Myoepithelial cell staining by levanol fast cyanine 5RN applied to epoxy-embedded semithin sections.

El-Bardaie, Adel Mohamed; Nikai, Hiromasa

doi:10.1267/ahc.19.473

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Using electron microscopic observation and specific elastin staining, EL-Bardaie & Nikai (16) reported that immature elastic fibers or developing elastic tissue in salivary gland tumors primarily originated from neoplastic or modified myoepithelial cells. David and Buchner (28) stated that a higher incidence of elastic fibers occurred in pleomorphic adenomas and adenoid cystic carcinomas.…”

Section: Discussionmentioning

confidence: 99%

“…Modified myoepithelial cells in pleomorphie adenomas also display positive staining for S-100 protein and its subunits a and (3, as well as those in skin mixed tumors (9)(10)(11)(12)(13)(14). It has been pointed out that stromal tissue and modified myoepithelial cells contain elastins as one of their mesenchymal properties (15)(16)(17). No detailed description of the presence of vimentin in salivary pleomorphic adenomas has yet been reported, at least not with the use of monoclonal antibodies against vimentin.…”

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Monoclonal antibody‐detected vimentin distribution in pleomorphic adenomas of salivary glands

Yamada

Shinohara

Takai

et al. 1988

J Oral Pathology Medicine

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To evaluate the participation of myoepithelial components in pleomorphic adenomas, an immunohistochemical study was carried out using monoclonal antibodies to vimentin. Of a total of 80 cases, 50 tumors gave positive staining, 5 tumors very slight, and 25 tumors negative staining for vimentin. Localization patterns for vimentin were divided into 3 classes: 1) vimentin staining in fibrous stromal tissue; 2) variable intensities of vimentin staining were found in the outer layers of tumor cells in tubuloductal structures (some of which were spindle cells connected to modified myoepithelial cells which also gave variable vimentin staining); and 3) modified myoepithelial cells and chondroidlike cells displayed strongly positive staining for vimentin. Typical histologic features of pleomorphic ademomas, i.e., tubulo‐ductal or duct‐like structures were characterized by positive vimentin staining in outer tumor cells and by a positive keratin reaction in the luminal tumor cells. In tumors devoid of stromal connective tissues and the near absence of well‐developed, or modified myoepithelial cells, vimentin staining was absent.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Monoclonal antibody‐detected vimentin distribution in pleomorphic adenomas of salivary glands

Yamada

Shinohara

Takai

et al. 1988

J Oral Pathology Medicine

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Myoepithelium of salivary glands

Redman¹

1994

Microscopy Res & Technique

154

146

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In salivary glands and other exocrine organs, there are starfish-shaped cells that lie between the basal lamina and the acinar and ductal cells. These have structural features of both epithelium and smooth muscle cells, and so are called myoepithelial cells. Their functions include contraction when the gland is stimulated to secrete, compressing or reinforcing the underlying parenchymal cells, thus aiding in the expulsion of saliva and preventing damage to the other cells. They also may aid in the propagation of secretory and other stimuli. Their common developmental origin with the basal cells of the larger ducts is displayed in the mature glands by shared structural and immunohistochemical features, but most such basal cells do not have the distinguishing features of myoepithelial cells, such as myofibrils. Although myoepithelial cells can be identified by light microscopy through enzyme histochemistry and special stains and immunohistochemistry for their myofibrils, these techniques can be misleading in salivary gland neoplasms. Thus, the most reliable means of identifying neoplastic myoepithelial cells is with a combination of histochemistry and electron microscopy. The extent to which these cells are derived from undifferentiated stem cells in both normal and neoplastic growth is controversial. The presentation here of transmission electron microscopy (TEM) of well-differentiated myoepithelial cells in mitotic division indicates that stem cells are not necessarily the only source of myoepithelial cells in the later stages of salivary gland development or in neoplasia.

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Application of Levanol fast cyanine 5RN stain for demonstrating rhabdomyoblasts in epoxy‐embedded tumors

El-Bardaie

1993

J Oral Pathology Medicine

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To facilitate the survey of actomyosin-containing cells in soft tissue sarcomas with skeletal muscle differentiation, the tannic acid-phosphomolybdic acid-Levanol fast cyanine 5RN procedure was applied to plastic-free (deresined) thick sections (2-3 microns) obtained from epoxy blocks conventially prepared for transmission electron microscopy. By light microscopy, rhabdomyoblasts of varying configuration revealed intense dark blue staining reaction. The stained sections were used for orientation and direct interpretation of the ultrastructural material.

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Myoepithelial cell staining by levanol fast cyanine 5RN applied to epoxy-embedded semithin sections.

Cited by 3 publications

References 12 publications

Monoclonal antibody‐detected vimentin distribution in pleomorphic adenomas of salivary glands

Monoclonal antibody‐detected vimentin distribution in pleomorphic adenomas of salivary glands

Myoepithelium of salivary glands

Application of Levanol fast cyanine 5RN stain for demonstrating rhabdomyoblasts in epoxy‐embedded tumors

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