Myogenic cell lineages

Stockdale, Frank E.

doi:10.1016/0012-1606(92)90068-r

Cited by 341 publications

(240 citation statements)

References 108 publications

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“…The cells are thought to be derived from satellite cells, which are mononucleated cells lying along the muscle fibers in the normal tissue, and exist throughout the adult life as a potential source of new myoblasts. When muscular tissues receive injury or some dystrophic stimuli, the satellite cells divide and differentiate into myoblasts and fuse to form muscle fibers (for review see Stockdale, 1992). In our preliminary experiments using parental C2 cells, BMP-2 also inhibited myotube formation and induced ALP activity and osteocalcin production.…”

Section: Discussionmentioning

confidence: 90%

Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]

Katagiri

Yamaguchi

Komaki

et al. 1994

The Journal of Cell Biology

1,377

983

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Abstract. The implantation of bone morphogenetic protein (BMP) into muscular tissues induces ectopic bone formation at the site of implantation. To investigate the mechanism underlying this process, we examined whether recombinant bone morphogenetic protein-2 (BMP-2) converts the differentiation pathway of the clonal myoblastic cell line, C2C12, into that of osteoblast lineage. Incubating the cells with 300 ng/ml of BMP-2 for 6 d almost completely inhibited the formation of the multinucleated myotubes expressing troponin T and myosin heavy chain, and induced the appearance of numerous alkaline phosphatase (ALP)-positive cells. BMP-2 dose dependently induced ALP activity, parathyroid hormone (PTH)-dependent 3',5'-cAMP production, and osteocalcin production at concentrations above 100 ng/ml. The concentration of BMP-2 required to induce these osteoblastic phenotypes was the same as that required to almost completely inhibit myotube formation. Incubating primary muscle cells with 300 ng/ml of BMP-2 for 6 d also inhibited myotube formation, whereas induced ALP activity and osteocalcin production. Incubation with 300 ng/ml of BMP-2 suppressed the expression of mRNA for muscle creatine kinase within 6 h, whereas it induced mRNA expression for ALP, PTH/PTH-related protein (PTHrP) receptors, and osteocalcin within 24--48 h. BMP-2 completely inhibited the expression of myogenin mRNA by day 3. By day 3, BMP-2 also inhibited the expression of MyoD mRNA, but it was transiently stimulated 12 h after exposure to BMP-2. Expression of Id-1 mRNA was greatly stimulated by BMP-2. When C2C12 cells pretreated with BMP-2 for 6 d were transferred to a colony assay system in the absence of BMP-2, more than 84 % of the colonies generated became troponin T-positive and ALP activity disappeared. TGF-/31 also inhibited myotube formation in C2C12 cells, and suppressed the expression of myogenin and MyoD mRNAs without inducing that of Id-1 mRNA. However, no osteoblastic phenotype was induced by TGF-/31 in C2C12 cells. TGF-/31 potentiated the inhibitory effect of BMP-2 on myotube formation, whereas TGF-/31 reduced ALP activity and osteocalcin production induced by BMP-2 in C2C12 cells. These results indicate that BMP-2 specifically converts the differentiation pathway of C2C12 myoblasts into that of osteoblast lineage cells, but that the conversion is not heritable.S EVERAL lines of evidence indicate that osteoblasts, chondrocytes, myocytes, and adipocytes are all derived from a common progenitor cell called undifferentiated mesenchymal cells (Taylor and Jones, 1979; Grigoriadis et al., 1988Grigoriadis et al., , 1990 Yamaguchi and Kahn, 1991). During the process of their differentiation, progenitor cells acquire specific phenotypes depending upon the differentiated cell types under the control of respective regulatory factors (for reviews see Rodan and Rodan, 1984;Owen, 1988; Wlodar-Address all correspondence to Dr. Tatsuo Suda, Department of Biochemistry, School of Dentistry, Showa University, 1-5-8 Hatanodai, Tokyo 142, ski, 1990). The di...

