Myometrial activity around estrus in sows: spontaneous activity and effects of estrogens, cloprostenol, seminal plasma and clenbuterol

Langendijk, P.; Bouwman, E.G.; Soede, N.M.; Taverne, M.A.M.; Kemp, B.

doi:10.1016/s0093-691x(02)00657-x

Cited by 45 publications

(36 citation statements)

References 23 publications

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“…In the sow myometrium, uterine contractions have been shown to increase around oestrus (Scheerboom et al 1987, Langendijk et al 2002. In the myometrium at oestrus we found high levels of ERa in both cyclic and inseminated sows in our previous studies (Sukjumlong et al 2003(Sukjumlong et al , 2004b and high levels of PR in both experimental groups of sows in the present study.…”

Section: Discussionsupporting

confidence: 72%

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Sukjumlong¹,

Dalin²,

Sahlin³

et al. 2005

Reproduction

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Physiological changes in the sow uterus involve the regulation by progesterone and its receptor proteins (PR). Therefore, the aim of the present study was to investigate the localization of PR during different stages of the oestrous cycle and in inseminated sows during early pregnancy by use of immunohistochemistry. Uterine samples were collected from cyclic and inseminated sows at different stages of the oestrous cycle and early pregnancy. The samples were fixed in 10% formaldehyde and embedded in paraffin. Immunohistochemistry was done by use of a mouse monoclonal antibody to PR. The highest PR immunostaining in the surface epithelium was observed at oestrus/5 -6 h after artificial insemination (AI) and early dioestrus/70 h after AI. In the glandular epithelium, the highest level of PR was found at oestrus with the lowest at late dioestrus/d 19. Higher levels of PR were observed in inseminated groups compared with cyclic sows. In the myometrium, a high level of PR was found at oestrus, while stromal PR cells were constantly present throughout the oestrous cycle and at different stages of early pregnancy. In conclusion, this study shows that the immunopresence of PR in the sow uterus differed between uterine compartments at the same reproductive stage. Differences were also found for some uterine compartments between cyclic and inseminated/early pregnant sows. The relatively consistent immunostaining of PR in the stroma strengthens a stromal role in the regulation of physiological activities in the sow uterus during the oestrous cycle as well as early pregnancy.

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Section: Discussionsupporting

confidence: 72%

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Sukjumlong¹,

Dalin²,

Sahlin³

et al. 2005

Reproduction

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“…Spontaneous activity of the uterine myometrium, in terms of contraction tension, frequency and amplitude, is changing during the estrous cycle, mating or insemination, pregnancy and labour (Langendijk et al 2002, Kurowicka et al 2005. The contractile activity of the porcine myometrium is controlled by many factors including adrenergic, cholinergic and non-adrenergic non-cholinergic innervation (Łakomy 1987, Thilander et al 1989, Taneike et al 1991, Kaleczyc 1994, Majewski et al 1995, as well as endocrine, paracrine and autocrine factors (Kitazawa et al 1997, Cao et al 2002, Sukjumlong et al 2009, Franczak and Bogacki 2009.…”

Section: Introductionmentioning

confidence: 99%

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Markiewicz¹,

Kamińska²,

Bogacki³

et al. 2012

Polish Journal of Veterinary Sciences

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Recent studies show that a representative of phospholipids, namely lysophosphatidic acid (LPA) and its receptors (LPA 1-3 ) play a significant role in the reproductive processes, i. a, in the modulation of the uterine contractility. The participation of LPA 3 in the reproductive processes has been revealed in mice and has not been studied in gilts. Therefore, in the present study we investigated the role/action of LPA and its receptors LPA 1 , LPA 2 and LPA 3 on the contraction activity in the porcine uterus. The study was conducted on an experimental model in which the pig uterus consisted of the one whole uterine horn and a part of the second horn, both connected with the uterine corpus. Uterine strips consisting of the endometrium with the myometrium (ENDO/MYO) and myometrium (MYO) alone were collected on days 12-14 of the estrous cycle (control group; n = 5) or pregnancy (experimental group; n = 5). Two analogues of LPA at increasing doses were used: oleoyl-sn-glycero-3-phosphate (L-α-LPA, a selective agonist of LPA 1 and LPA 2 receptors; 10 -7 M; 10 -6 M and 10 -5 M) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT, a selective agonist of LPA 3 receptor; 68 nM; 136 nM and 680 nM). L-α-LPA caused an increase in the contraction tension, amplitude and frequency of ENDO/MYO from the uterine horn with the developing embryos. This effect was not observed in MYO in both groups examined. In the ENDO/MYO strips of the uterine horn with developing embryos, OMPT significantly increased the contraction tension at the highest dose (680 nM) and amplitude at all doses examined, while frequency of contractions was decreased at doses of 136 nM and 680 nM. In the MYO strips of the uterine horn with embryos a significant increase in the contraction tension and amplitude after the highest dose of OMPT was observed. The results obtained imply the important role of receptors LPA 1 , LPA 2 and LPA 3 in the contraction activity of the porcine uterus during early pregnancy.

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“…Thackare et al (19) also explained that paracrine action manifests itself in the stimulation of contractility of the tubuli seminiferi, epididymis, and prostate in males. On the other hand, oxytocin administered with a seminal dose stimulated the smooth uterine muscles to action (20). Another study conducted by Langendijk et al (13) demonstrated that intravenous or intramuscular administration of oxytocin during estrus affects the amplitude and frequencies of uterus muscle movements, especially in sows normally characterized by weaker activity of the uterus.…”

Section: Discussionmentioning

confidence: 99%

Effect of oxytocin treatment on the reproductive performance of sows after artificial insemination with liquid semen

Duziński¹,

Knecht²,

Gajewczyk³

2013

Turk J Vet Anim Sci

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Myometrial activity around estrus in sows: spontaneous activity and effects of estrogens, cloprostenol, seminal plasma and clenbuterol

Cited by 45 publications

References 23 publications

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Effect of oxytocin treatment on the reproductive performance of sows after artificial insemination with liquid semen

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