Mycoplasma hafezii sp. nov., isolated from the trachea of a peregrine falcon (Falco peregrinus)

Ziegler, Luisa; Palau-Ribes, Franca Möller; Enderlein, Dirk; Herbst, W.; Schmidt, Li S.; Lierz, Michael

doi:10.1099/ijsem.0.003235

Cited by 12 publications

(4 citation statements)

References 18 publications

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“…Lecis et al (2016) observed clinical symptoms compatible with mycoplasmosis in a positive individual; in the present study, such occurrence was not observed, despite the high frequency of mycoplasma positive birds. The high frequency found in asymptomatic prey corroborates other studies where a high frequency of mycoplasma was observed in birds of prey with mild or inapparent clinical presentation (Lierz et al 2008, Lecis et al 2010, Ziegler et al 2019.…”

Section: Resultssupporting

confidence: 89%

Investigation of Mycoplasma spp. in birds of the Rio de Janeiro Zoo by isolation and PCR

et al. 2020

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Brazil is one of the countries with the most abundant avifauna in the world. The confinement of birds associated with close contact with other animals and humans favor the spread of agents of respiratory diseases. Among them, mycoplasmas can cause asymptomatic or apparent disease that manifests in birds by coughing, sneezing, rales, conjunctivitis, ocular and nasal discharge. Several described mycoplasmas cause disease in birds, especially Mycoplasma gallisepticum(MG) andMycoplasma synoviae(MS). The diagnosis ofMycoplasmaspp. can be done by clinical observation and laboratory analysis. Molecular diagnosis by PCR was boosted by its speed, sensitivity, and low cost of agent isolation techniques that take up to 21 days to complete. This study aimed to verify the occurrence ofMycoplasmaspp. in birds of the Rio de Janeiro Zoo (Rio Zoo), by isolation and PCR. Of the total 635 birds from the Rio Zoo, 81 were studied for detection ofMycoplasmaspp., when taken for routine health assessment exams. These birds belonged to the following orders: Psittaciformes (45), Accipitriformes (18), Galliformes (7), Piciformes (5), Strigiformes (4), Falconiformes (1) and Cariamiformes (1), all individuals already identified by microchip or leg-ring. There was no isolation of mycoplasmas in any of the samples tested, whereas, in the PCR, 62.96% (51/81) were positive, with 1.96% (1/51) identified as MG and 19.61% (10/51) as MS, representing 1.23% (1/81) and 12.34% (10/81) of the total population studied. PCR was shown to be a more effective technique than isolation in the detection ofMycoplasmaspp. in birds. It was possible to detect mycoplasmas in birds from Riozoo with no clinical respiratory signs, with higher MS prevalence than MG. The positivities forMycoplasmaspp., MS, and MG were different among the orders studied, being the highest occurrence in birds of prey, followed by Galliformes and Piciformes. The presence of MG and MS in birds of Rio de Janeiro Zoo confirms the circulation of these agents and the need for further studies on the dissemination of mycoplasmas in zoos for the epidemiological analysis of these bacteria in these places.

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Section: Resultssupporting

confidence: 89%

Investigation of Mycoplasma spp. in birds of the Rio de Janeiro Zoo by isolation and PCR

et al. 2020

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“…The characterization of a few strains per each novel species is highly desirable, but not mandatory, by the current standards [22]. For example, a few recently described novel Mycoplasma species have been published based on the characterization of a single strain per each species [32,[53][54][55]. We believe that the description of novel species in the class Mollicutes based on only a single strain per each species could be sufficient in a case when the novel species found to be genetically distinct in comparison to other well-known species.…”

Section: Genome and Multilocus Sequence Analyses Phylogeny And Other ...mentioning

confidence: 99%

Mycoplasma miroungirhinis sp. nov. and Mycoplasma miroungigenitalium sp. nov., isolated from northern elephant seals (Mirounga angustirostris), Mycoplasma phocoenae sp. nov., isolated from harbour porpoise (Phocoena phocoena), and Mycoplasma phocoeninasale sp. nov., isolated from harbour porpoise and California sea lions (Zalophus californianus)