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Section: Discussionmentioning

confidence: 90%

Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]

Katagiri

Yamaguchi

Komaki

et al. 1994

The Journal of Cell Biology

1,377

983

View full text Add to dashboard Cite

show abstract

“…Skeletal muscle development is a multistep process that involves commitment of multipotential, mesodermal precursors to a muscle cell fate, followed by myoblast proliferation, activation of muscle specific genes, and fusion to form multinucleated muscle fibers (Stockdale, 1992;Miller et al, 1999). C2C12 myoblasts provide a well-established in vitro model for muscle differentiation (Yamaguchi, 1995;Cossins et al, 2002;Liu et al, 2002).…”

Section: Model For Cell Cycle Withdrawal In C2c12 Myoblastsmentioning

confidence: 99%

“…Skeletal muscle formation during development is a multistep process that involves the determination of multipotential, mesodermal cells to give rise to myoblasts, withdrawal of the myoblasts from the cell cycle, and differentiation into muscle fibers (Stockdale, 1992;Miller et al, 1999). These processes are controlled by muscle-specific transcriptional regulators that determine cell fate and differentiation and by external signals that couple myogenesis to development and growth of the organism (Miller et al, 1999;Bailey et al, 2001;McKinsey et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide examination of myoblast cell cycle withdrawal during differentiation

Shen

Collier

Hlaing

et al. 2002

Developmental Dynamics

107

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Skeletal and cardiac myocytes cease division within weeks of birth. Although skeletal muscle retains limited capacity for regeneration through recruitment of satellite cells, resident populations of adult myocardial stem cells have not been identified. Because cell cycle withdrawal accompanies myocyte differentiation, we hypothesized that C2C12 cells, a mouse myoblast cell line previously used to characterize myocyte differentiation, also would provide a model for studying cell cycle withdrawal during differentiation. C2C12 cells were differentiated in culture medium containing horse serum and harvested at various time points to characterize the expression profiles of known cell cycle and myogenic regulatory factors by immunoblot analysis. BrdU incorporation decreased dramatically in confluent cultures 48 hr after addition of horse serum, as cells started to form myotubes. This finding was preceded by up-regulation of MyoD, followed by myogenin, and activation of Bcl-2. Cyclin D1 was expressed in proliferating cultures and became undetectable in cultures containing 40% fused myotubes, as levels of p21 WAF1/Cip1 increased and ␣-actin became detectable. Because C2C12 myoblasts withdraw from the cell cycle during myocyte differentiation following a course that recapitulates this process in vivo, we performed a genome-wide screen to identify other gene products involved in this process. Using microarrays containing ϳ10,000 minimally redundant mouse sequences that map to the UniGene database of the National Center for Biotechnology Information, we compared gene expression profiles between proliferating, differentiating, and differentiated C2C12 cells and verified candidate genes demonstrating differential expression by RT-PCR. Cluster analysis of differentially expressed genes revealed groups of gene products involved in cell cycle withdrawal, muscle differentiation, and apoptosis. In addition, we identified several genes, including DDAH2 and Ly-6A, whose expression specifically was up-regulated during cell cycle withdrawal coincident with early myoblast differentiation. Developmental Dynamics 226:128 -138, 2003.

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“…A materials constraint of most conventional manmade micromachined topographical substrates is that the features are not dynamic [4] as microenvironments in vivo often are [1,[5][6][7][8]. Reconfigurable surfaces have been developed to a much lesser degree and even then mostly for regulating surface chemistry not surface topography [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Reversible on-demand cell alignment using reconfigurable microtopography

2008

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Traditional cell culture substrates consist of static, flat surfaces although in vivo, cells exist on various dynamic topographies. We report development of a reconfigurable microtopographical system compatible with cell culture that is comprised of reversible wavy microfeatures on poly (dimethylsiloxane). Robust reversibility of the wavy micropattern is induced on the cell culture customized substrate by first plasma oxidizing the substrate to create a thin, brittle film on the surface and then applying and releasing compressive strain, to introduce and remove the microfeatures, respectively. The reversible topography was able to align, unalign, and realign C2C12 myogenic cell line cells repeatedly on the same substrate within 24 h intervals, and did not inhibit cell differentiation. The flexibility and simplicity of the materials and methods presented here provide a broadly applicable capability by which to investigate and compare dynamic cellular processes not yet easily studied using conventional in vitro culture substrates.

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Myogenic cell lineages

Cited by 341 publications

References 108 publications

Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]

Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]

Genome‐wide examination of myoblast cell cycle withdrawal during differentiation

Reversible on-demand cell alignment using reconfigurable microtopography

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