Mycosynthesis of silver nanoparticles using <i>Aspergillus caespitosus</i>: Characterization, antimicrobial activities, cytotoxicity, and their performance as an antimicrobial agent for textile materials

El-Bendary, Magda A.; Moharam, Maysa E.; Hamed, Shimaa R.; El-Ola, S. M. Abo; Khalil, Samar; Mounier, Marwa M.; Roshdy, Amira M.; Allam, Mousa A.

doi:10.1002/aoc.6338

Cited by 10 publications

(4 citation statements)

References 45 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies reported the biosynthesis of AgNPs with the Aspergillus family, including A. terreus, A. fumigatus, A.oryzae, A.niger, A.flavus, A. clavatus, and A. conicus [29][30][31]. These fungal strains possess efficient mechanisms for secreting extracellular enzymes, which help in the synthesis process by reducing the enzymes extracellularly or intracellularly [32,33].…”

Section: Introductionmentioning

confidence: 99%

Bimetallic Assembled Silver Nanoparticles Impregnated in Aspergillus fumigatus Extract Damage the Bacterial Membrane Surface and Release Cellular Contents

et al. 2022

View full text Add to dashboard Cite

The bactericidal effects of nanomaterials play an essential role in cytoplasmic leakage, leading to bacterial cell death. In this study, silver nanoparticles (AgNPs) were synthesized using a fungal extract of Aspergillus fumigatus (A. fumigatus). The physicochemical properties of the bare and myco-synthesized AgNPs (MS-AgNPs) were examined by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, ultraviolet–visible (UV–vis) spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). XRD revealed the crystalline structure of the prepared NPs. The FTIR spectrum of the MS-AgNPs revealed the presence of the stretching vibrations of hydroxyl (−OH) and carbonyl groups (C=O). The UV results showed absorption from 450 nm to 590 nm, confirming the synthesis of the AgNPs. SEM and TEM showed rough cubic shapes (spheres), 20–60 nm in size, while EDX confirmed the presence of 60% Ag in the sample. The MS-AgNPs revealed the highest antibacterial activity against Staphylococcus aureus, with a zone of inhibition of 18.21 ± 2.1 mm, followed by Shigella dysenteriae and Salmonella typhi. The bimetallic-AgNPs played a vital role in cell membrane damage and the release of cellular contents, specifically nucleic acids and proteins. These results suggest that MS-AgNPs have promising antimicrobial capabilities and might be beneficial for an extensive array of biological applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Bimetallic Assembled Silver Nanoparticles Impregnated in Aspergillus fumigatus Extract Damage the Bacterial Membrane Surface and Release Cellular Contents

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Conversely, higher concentrations of AgNPs and lower average AgNP sizes are linked to higher and lower maximum wavelength values, respectively [ 87 ]. El-Bendary et al [ 88 ] detected a peak in the AgNPs spectra at 410 nm, providing evidence that AgNPs were produced by Aspergillus caespitosus. Here, Hashem et al [ 42 ] produced AgNPs by B. thuringiensis MAE 6 and had an SPR absorption band at 420 nm.…”

