Myoglobin microplate assay to evaluate prevention of protein peroxidation

Marques, Sara S.; Magalhães, Luís M.; Mota, Ana I.P.; Soares, Tânia R.; Korsak, Barbara; Reis, Salette; Segundo, Marcela A.

doi:10.1016/j.jpba.2015.06.006

Cited by 6 publications

(2 citation statements)

References 38 publications

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“…As the loss of the red colour in fish meat is mainly linked to the oxidation of oxymyoglobin (OxyMb), an ORAC assay was performed to assess the ability of SOD Ap -containing extract to protect myoglobin from oxidation, using the compound 2,2′-Azobis(2-amidinopropane) dihydrochloride (AAPH) as oxidant [ 35 ]. Scalar dilutions of WT and transgenic SOD Ap tomato extracts were incubated with myoglobin in the presence of AAPH, and the absorbance decay, due to the oxidation of the heme group of the protein caused by the oxidant, was measured at 409 nm.…”

Section: Resultsmentioning

confidence: 99%

Recombinant Expression of Archaeal Superoxide Dismutases in Plant Cell Cultures: A Sustainable Solution with Potential Application in the Food Industry

Gogliettino

Arciello²,

Cillo

et al. 2022

Antioxidants

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Superoxide dismutase (SOD) is a fundamental antioxidant enzyme that neutralises superoxide ions, one of the main reactive oxygen species (ROS). Extremophile organisms possess enzymes that offer high stability and catalytic performances under a wide range of conditions, thus representing an exceptional source of biocatalysts useful for industrial processes. In this study, SODs from the thermo-halophilic Aeropyrum pernix (SODAp) and the thermo-acidophilic Saccharolobus solfataricus (SODSs) were heterologously expressed in transgenic tomato cell cultures. Cell extracts enriched with SODAp and SODSs showed a remarkable resistance to salt and low pHs, respectively, together with optimal activity at high temperatures. Moreover, the treatment of tuna fillets with SODAp-extracts induced an extension of the shelf-life of this product without resorting to the use of illicit substances. The results suggested that the recombinant plant extracts enriched with the extremozymes could find potential applications as dietary supplements in the nutrition sector or as additives in the food preservation area, representing a more natural and appealing alternative to chemical preservatives for the market.

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Section: Resultsmentioning

confidence: 99%

Recombinant Expression of Archaeal Superoxide Dismutases in Plant Cell Cultures: A Sustainable Solution with Potential Application in the Food Industry

Gogliettino

Arciello²,

Cillo

et al. 2022

Antioxidants

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show abstract

“…The two main methods most commonly applied to calculate ORAC values and evaluate the radical scavenging effects of antioxidants are namely lag time assessment [14][15][16][17] and net area under curve (net AUC) calculation [18][19][20][21][22]. Both methods for data analysis have been used in several studies, affording complementary information on the elucidation of antioxidant interaction with 2,2 -azobis(2-amidinopropane) dihydrochloride (AAPH) derived radicals using pyranine and FL as target molecules [13,[23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Exploiting Kinetic Features of ORAC Assay for Evaluation of Radical Scavenging Capacity

et al. 2023

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The analysis and interpretation of data retrieved from Oxygen Radical Absorbance Capacity (ORAC) assays represent a challenging task. ORAC indexes originate from different mathematical approaches often lacking correct elucidation of kinetic features concerning radical scavenging reactions by antioxidant compounds. In this work, the expression of ORAC values as area under fluorescein (FL) decay curves (AUC) and lag time are critically compared. This multi-parametric analysis showed the extension of radical scavenging reactions beyond the lag time period for caffeic acid, gallic acid, reduced glutathione and quercetin, extending their antioxidant protection of FL. Ethanol delayed the reaction of both FL and antioxidant compounds with free radical species generated from 2,2′-azobis(2-amidinopropane) dihydrochloride thermolysis. Trolox equivalent values, commonly used to express ORAC values, were more affected by the differences in radical scavenging kinetics between the reference and the tested antioxidant compounds when calculated from AUC than from lag time. These findings stressed the importance of choosing calibrator compounds presenting ORAC kinetics similar to samples to prevent biased estimation of the antioxidant capacity. Additionally, the framework proposed here provides a sustainable analytical method for the evaluation of antioxidant capacity, with an AGREE score of 0.73.

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The effects of rosmarinic acid on oxidative stress parameters and inflammatory cytokines in lipopolysaccharide-induced peripheral blood mononuclear cells

et al. 2020

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Myoglobin microplate assay to evaluate prevention of protein peroxidation

Cited by 6 publications

References 38 publications

Recombinant Expression of Archaeal Superoxide Dismutases in Plant Cell Cultures: A Sustainable Solution with Potential Application in the Food Industry

Recombinant Expression of Archaeal Superoxide Dismutases in Plant Cell Cultures: A Sustainable Solution with Potential Application in the Food Industry

Exploiting Kinetic Features of ORAC Assay for Evaluation of Radical Scavenging Capacity

The effects of rosmarinic acid on oxidative stress parameters and inflammatory cytokines in lipopolysaccharide-induced peripheral blood mononuclear cells

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