N-3 PUFA increase bioavailability and function of endothelial progenitor cells

Spigoni, Valentina; Lombardi, Carlo; Cito, Monia; Picconi, Angela; Ridolfi, Valentina; Andreoli, Roberta; Anelli, Nadia; Gnudi, Luigi; Goldoni, Matteo; Zavaroni, Ivana; Raddino, Riccardo; Dei, Alessandra

doi:10.1039/c3fo60641d

Cited by 27 publications

(19 citation statements)

References 42 publications

(49 reference statements)

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“…EPC levels were assessed by cytofluorimetric analysis and identified as cells co-expressing CD34, CD133, and Kinase insert Domain Receptor (KDR) surface antigens as previously described [ 42 , 43 , 44 , 45 ]. Briefly, 300 µL of blood collected in EDTA were stained with 15 μL fluorescein isothiocyanate (FITC)-conjugated anti-human CD34 monoclonal antibody (mAb) (Becton Dickinson Biosciences, Franklin Lakes, NJ, USA), 20 μL phycoerythrin (PE)-conjugated anti-human KDR mAb (R&D Systems, Minneapolis, MN, USA) and 20 µL allophycocyanin (APC)-conjugated anti-human CD133 mAb (Miltenyi Biotec, San Diego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Effects of a New Nutraceutical Formulation (Berberine, Red Yeast Rice and Chitosan) on Non-HDL Cholesterol Levels in Individuals with Dyslipidemia: Results from a Randomized, Double Blind, Placebo-Controlled Study

Spigoni

Aldigeri

Antonini

et al. 2017

IJMS

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Increased non high-density lipoprotein (HDL)/low-density lipoprotein (LDL) cholesterol levels are independent risk factors for cardiovascular (CV) mortality with no documented threshold. A new combination of nutraceuticals (berberine 200 mg, monacolin K 3 mg, chitosan 10 mg and coenzyme Q 10 mg) with additive lipid-lowering properties has become available. The aim of the study is to test the efficacy of the nutraceutical formulation (one daily) in lowering non-HDL cholesterol vs. placebo at 12 weeks in individuals with non-HDL-cholesterol levels ≥160 mg/dL. 39 subjects (age 52 ± 11 years; 54% females; body mass index 27 ± 4 kg/m2) were randomized (3:1) in a double blind phase II placebo-controlled study. At baseline, 4 and 12 weeks main clinical/biohumoral parameters, pro-inflammatory cytokines, (gut)-hormones, proprotein convertase subtilisin/kexin type 9 (PCSK9) levels and endothelial progenitor cell (EPC) number were assessed. Baseline characteristics were comparable in the two groups. The intervention significantly decreased non-HDL cholesterol (−30 ± 20 mg/dL; p = 0.012), LDL cholesterol (−31 ± 18 mg/dL, p = 0.011) and apolipoprotein (Apo) B (−14 ± 12 mg/dL, p = 0.030) levels compared to the placebo. Pro-inflammatory, hormonal, PCSK9 and EPC levels remained stable throughout the study in both groups. The intervention was well tolerated. Three adverse events occurred: Epstein Barr virus infection, duodenitis and asymptomatic but significant increase in creatine phosphokinase (following intense physical exercise) which required hospitalization. The tested nutraceutical formulation may represent a possible therapeutic strategy in dyslipidemic individuals in primary prevention.

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Section: Methodsmentioning

confidence: 99%

Effects of a New Nutraceutical Formulation (Berberine, Red Yeast Rice and Chitosan) on Non-HDL Cholesterol Levels in Individuals with Dyslipidemia: Results from a Randomized, Double Blind, Placebo-Controlled Study

Spigoni

Aldigeri

Antonini

et al. 2017

IJMS

Self Cite

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“…26 Angiogenin can also promote EPC proliferation and colony formation. 27 In terms of the downregulated cytokines, IL-8, 28 MCP-1 29 and MIP-1β 30 are pro-inflammatory factors and can exert adverse effects on EPC or endothelial cell biology. Moreover, TNF-α can impair EPC proliferation, migration, adhesion capacity, and vasculogenesis.…”

Section: Discussionmentioning

confidence: 99%

Transplantation of Endothelial Progenitor Cells Overexpressing miR-126-3p Improves Heart Function in Ischemic Cardiomyopathy

