n-3 and n-6 polyunsaturated fatty acids differentially regulate adipose angiotensinogen and other inflammatory adipokines in part via NF-κB-dependent mechanisms

Siriwardhana, Nalin; Kalupahana, Nishan S.; Fletcher, Sarah; Xin, Wenting; Claycombe, Kate J.; Quignard‐Boulangé, Annie; Zhao, Ling; Saxton, Arnold M.; Moustaid‐Moussa, Naima

doi:10.1016/j.jnutbio.2011.11.009

Cited by 76 publications

(58 citation statements)

References 53 publications

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“…We have previously shown that adipose tissue angiotensiongen II (Agt) and IL-6 were significantly reduced in epididymal adipose tissue in HFD-fed mice supplemented with EPA-enriched fish oil 72. In a set of in vitro experiments, this data was corroborated by downregulation of Agt, IL-6 and MCP-1 production in mouse 3T3-L1 adipocytes treated with EPA.…”

Section: Molecular Mechanisms Of Irmentioning

confidence: 80%

“…In a set of in vitro experiments, this data was corroborated by downregulation of Agt, IL-6 and MCP-1 production in mouse 3T3-L1 adipocytes treated with EPA. Gene regulation by ω−3 PUFA were exerted via reducing NF-κB activation 72. In this regard, miR-146a and miR-222, known for their associations with NF-κB and TNF-α pathways, respectively,73 look particularly promising targets for further investigations.…”

Section: Molecular Mechanisms Of Irmentioning

confidence: 99%

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Adipose Tissue Inflammation in Insulin Resistance: Review of Mechanisms Mediating Anti-Inflammatory Effects of Omega-3 Polyunsaturated Fatty Acids

Pahlavani

Ramalho

Koboziev

et al. 2017

Journal of Investigative Medicine

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Obesity is an increasingly costly and widespread epidemic, effecting 1 in 10 adults worldwide. It has been causally linked with both the metabolic syndrome and insulin resistance, both of which are associated with increased chronic inflammation. The exact mechanisms through which inflammation may contribute to both MetS and IR are numerous and their details are still largely unknown. Recently, micro-RNAs (miRNAs) have emerged as potential interventional targets due to their potential preventive roles in the pathogenesis of several diseases, including MetS and obesity. The purpose of this review paper is to discuss some of the known roles of miRNAs as mediators of inflammation-associated obesity and IR and how omega-3 polyunsaturated fatty acids may be used as a nutritional intervention for these disorders.

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Section: Molecular Mechanisms Of Irmentioning

confidence: 80%

Section: Molecular Mechanisms Of Irmentioning

confidence: 99%

Adipose Tissue Inflammation in Insulin Resistance: Review of Mechanisms Mediating Anti-Inflammatory Effects of Omega-3 Polyunsaturated Fatty Acids

Pahlavani

Ramalho

Koboziev

et al. 2017

Journal of Investigative Medicine

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“…Spontaneously hypertensive rats supplemented with n-3 PUFAs show decreased blood pressure induced by Ang II [38], and EPA supplementation can attenuate angiotensinogen secretion in adipocytes [58]. One of the mechanisms that could explain the effects of fish oil on RAS is through activation of PPARs, especially PPARγ.…”

Section: Discussionmentioning

confidence: 99%

A high-fish-oil diet prevents adiposity and modulates white adipose tissue inflammation pathways in mice

Bargut

Mandarim-de-Lacerda

Águila

2015

The Journal of Nutritional Biochemistry

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“…Without stimulation of the inflammatory pathways (this study), Lyc only suppressed the secretion of the macrophage attracting chemokine MCP-1 [53] from human adipocytes. Furthermore, several studies indicate that treatment of mature SGBS and 3T3-L1 adipocytes with Res (100 μM, 48 h) [30, 54] and EPA (200 μM, 48 h) [55, 56], respectively, and supplementation of HFD-fed mice with both bio-actives (Res, 10 weeks [42]; EPA, 5 or 11 weeks [55, 56]) can reduce the expression and secretion of pro-inflammatory cytokines such as MCP-1, IL-6 and IL-8 in AT.…”

Section: Discussionmentioning

confidence: 99%

Combinations of bio-active dietary constituents affect human white adipocyte function in-vitro

Warnke¹,

Jocken

Schoop³

et al. 2016

Nutr Metab (Lond)

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BackgroundSpecific bio-active dietary compounds modulate numerous metabolic processes in adipose tissue (AT), including pre-adipocyte proliferation and differentiation. AT dysfunction, rather than an increased fat mass per se, is strongly associated with the development of insulin resistance and is characterized by impaired adipogenesis, hypertrophic adipocytes, inflammation, and impairments in substrate metabolism. A better understanding of mechanisms underlying AT dysfunction may provide new strategies for the treatment of obesity-associated metabolic diseases. Here we evaluated the role of (all-E)-lycopene (Lyc), eicosapentaenoic acid (EPA) or trans-resveratrol (Res) and combinations thereof on human white adipocyte function.MethodsIn-vitro differentiating human pre-adipocytes were treated with EPA, Lyc and Res or their combinations for 14 days. The effects on intracellular lipid droplet (LD) accumulation, secreted anti- and pro-inflammatory cyto-/adipokines (e.g. adiponectin, IL-6, IL-8/CXCL-8 and MCP-1/CCL2) and on gene expression of markers of adipocyte differentiation and substrate metabolism (e.g. PPAR-gamma, C/EBP-alpha, GLUT-4, FAS, ATGL, HSL, and PLIN-1) were measured by fluorescent microscopy (Cellomics™), multi-parametric LiquiChip® technology and quantitative RT-PCR, respectively.ResultsTreatment of differentiating adipocytes for 14 days with the combination of Lyc/Res and EPA/Res resulted in significantly inhibited LD formation (~ -25 and -20%, respectively) compared to the effects of the single compounds. These morphological changes were accompanied by increased mRNA levels of the adipogenic marker PPAR-gamma and the lipase ATGL and by decreased expression levels of lipogenic markers (LPL, FAS, GLUT-4) and the LD-covering protein PLIN-1. In addition, a blunted adipocyte secretion of pro-inflammatory cytokines (IL-6 and MCP-1) and adiponectin was observed following treatment with these compounds.ConclusionThe combination of the dietary bio-actives Lyc and EPA with Res might influence adipocyte function by affecting the balance between adipogenic, lipogenic and lipolytic gene expression, resulting in a reduced LD storage and a less inflammatory secretion profile. Taken together, our results indicate that combinations of dietary compounds may be beneficial for the prevention and treatment of metabolic disorders via effects on human white adipocyte function.Electronic supplementary materialThe online version of this article (doi:10.1186/s12986-016-0143-5) contains supplementary material, which is available to authorized users.

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n-3 and n-6 polyunsaturated fatty acids differentially regulate adipose angiotensinogen and other inflammatory adipokines in part via NF-κB-dependent mechanisms

Cited by 76 publications

References 53 publications

Adipose Tissue Inflammation in Insulin Resistance: Review of Mechanisms Mediating Anti-Inflammatory Effects of Omega-3 Polyunsaturated Fatty Acids

Adipose Tissue Inflammation in Insulin Resistance: Review of Mechanisms Mediating Anti-Inflammatory Effects of Omega-3 Polyunsaturated Fatty Acids

A high-fish-oil diet prevents adiposity and modulates white adipose tissue inflammation pathways in mice

Combinations of bio-active dietary constituents affect human white adipocyte function in-vitro

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