Volokhov

Furtak

Blom

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Seven novel independent strains of Mycoplasma species were isolated from northern elephant seals (ES2806-NAST, ES2806-GENT, ES3157-GEN-MYC and ES3225-GEN-MYC), a harbour porpoise (C264-GENT and C264-NAST), and a California sea lion (CSL7498). These strains were phenotypically and genetically characterized and compared to the known Mycoplasma species. Four strains (C264-GENT, C264-NAST, CSL7498 and ES2806-NAST) hydrolysed arginine but not urea and did not produce acid from carbohydrates. Strains ES2806-GENT, ES3157-GEN-MYC and ES3225-GEN-MYC did not produced acid from carbohydrates and did not hydrolyse arginine or urea; hence, it is assumed that organic acids are used as the energy source for them. All were isolated and propagated in ambient air supplemented with 5±1 % CO2 at +35–37 °C using either SP4 or PPLO medium. Colonies on solid medium showed a typical fried-egg appearance and transmission electron microscopy revealed a typical mycoplasma cellular morphology. The complete genomes were sequenced for all type strains. Average nucleotide and amino acid identity analyses showed that these novel strains were distant from the phylogenetically closely related Mycoplasma species. Based on these data, we propose four novel species of the genus Mycoplasma , for which the name Mycoplasma miroungirhinis sp. nov. is proposed with the type strain ES2806-NAST (=NCTC 14430T=DSM 110945T), Mycoplasma miroungigenitalium sp. nov. is proposed with the type strain ES2806-GENT (=NCTC 14429T=DSM 110944T) and representative strains ES3157-GEN-MYC and ES3225-GEN-MYC, Mycoplasma phocoenae sp. nov. is proposed with the type strain C264-GENT (=NCTC 14344T=DSM 110687T) and Mycoplasma phocoeninasale sp. nov. is proposed with the type strain C264-NAST (=NCTC 14343T=DSM 110688T) and representative strain CSL7498. The genome G+C contents are 24.06, 30.09, 28.49 and 29.05% and the complete genome sizes are 779 550, 815 486, 693 115, and 776 009 bp for strains ES2806-NAST, ES2806-GENT, C264-GENT and C264-NAST, respectively.

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“… 28 described a new species of mycoplasma, Mycoplasma seminis , that was isolated from semen of a gyrfalcon ( Falco rusticolus ) without any clinical signs of disease. Although mycoplasmas were isolated from raptors with clinical signs, many authors describe them as opportunistic pathogens or as a part of the physiological microbiota of the upper respiratory tract 29 – 31 .…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Mycoplasma spp. in wild birds: phylogenetic analysis and potential factors affecting distribution

Sawicka

Kursa

Bednarz³

et al. 2021

Sci Rep

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Different Mycoplasma species have been reported in avian hosts. However, the majority of studies focus on one particular species of Mycoplasma or one host. In our research, we screened a total of 1141 wild birds representing 55 species, 26 families, and 15 orders for the presence of mycoplasmas by conventional PCR based on the 16S rRNA gene. Selected PCR products were sequenced to perform the phylogenetic analysis. All mycoplasma-positive samples were tested for M. gallisepticum and M. synoviae, which are considered the major pathogens of commercial poultry. We also verified the influence of ecological characteristics of the tested bird species including feeding habits, habitat types, and movement patterns. The presence of Mycoplasma spp. was confirmed in 498 birds of 29 species, but none of the tested birds were positive for M. gallisepticum or M. synoviae. We found possible associations between the presence of Mycoplasma spp. and all investigated ecological factors. The phylogenetic analysis showed a high variability of Mycoplasma spp.; however, some clustering of sequences was observed regarding particular bird species. We found that wild migratory waterfowl, particularly the white-fronted goose (Anser albifrons) and mallard (Anas platyrhynchos) could be reservoirs and vectors of mycoplasmas pathogenic to commercial waterfowl.

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Mycoplasma hafezii sp. nov., isolated from the trachea of a peregrine falcon (Falco peregrinus)

Cited by 12 publications

References 18 publications

Investigation of Mycoplasma spp. in birds of the Rio de Janeiro Zoo by isolation and PCR

Investigation of Mycoplasma spp. in birds of the Rio de Janeiro Zoo by isolation and PCR

Occurrence of Mycoplasma spp. in wild birds: phylogenetic analysis and potential factors affecting distribution

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