The Red Data Book of Ukraine is an official national Red List of threatened species of plants, animals and fungi that are protected by law in Ukraine. This state document contains information about the current status of these species, threats and measures for their conservation and reproduction. According to the Law of Ukraine On the Red Book Data of Ukraine, the Cabinet of Ministers of Ukraine provides official publication of the printed version of the Red Data Book of Ukraine at least once a decade. In 2021, based on the results of the studies carried out over the past decade, the list of fungi in the Red Data Book of Ukraine was extended to include twenty more species (Amylocystis lapponica, Battarrea phalloides, Butyriboletus appendiculatus, B. fechtneri, B. fuscoroseus, B. subappendiculatus, Chalciporus rubinus, Hemileccinum depilatum, Hericium erinaceum, Kavinia alboviridis, Montagnea radiosa, Mythicomyces corneipes, Phellinidium ferrugineofuscum, Plectania melastoma, Poronia punctata, Rubroboletus lupinus, R. rhodoxanthus, R. rubrosanguineus, R. satanas, and Suillus plorans), while nine species of fungi were excluded for various reasons (Anthurus archeri, Clathrus ruber, Entoloma nidorosum, Gyromitra slonevskii, Laricifomes officinalis, Morchella steppicola, Mutinus ravenelii, Phallus duplicatus, and Pseudocolus fusiformis). The article provides brief information and original photographs of the added twenty species of fungi of national conservation concern.