Liu

Wang

et al. 2018

Circ J

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Background: In a previous study, a low level of miR-126-3p in endothelial progenitor cells (EPCs) was linked to the outcome of ischemic cardiomyopathy (ICM) patients. However, it remains unclear whether transplantation with miR-126-3p-overexpressing EPCs (MO-EPCs) can improve the cardiac function of ICM animal models.Methods and Results: miR-126-3p overexpression by lentiviral vector significantly increased migration and tube-like structures of EPCs from ICM patients. MO-EPCs or non-modified EPCs (NM-EPCs) were transplanted into nude rats with ICM induced by coronary artery ligation. MO-EPC transplantation increased capillary density and EPC survival rate in myocardial tissues of nude rats. Cytokines were also assessed by antibody array and real-time RT-PCR. G-CSF, VEGF-A, IL-3, IL-10, IGF-1, angiogenin, HGF, TIMP-1 and TIMP-2 were upregulated, and IL-8, MCP-1, MCP-2, TNF-α, TNF-β and MIP-1β were downregulated after miR-126-3p overexpression in EPCs. The same results were obtained in infarction tissues of nude rats after MO-EPC transplantation. Eight weeks after MO-EPC transplantation, left ventricular function improved significantly with clearly decreased infarction size, increased anterior wall thickness, and inhibition of inflammation compared with the results for NM-EPC transplantation. However, MO-EPC transplantation showed no increase in survival time of nude rats with ICM during 8 weeks of observation.Conclusions: miR-126-3p can restore the biology of EPCs from ICM patients. Moreover, MO-EPC transplantation improves cardiac function effectively, representing a promising future treatment for ICM.

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“…2 For example, research developed by our group showed that the echinoderm Marthasterias glacialis (spiny sea-star) contains a big amount of fatty acids, being an interesting source of anti-inammatory molecules. 5,6 As vegetable oils do not contain long-chain PUFA and the conversion of C 18 PUFA to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the human body is inefficient, there has been considerable interest in nding new marine sources for these fatty acids. 4 The u-3 PUFA provide a myriad of health benets, including reduction of cardiovascular diseases and anti-carcinogenic activities.…”

Section: Introductionmentioning

confidence: 99%

Fatty acids from edible sea hares: anti-inflammatory capacity in LPS-stimulated RAW 264.7 cells involves iNOS modulation

et al. 2015

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The inclusion of marine organisms in the diet is gaining importance due to their richness in health beneficial nutrients. This study evaluated the fatty acid composition and anti-inflammatory potential of lipophilic extracts of two edible sea hares, Aplysia fasciata Poiret and Aplysia punctata Cuvier. Twenty-five fatty acids were determined, nine of them not yet reported in these species. They revealed similar antiinflammatory properties in RAW 264.7 cells stimulated with lipopolysaccharide, as ascertained by the decreased levels of cNO in the culture medium. A decrease of L-citrulline was also observed, indicating that the compounds may act by modulation of inducible nitric oxide synthase (iNOS). A. punctata was most effective as lipoxygenase inhibitor, probably because it contains more polyunsaturated fatty acids (PUFA) that can compete with linoleic acid for the active site, decreasing enzyme activity. The antiinflammatory potential of A. fasciata and A. punctata is reported here for the first time.

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N-3 PUFA increase bioavailability and function of endothelial progenitor cells

Abstract: Our findings support the case of a beneficiary role played by n-3 PUFA in EPC function and bioavailability.

Cited by 27 publications

References 42 publications

Effects of a New Nutraceutical Formulation (Berberine, Red Yeast Rice and Chitosan) on Non-HDL Cholesterol Levels in Individuals with Dyslipidemia: Results from a Randomized, Double Blind, Placebo-Controlled Study

Effects of a New Nutraceutical Formulation (Berberine, Red Yeast Rice and Chitosan) on Non-HDL Cholesterol Levels in Individuals with Dyslipidemia: Results from a Randomized, Double Blind, Placebo-Controlled Study

Transplantation of Endothelial Progenitor Cells Overexpressing miR-126-3p Improves Heart Function in Ischemic Cardiomyopathy

Fatty acids from edible sea hares: anti-inflammatory capacity in LPS-stimulated RAW 264.7 cells involves iNOS modulation

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