Mycorrhizal frequency, physiological parameters, and yield of strawberry plants inoculated with endomycorrhizal fungi and rhizosphere bacteria

Abstract

Due to the impoverishment of agricultural and horticultural soils and replant diseases, there is a need to use bioproducts and beneficial microorganisms in order to improve the quality of soils and growth substrates. For this reason, research was undertaken to assess the impact of arbuscular mycorrhizal fungi and rhizosphere bacteria on changes in soil microbiology, the degree of colonization of plant roots by mycorrhizal fungi, selected physiological parameters, and fruit quality and yield of the strawberry cultivar "Rumba." The plants were inoculated with the mycorrhizal preparation Mykoflor (Rhizophagus irregularis, Funneliformis mosseae, Claroideoglomus etunicatum), MYC 800 (Rhizophagus intraradices), and the bacterial preparation Rhizocell C (Bacillus amyloliquefaciens IT45). The applied preparations increased the total number of bacteria and fungi in the soil and mycorrhizal frequency in the roots of the strawberry plants. They increased the chlorophyll "a" and total chlorophyll concentrations in the leaves as well as the rate of transpiration and CO 2 concentration in the intercellular spaces in the leaves. The plants treated with Rhizocell C and MYC 800 exhibited a higher CO 2 assimilation rate than control plants. The biopreparations increased chlorophyll fluorescence parameters such as maximum fluorescence (F M) and the maximum potential photochemical reaction efficiency in PS II (F V /F M). The influence of the species of rhizosphere bacteria and mycorrhizal fungi used in the experiment on the physiological traits of strawberry plants contributed, especially in the second year of the study, to increase the yield and mean weight of strawberry fruit.

The Effects of Bioinoculants Based on Mycorrhizal and Trichoderma spp. Fungi in an Apple Tree Nursery under Replantation Conditions

“…fungi. Our study did not confirm the results of the experiments conducted by other researchers, who observed that mycorrhization treatment increased the content of chlorophyll and carotenoids in the leaves of strawberries [40,60], cucumbers [56], and courgettes [61].…”

“…The authors found that the fungal population on the roots of the apple trees treated with the inoculant was over 50% greater than on plants which had not received the treatment. In the experiment conducted by Mikiciuk et al [40], the population was more than two times larger.…”

Effect of Arbuscular Mycorrhizal Fungi Isolated From Rock Phosphate Mine and Agricultural Soil on the Improvement of Wheat Plant Growth

“…The assessment of the colonization degree of the roots by arbuscular mycorrhizal fungi was performed with the Trouvelot method ( Trouvelot et al, 1986 ). This method is based on the assessment of mycorrhizal frequency (F%) and relative mycorrhizal intensity (M%) that are calculated according to the formula described by Mikiciuk et al (2019) .…”

A Preliminary Study on the Response of Confectionery Sunflower (Helianthus annuus L.) to Arbuscular Mycorrhizal Fungi (AMF)

“…As a second application method, mycorrhiza was applied by soil spraying before first irrigation at the 6-8 leaf stage. To water one plant, 260 cm 3 of the suspension was used, which corresponds to a dose of 8.75 mg of the preparation per plant (Mikiciuk et al, 2019).…”