Myeloid cells regulate plasma LDL-cholesterol levels

Bazioti, Venetia; Rose, Anouk M. La; Westerterp, Marit

doi:10.1097/mol.0000000000000511

Cited by 4 publications

(4 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The latter would be similar to mouse models with hematopoietic or myeloid Abca1/Abcg1 deficiency or patients with myeloproliferative diseases that are also characterized by a decrease in VLDL/LDL-cholesterol, accompanied by HSPC mobilization and splenomegaly. It has been proposed that the VLDL/LDLcholesterol uptake by hematopoietic cells drives HSPC proliferation in the spleen, as we recently reviewed (55).…”

Section: Discussionmentioning

confidence: 99%

Elevated granulocyte-colony stimulating factor and hematopoietic stem cell mobilization in Niemann-Pick type C1 disease

Groenen

Rose

et al. 2022

Journal of Lipid Research

Self Cite

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: Discussionmentioning

confidence: 99%

Elevated granulocyte-colony stimulating factor and hematopoietic stem cell mobilization in Niemann-Pick type C1 disease

Groenen

Rose

et al. 2022

Journal of Lipid Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the spleen participates in the modulation of lipid metabolism and plasma lipids content, mechanisms relevant to the onset of atherosclerotic complications and cardiovascular diseases [ 10 , 17 – 20 ]. Elevated plasma LDL-cholesterol levels have been observed both in humans and animal models after splenectomy, suggesting a preeminent role for the spleen in LDL catabolism [ 21 – 23 ].…”

Section: Introductionmentioning

confidence: 99%

High-fat diet activates splenic NOD1 and enhances neutrophil recruitment and neutrophil extracellular traps release in the spleen of ApoE-deficient mice

Fernández-García

González-Ramos

Avendaño-Ortíz

et al. 2022

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

In the course of atherogenesis, the spleen plays an important role in the regulation of extramedullary hematopoiesis, and in the control of circulating immune cells, which contributes to plaque progression. Here, we have investigated the role of splenic nucleotide-binding oligomerization domain 1 (NOD1) in the recruitment of circulating immune cells, as well as the involvement of this immune organ in extramedullary hematopoiesis in mice fed on a high-fat high-cholesterol diet (HFD). Under HFD conditions, the absence of NOD1 enhances the mobilization of immune cells, mainly neutrophils, from the bone marrow to the blood. To determine the effect of NOD1-dependent mobilization of immune cells under pro-atherogenic conditions, Apoe−/− and Apoe−/−Nod1−/− mice fed on HFD for 4 weeks were used. Splenic NOD1 from Apoe−/− mice was activated after feeding HFD as inferred by the phosphorylation of the NOD1 downstream targets RIPK2 and TAK1. Moreover, this activation was accompanied by the release of neutrophil extracellular traps (NETs), as determined by the increase in the expression of peptidyl arginine deiminase 4, and the identification of citrullinated histone H3 in this organ. This formation of NETs was significantly reduced in Apoe−/−Nod1−/− mice. Indeed, the presence of Ly6G+ cells and the lipidic content in the spleen of mice deficient in Apoe and Nod1 was reduced when compared to the Apoe−/− counterparts, which suggests that the mobilization and activation of circulating immune cells are altered in the absence of NOD1. Furthermore, confirming previous studies, Apoe−/−Nod1−/− mice showed a reduced atherogenic disease, and diminished recruitment of neutrophils in the spleen, compared to Apoe−/− mice. However, splenic artery ligation reduced the atherogenic burden in Apoe−/− mice an effect that, unexpectedly was lost in Apoe−/−Nod1−/− mice. Together, these results suggest that neutrophil accumulation and activity in the spleen are driven in part by NOD1 activation in mice fed on HFD, contributing in this way to regulating atherogenic progression.

