial MDCKII Cell Line by Interacting with the Plasma Membrane and the Apical Junctional Complexes

et al. 2022

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Haberlea rhodopensis Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression

Staneva,

Dimitrova,

Popov

et al. 2023

IJMS

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Ionizing radiation (IR) and reactive oxygen species (ROS)-induced oxidative stress can cause damage to cellular biomolecules, including DNA, proteins, and lipids. These harmful effects can compromise essential cellular functions and significantly raise the risk of metabolic dysfunction, accumulation of harmful mutations, genome instability, cancer, accelerated cellular senescence, and even death. Here, we present an investigation of HeLa cancer cells’ early response to gamma IR (γ-IR) and oxidative stress after preincubation of the cells with natural extracts of the resurrection plant Haberlea rhodopensis. In light of the superior protection offered by plant extracts against radiation and oxidative stress, we investigated the cellular defence mechanisms involved in such protection. Specifically, we sought to evaluate the molecular effects of H. rhodopensis extract (HRE) on cells subjected to genotoxic stress by examining the components of the redox pathway and quantifying the transcription levels of several critical genes associated with DNA repair, cell cycle regulation, and apoptosis. The influence of HRE on genome integrity and the cell cycle was also studied via comet assay and flow cytometry. Our findings demonstrate that HREs can effectively modulate the cellular response to genotoxic and oxidative stress within the first two hours following exposure, thereby reducing the severity of such stress. Furthermore, we observed the specificity of genoprotective HRE doses depending on the source of the applied genotoxic stress.

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Resurrection Plants—A Valuable Source of Natural Bioactive Compounds: From Word-of-Mouth to Scientifically Proven Sustainable Use

Djilianov,

Moyankova,

Mladenov

et al. 2024

Metabolites

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Resurrection plant species are a group of higher plants whose vegetative tissues are able to withstand long periods of almost full desiccation and recover quickly upon rewatering. Apart from being a model system for studying desiccation tolerance, resurrection plant species appear to be a valuable source of metabolites, with various areas of application. A significant number of papers have been published in recent years with respect to the extraction and application of bioactive compounds from higher resurrection plant species in various test systems. Promising results have been obtained with respect to antioxidative and antiaging effects in various test systems, particularly regarding valuable anticancer effects in human cell lines. Here, we review the latest advances in the field and propose potential mechanisms of action of myconoside—a predominant secondary compound in the European members of the Gesneriaceae family. In addition, we shed light on the possibilities for the sustainable use of natural products derived from resurrection plants.

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Myconoside interacts with the plasma membranes and the actin cytoskeleton and provokes cytotoxicity in human lung adenocarcinoma A549 cells

Cited by 4 publications

References 46 publications

Myconoside Affects the Viability of Polarized Epithelial MDCKII Cell Line by Interacting with the Plasma Membrane and the Apical Junctional Complexes

Myconoside Affects the Viability of Polarized Epithelial MDCKII Cell Line by Interacting with the Plasma Membrane and the Apical Junctional Complexes

Haberlea rhodopensis Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression

Resurrection Plants—A Valuable Source of Natural Bioactive Compounds: From Word-of-Mouth to Scientifically Proven Sustainable Use

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