Myocardial fibrosis assessment by semiquantitative, point‐counting and computer‐based methods in patients with heart muscle disease: a comparative study

Vasiljević, Jovan; Popović, Zoran B.; Otašević, Petar; Popović, Zoran V.; Vidakovic, Radislav; Mirić, Milutin; Nešković, Aleksandar N.

doi:10.1046/j.1365-2559.2001.01101.x

Cited by 37 publications

(34 citation statements)

References 29 publications

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“…Two independent observers graded the amount of fibrosis in percentage, and their values were then averaged. It has been previously shown that this type of fibrosis estimation corresponds well with more quantitative ways of its measurement (27). Finally, to obtain histological wall thickness, we used ImagePro Plus (version 5.1) software to first segment the LV wall as described and then to calculate average segmental wall thickness.…”

Section: Methodsmentioning

confidence: 98%

Speckle-tracking echocardiography correctly identifies segmental left ventricular dysfunction induced by scarring in a rat model of myocardial infarction

Popović

Benejam

Bian

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

110

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MS. Speckletracking echocardiography correctly identifies segmental left ventricular dysfunction induced by scarring in a rat model of myocardial infarction. Am J Physiol Heart Circ Physiol 292: H2809 -H2816, 2007. First published February 2, 2007; doi:10.1152/ajpheart.01176.2006.-Speckle-tracking echocardiography (STE) uses a two-dimensional echocardiographic image to estimate two orthogonal strain components. The aim of this study was to assess sensitivity of circumferential (Scirc) and radial (Srad) strains to infarct-induced left ventricular (LV) remodeling and scarring of the LV in a rat. To assess the relationship among Scirc, Srad, and scar size, two-dimensional echocardiographic LV short-axis images (12 MHz transducer, Vivid 7 echo machine) were collected in 34 Lewis rats 4 to 10 wk after ligation of the left anterior descending artery. Percent segmental fibrosis was assessed from histological LV cross sections stained by Masson trichrome. Ten normal rats served as echocardiographic controls. Scirc and Srad were assessed by STE. Histological data showed consistent scarring of anterior and lateral segments with variable extension to posterior and inferior segments. Both Scirc and Srad significantly decreased after myocardial infarction (P Ͻ 0.0001 for both). As anticipated, Scirc and Srad were lowest in the infarcted segments. Multiple linear regression showed that segmental S circ were similarly dependent on segmental fibrosis and end-systolic diameter (P Ͻ 0.0001 for both), whereas segmental S rad measurements were more dependent on end-systolic diameter (P Ͻ 0.0001) than on percent fibrosis (P Ͻ 0.002). STE correctly identifies segmental LV dysfunction induced by scarring that follows myocardial infarction in rats.infarction; collagen; contractility RECENT ADVANCES in noninvasive imaging of the left ventricle (LV) have enabled the quantification of regional function with an accuracy and simplicity that were not available previously. One of these newer methods is speckle-tracking echocardiography (STE). STE uses a standard two-dimensional echocardiographic image to estimate two orthogonal strain components (25). This method has a potential to revolutionize noninvasive assessment of regional ventricular (and possibly atrial) function (25). Although similar data can be extracted by Doppler tissue echocardiography (2), the value of this method is the freedom of the insonation angle and an ability to adopt a local coordinate system for strain measurement.Although STE was recently validated in a variety of settings (1,5,10,17, 22), the relationship of strain to underlying muscle histology is uncertain. It is unknown as to what measure a decreased strain represents muscle loss, presence of ischemia, or a simple contractility decrease. Finally, the applicability of this method to small-animal echocardiography is unknown.The aim of this study was to assess sensitivity of circumferential (S circ ) and radial (S rad ) midwall strain components to LV remodeling and scarring induced by the myocardial infarction (MI...

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Section: Methodsmentioning

confidence: 98%

Speckle-tracking echocardiography correctly identifies segmental left ventricular dysfunction induced by scarring in a rat model of myocardial infarction

Popović

Benejam

Bian

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

110

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“…13 Both semiquantitative and quantitative methods were used as described 13,14 to report the condition of the myocardium.…”

Section: Morphometric Assessment Of Myocardial Conditionmentioning

confidence: 99%

“…A semiquantitative method was used as described 13 in the assessment of the condition of the myocardium and scored as follows: 0 ϭ normal; 1 ϭ mild degenerative changes, including vacuolation; 2 ϭ moderate degenerative changes, including widespread vacuolation with increased fibrosis; and 3 ϭ severe changes including necrosis, large areas of interstitial fibrosis, and significant loss of cardiomyocytes. The incidental occurrence of the epicardial fibrosis/calcification lesion that is common in A/J and other strains of mice was excluded from consideration in this study.…”

