N-CAM expression and localization in PC12 cells modulated by extracellular peptides

Mariggiò, Maria Addolorata; Guarnieri, Simone; Mariggiò, Stefania; Gianfranceschi, G.; Fanò, Giorgio

doi:10.1016/s0196-9781(02)00258-9

Cited by 8 publications

(2 citation statements)

References 30 publications

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“…After incubation for 30 min at 37 °C, the plates were read at 560 nm in a spectrophotometer (Spectramax SM190). The cell numbers were determined from the optical density values obtained using a standard curve [38].…”

Section: Mtt Assaymentioning

confidence: 99%

Calcitonin-Induced Effects on Amniotic Fluid-Derived Mesenchymal Stem Cells

Morabito

D’Alimonte²,

Pierdomenico³

et al. 2015

Cell Physiol Biochem

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Background/Aims: Mesenchymal stem cells from human amniotic fluid (huAFMSCs) can differentiate into multiple lineages and are not tumorigenic after transplantation, making them good candidates for therapeutic purposes. The aim was to determine the effects of calcitonin on these huAFMSCs during osteogenic differentiation, in terms of the physiological role of calcitonin in bone homeostasis. Methods: For huAFMSCs cultured under different conditions, we assayed: expression of the calcitonin receptor, using immunolabelling techniques; proliferation and osteogenesis, using colorimetric and enzymatic assays; intracellular Ca²⁺ and cAMP levels, using videomicroscopy and spectrophotometry. Results: The calcitonin receptor was expressed in proliferating and osteo-differentiated huAFMSCs. Calcitonin triggered intracellular Ca²⁺ increases and cAMP production. Its presence in cell medium also induced dose-dependent inhibitory effects on proliferation and increased osteogenic differentiation of huAFMSCs, as also indicated by enhancement of specific markers and alkaline phosphatase activity. Conclusions: These data show that huAFMSCs represent a potential osteogenic model to study in-vitro cell responses to calcitonin (and other members of the calcitonin family). This leads the way to the opening of new lines of research that will add new insight both in cell therapies and in the pharmacological use of these molecules.

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Section: Mtt Assaymentioning

confidence: 99%

Calcitonin-Induced Effects on Amniotic Fluid-Derived Mesenchymal Stem Cells

Morabito

D’Alimonte²,

Pierdomenico³

et al. 2015

Cell Physiol Biochem

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“…Recently, they have been found in animal synaptosomes where they appear to play a role in intercellular information transfer. 65,66…”

Section: Chalones a Facet Of A General Intercellular Signal Network?mentioning

confidence: 99%

Chalones – From Aqueous Extracts To Oligopeptides

Elgjo¹,

Reichelt²

2004

Cell Cycle

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The term "chalone" was coined about 40 years ago to describe endogenous growthinhibiting factors with a tissue-specific and reversible effect. A large number of "chalones" were reported in the 1970's and early 80's. The term was, however, used rather indiscriminately and attempts at purification of the active component(s) were without success. As a result, most laboratories lost interest. Then, in the early 1980's, two different research teams demonstrated that the active growth-inhibiting constituents were N-substituted oligopeptides. In the following years, similar oligopeptides were characterized in extracts of liver, the large bowel, melanocytes, lymphocytes, and neuroblastoma, in addition to the two first ones from monomyelopoietic cells and epidermis, respectively. All peptides have a bell-shaped dose response pattern and optimal effect at very low concentrations. Several of them inhibit growth even in tumors derived from the respective organs. Preliminary studies have revealed that two of the oligopeptides alter the expression of growth-and differentiation-related genes. Quite recently, some papers are again using the term "chalone" and a short review therefore seems appropriate.

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