show abstract

“…This is because the spleen harbors numerous highly differentiated anatomical structures and cells that allow specific functions due to its direct connection to systemic circulation and further links with the nervous and immune systems (6). Anatomically, the spleen architecture includes heterogeneous populations of stromal, immune and endothelial cells, organized in domains with specific microcirculation (7)(8)(9)(10)(11). In addition, some particular immune functions have been ascribed to this versatile organ, such as removal of non-opsonized bacteria, blood cells clearance and sensing the presence of circulating pathogen-associated molecular patterns (PAMPS).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, leukocyte lineages derived from hematopoietic cells, mainly neutrophils releasing neutrophil extracellular traps (NETs) and macrophages, cells that in turn express NOD molecules, exert key roles in the mobilization of hematopoietic cells under pro-inflammatory conditions. In addition to these immune functions, the spleen participates in the modulation of lipid metabolism and plasma lipids content, mechanisms relevant in the onset of atherosclerotic complications and cardiovascular diseases (10,(15)(16)(17)(18). In fact, elevated plasma LDL-cholesterol levels have been observed both in humans and animal models after splenectomy, suggesting a preeminent role for the spleen in LDL catabolism (19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

High Fat Diet Activates Splenic NOD1 and Enhances Neutrophil Recruitment and Neutrophil Extracellular Traps Release in the Spleen of ApoE-Deficient Mice

Fernández-García

González-Ramos

Avendaño-Ortíz

et al. 2022

Preprint

View full text Add to dashboard Cite

In the course of atherogenesis, the spleen plays an important role in the regulation of extramedullary hematopoiesis and in the control of circulating immune cells, which contributes to plaque progression. Here, we have investigated the role of splenic nucleotide-binding oligomerization domain 1 (NOD1) in the recruitment of circulating immune cells as well as the involvement of this immune organ in extramedullary hematopoiesis in mice fed a high-fat high-cholesterol diet (HFD). Under HFD conditions, the absence of NOD1 enhances the mobilization of immune cells, mainly neutrophils, from the bone marrow to the blood. To determine the effect of NOD1-dependent mobilization of immune cells under pro-atherogenic conditions, Apoe -/- and Apoe -/- Nod1 -/- mice fed HFD for 4 weeks were used. Splenic NOD1 from Apoe -/- mice was activated after feeding HFD as inferred by the phosphorylation of the NOD1 downstream targets RIPK2 and TAK1. Moreover, this activation was accompanied by the release of neutrophil extracellular traps (NETs), as determined by the increase in the expression of peptidyl arginine deiminase 4, and the identification of citrullinated histone H3 in this organ. This formation of NETs was significantly reduced in Apoe -/- Nod1 -/- mice. Indeed, the presence of Ly6G + cells and the lipidic content in the spleen of mice deficient in Apoe and Nod1 was reduced when compared to the Apoe -/- counterparts, which suggests that the mobilization and activation of circulating immune cells is altered in the absence of NOD1. Furthermore, confirming previous studies, Apoe -/- Nod1 -/- mice showed a reduced atherogenic disease and a diminished recruitment of neutrophils in the spleen, compared to Apoe -/- mice. However, splenic artery-ligation reduced the atherogenic burden in Apoe -/- mice an effect that, unexpectedly was lost in Apoe -/- Nod1 -/- mice. Together, these results suggest that neutrophil accumulation and activity in the spleen is driven in part by NOD1 activation in mice fed HFD, contributing in this way to regulate atherogenic progression.

show abstract

Myeloid cells regulate plasma LDL-cholesterol levels

Abstract: Enhanced hematopoiesis and monocytosis may accelerate atherogenesis. Studies on these processes may lead to the identification of new therapeutic targets for cardiovascular diseases.

Cited by 4 publications

References 62 publications

Elevated granulocyte-colony stimulating factor and hematopoietic stem cell mobilization in Niemann-Pick type C1 disease

Elevated granulocyte-colony stimulating factor and hematopoietic stem cell mobilization in Niemann-Pick type C1 disease

High-fat diet activates splenic NOD1 and enhances neutrophil recruitment and neutrophil extracellular traps release in the spleen of ApoE-deficient mice

High Fat Diet Activates Splenic NOD1 and Enhances Neutrophil Recruitment and Neutrophil Extracellular Traps Release in the Spleen of ApoE-Deficient Mice

Contact Info

Product

Resources

About