Section: Morphometric Assessment Of Myocardial Conditionmentioning

confidence: 99%

Dysferlin Deficiency and the Development of Cardiomyopathy in a Mouse Model of Limb-Girdle Muscular Dystrophy 2B

Chase

Cox

Burzenski

et al. 2009

The American Journal of Pathology

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Limb-girdle muscular dystrophy 2B, Miyoshi myopathy, and distal myopathy of anterior tibialis are severely debilitating muscular dystrophies caused by genetically determined dysferlin deficiency. In these muscular dystrophies, it is the repair, not the structure, of the plasma membrane that is impaired. Though much is known about the effects of dysferlin deficiency in skeletal muscle, little is known about the role of dysferlin in maintenance of cardiomyocytes. Recent evidence suggests that dysferlin deficiency affects cardiac muscle , leading to cardiomyopathy when stressed. However, neither the morphological location of dysferlin in the cardiomyocyte nor the progression of the disease with age are known. In this study, we examined a mouse model of dysferlinopathy using light and electron microscopy as well as echocardiography and conscious electrocardiography. We determined that dysferlin is normally localized to the intercalated disk and sarcoplasm of the cardiomyocytes. In the absence of dysferlin, cardiomyocyte membrane damage occurs and is localized to the intercalated disk and sarcoplasm. This damage results in transient functional deficits at 10 months of age, but, unlike in skeletal muscle, the cell injury is sublethal and causes only mild cardiomyopathy even at advanced

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“…In the myocardium, amyloid may be found surrounding individual myocytes, or in the form of focal nodular interstitial deposits that push aside and replace fibres, or both. Myocardial fibrosis may be assesed by different methods 46 In coronary vessels, it may involve all vascular layers and even cause luminal occlusion. The presence of amyloid can be confirmed by the apple green birefringence it gives under polarised light after Congo red staining, or by metachromasis with methyl violet, or ultraviolet fluorescence with thioflavin T (the latter may yield false positives).…”

Section: Cardiac Amyloidosismentioning

confidence: 99%

Endomyocardial biopsy: Yesterday, today and tomorrow

Vasiljević¹

2012

Mat Med

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The major goals of this presentation are to give the updated knowledge of primary heart muscle disease, cardiomyopathies (CMP), their classification, the diagnostic possibilities using endomyocardial biopsy (EMB) and to estimate the value of EMB in the choice of the right therapeutic approach. despite many controversies, EMB is today a widely accepted method, with low percentage of complications for analysing CMPs, and is considered to be a routine procedure in many cardiological centers. Inspite the fact that we started with the use of EMB in 80's, it is not performed often today in Belgrade, like many yeras ago (2) . It's difficult to say why ?The CMPs may be subdivided, according to the functional and structural features, into two groups: idiopathic and specific, or primary and secundary. Idipathic group is consisted of 5 subgroups: hypertrophic, dilated, restrictive, arrithmogenic right ventricular CMP and unclassified.specific CMPs may be subdivided into 8 groups mainly based on etiopathogenetic characteristics: infective, with viral myocarditis as the most common entity, metabolic, including endocrine disorders and infiltration and storage diseases. deficiency disorders and heart involvement in connective tissue disorders are also included. Granulomas, neoplasms and neuromuscular disorders are also wery often presented with cardiac disfunction and structural abnormalities. sensitivity and toxic reactions with long list of substances wich may affect the heart are probably the most present today. Finally, the last group represent miscellaneous systemic syndromes with heart affection.EMB has been increasingly used in the diagnosis of CMPs, with special influnce on diagnosis of heart transplant rejection, myocarditis, treatment modalities of different types of myocarditis, with low complication rate, considered effective, usefull and safe procedure.

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Myocardial fibrosis assessment by semiquantitative, point‐counting and computer‐based methods in patients with heart muscle disease: a comparative study

Abstract: Our data show good correlations between the three methods of myocardial fibrosis assessment. However, systematic differences between them emphasize that this should be taken into consideration when comparing results of the studies using different methods of fibrosis assessment.

Cited by 37 publications

References 29 publications

Speckle-tracking echocardiography correctly identifies segmental left ventricular dysfunction induced by scarring in a rat model of myocardial infarction

Speckle-tracking echocardiography correctly identifies segmental left ventricular dysfunction induced by scarring in a rat model of myocardial infarction

Dysferlin Deficiency and the Development of Cardiomyopathy in a Mouse Model of Limb-Girdle Muscular Dystrophy 2B

Endomyocardial biopsy: Yesterday, today and tomorrow

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