Section: Discussionmentioning

confidence: 99%

Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

Mekky,

Abdelaziz,

Youssef

et al. 2024

Medicina

View full text Add to dashboard Cite

Background and Objectives: Urinary tract infections [UTIs] are considered the third most known risk of infection in human health around the world. There is increasing appreciation for the pathogenicity of Gram-positive and Gram-negative strains in UTIs, aside from fungal infection, as they have numerous virulence factors. Materials and Methods: In this study, fifty urine samples were collected from patients suffering from UTI. Among the isolates of UTI microbes, six isolates were described as MDR isolates after an antibiotic susceptibility test carried out using ten different antibiotics. An alternative treatment for microbial elimination involved the use of biosynthesized silver nanoparticles (AgNPs) derived from Solanum lycopersicum [S. cumin]. Results: The sizes and shapes of AgNPs were characterized through TEM imaging, which showed spherical particles in a size range of 35–80 nm, of which the average size was 53 nm. Additionally, the silver nanoparticles (AgNPs) demonstrated inhibitory activity against Staphylococcus aureus (OR648079), exhibiting a 31 mm zone of inhibition at a minimum inhibitory concentration (MIC) of 4 mg/mL and a minimum bactericidal concentration (MBC) of 8 mg/mL. This was followed by Aspergillus niger (OR648075), which showed a 30 mm inhibition zone at an MIC of 16 mg/mL and a minimum fungicidal concentration (MFC) of 32 mg/mL. Then, Enterococcus faecalis (OR648078), Klebsiella pneumoniae (OR648081), and Acinetobacter baumannii (OR648080) each displayed a 29 mm zone of inhibition at an MIC of 8 mg/mL and an MBC of 16 mg/mL. The least inhibition was observed against Candida auris (OR648076), with a 25 mm inhibition zone at an MIC of 16 mg/mL and an MFC of 32 mg/mL. Furthermore, AgNPs at different concentrations removed DPPH and H2O2 at an IC50 value of 13.54 μg/mL. Also, AgNPs at 3 mg/mL showed remarkable DNA fragmentation in all bacterial strains except Enterococcus faecalis. The phytochemical analysis showed the presence of different active organic components in the plant extract, which concluded that rutin was 88.3 mg/g, garlic acid was 70.4 mg/g, and tannic acid was 23.7 mg/g. Finally, AgNPs concentrations in the range of 3–6 mg/mL showed decreased expression of two of the fundamental genes necessary for biofilm formation within Staphylococcus aureus, fnbA (6 folds), and Cna (12.5 folds) when compared with the RecA gene, which decreased by one-fold when compared with the control sample. These two genes were submitted with NCBI accession numbers [OR682119] and [OR682118], respectively. Conclusions: The findings from this study indicate that biosynthesized AgNPs from Solanum lycopersicum exhibit promising antimicrobial and antioxidant properties against UTI pathogens, including strains resistant to multiple antibiotics. This suggests their potential as an effective alternative treatment for UTIs. Further research is warranted to fully understand the mechanisms of action and to explore the therapeutic applications of these nanoparticles in combating UTIs.

show abstract

“…New drugs to combat this problem are therefore in great demand, but as past experience indicates, the time for resistance to new drugs to develop is often short. Conventionally, antibacterial drugs have been developed on the basis of their ability to inhibit bacterial multiplication, and this remains at the core of most approaches to discover new antibacterial drugs 1–5 . Nanotechnology and antibacterial materials have promise for the development of novel therapeutic interventions and effective preventive strategies against infections 6,7 .…”

Section: Introductionmentioning

confidence: 99%

Exploring the biomedical potential of chitosan‐ceria nanocomposites: synthesis and characterizations

Bashal,

Khalil,

Habeeb

et al. 2024

Applied Organom Chemis

View full text Add to dashboard Cite

The objective of this study was to synthesize and evaluate the biological effectiveness of cerium oxide (CeO2) against various bacterial and fungal strains. The chitosan‐CeO2 nanocomposite was comprehensively characterized. The Fourier transform infrared (FTIR) spectra displayed a combination of bands that provided evidence for the existence of a composite complex between the chitosan (CS) structure and the Ce–O bands originating from CeO2 nanoparticles. SEM measurements showed uniform dispersion across the whole CS surface, and the morphological changes were clearly observed as the CeO2 nanoparticle content was increased. In contrast, CS‐CeO2 demonstrates enhanced antibacterial efficacy against both Gram‐positive and Gram‐negative pathogens. The MTT method was utilized to assess the cytotoxicity of the examined CS‐CeO2, with the aim of evaluating the cell viability of various cancer cell lines under varying ultraviolet (UV) exposures. The enhanced penetration of CS and CeO2 nanocomposites into cancer cells during their anticancer activity, even in the absence of UV light, led to an increased rate of cell death. The administration of greater dosages of CS and its CeO2 nanocomposites led to an increased generation of reactive oxygen species (ROS), which was observed to be associated with an enhancement in the efficacy of anticancer activity. The impact was additionally amplified through exposure to UV radiation. The nanocomposite thin films demonstrated much higher antioxidant activity against the DPPH radical as compared to ordinary vitamin C. The aforementioned observation was derived from an evaluation of the antioxidant properties of the subject utilizing the DPPH free radical scavenging technique.

show abstract

Mycosynthesis of silver nanoparticles using Aspergillus caespitosus: Characterization, antimicrobial activities, cytotoxicity, and their performance as an antimicrobial agent for textile materials

Cited by 10 publications

References 45 publications

Bimetallic Assembled Silver Nanoparticles Impregnated in Aspergillus fumigatus Extract Damage the Bacterial Membrane Surface and Release Cellular Contents

Bimetallic Assembled Silver Nanoparticles Impregnated in Aspergillus fumigatus Extract Damage the Bacterial Membrane Surface and Release Cellular Contents

Unravelling the Antimicrobial, Antibiofilm, Suppressing Fibronectin Binding Protein A (fnba) and cna Virulence Genes, Anti-Inflammatory and Antioxidant Potential of Biosynthesized Solanum lycopersicum Silver Nanoparticles

Exploring the biomedical potential of chitosan‐ceria nanocomposites: synthesis and characterizations

Contact Info

Product

Resources

About