N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging

Bhattarai, Shanta Raj; Kc, Remant Bahadur; Kim, Sun Y.; Sharma, Manju; Khil, Myung-Seob; Hwang, Pyoung Han; Chung, Gyung Ho; Kim, Hak Y

doi:10.1186/1477-3155-6-1

Cited by 74 publications

(32 citation statements)

References 13 publications

(17 reference statements)

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“…After 6 hours, 200 mg of pluronic polymer F68 (average molecular weight 8350) in 5 mL water was added to the nanoparticle suspension and stirred overnight. After five washes with water, 33 the nanoparticles were resuspended in 25 mL water and centrifuged at 1000 rpm to remove larger aggregates. This formulation was designated as MNP.…”

Section: Preparation Of Mnp and Mnp-cur Formulationsmentioning

confidence: 99%

“…The drug-loaded nanoparticles were washed three times by resuspending them in water and separated with the help of magnets. 33 These drug-loaded nanoparticles were dispersed in 2 mL sterile phosphate buffered saline (PBS) solution and stored in a refrigerator until further use. This formulation was designated as MNP-CUR.…”

Section: Preparation Of Mnp and Mnp-cur Formulationsmentioning

confidence: 99%

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Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Yallapu¹,

Othman²,

Curtis³

et al. 2012

IJN

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Background:The next generation magnetic nanoparticles (MNPs) with theranostic applications have attracted significant attention and will greatly improve nanomedicine in cancer therapeutics. Such novel MNP formulations must have ultra-low particle size, high inherent magnetic properties, effective imaging, drug targeting, and drug delivery properties. To achieve these characteristic properties, a curcumin-loaded MNP (MNP-CUR) formulation was developed. Methods: MNPs were prepared by chemical precipitation method and loaded with curcumin (CUR) using diffusion method. The physicochemical properties of MNP-CUR were characterized using dynamic light scattering, transmission electron microscopy, and spectroscopy. The internalization of MNP-CUR was achieved after 6 hours incubation with MDA-MB-231 breast cancer cells. The anticancer potential was evaluated by a tetrazolium-based dye and colony formation assays. Further, to prove MNP-CUR results in superior therapeutic effects over CUR, the mitochondrial membrane potential integrity and reactive oxygen species generation were determined. Magnetic resonance imaging capability and magnetic targeting property were also evaluated. Results: MNP-CUR exhibited individual particle grain size of ∼9 nm and hydrodynamic average aggregative particle size of ∼123 nm. Internalized MNP-CUR showed a preferential uptake in MDA-MB-231 cells in a concentration-dependent manner and demonstrated accumulation throughout the cell, which indicates that particles are not attached on the cell surface but internalized through endocytosis. MNP-CUR displayed strong anticancer properties compared to free CUR. MNP-CUR also amplified loss of potential integrity and generation of reactive oxygen species upon treatment compared to free CUR. Furthermore, MNP-CUR exhibited superior magnetic resonance imaging characteristics and significantly increased the targeting capability of CUR. Conclusion: MNP-CUR exhibits potent anticancer activity along with imaging and magnetic targeting capabilities. This approach can be extended to preclinical and clinical use and may have importance in cancer treatment and cancer imaging in the future. Further, if these nanoparticles can functionalize with antibody/ligands, they will serve as novel platforms for multiple biomedical applications.

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Section: Preparation Of Mnp and Mnp-cur Formulationsmentioning

confidence: 99%

Section: Preparation Of Mnp and Mnp-cur Formulationsmentioning

confidence: 99%

Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Yallapu¹,

Othman²,

Curtis³

et al. 2012

IJN

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“…However, there are not many reports on detailed studies of the growth process of monodispersed superparamagnetic iron oxide nanoparticles (SPIONs), although the syntheses of relatively uniform magnetite and maghemite nanoparticles have been reported. 8 It was observed that the wet chemical method for iron oxide nanoparticles is advantageous since it was a method to produce under 10 nm particles.…”

Section: Introductionmentioning

confidence: 99%

Investigation and Characterization of Maghemite (γ-Fe2O3) Nanoparticles and Its Cytotoxicity Studies

Khodabakhshi¹,

Bahari²

2017

IJPER

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Objective: The expansion of wet chemical method for the synthesis of iron oxide nanoparticles with magnetic properties is reported. Methods: Iron oxide nanoparticles were investigated in the procedure using Transmission Electron Microscopy (TEM). It has been seen that the distribution size changed by changing of Molarity precursor and heating the sample. The magnetization evaluations were performed using a Vibrating Sample Magnetometer device (VSM) to specify the magnetic answer. The Thermo Gravimetric Analyses (TGA) displayed the size-dependent weight loss of the magnetic nanoparticles. In this work, the cytotoxic activity of various samples of Maghemite on cancer cell lines HELA was studied. The in vitro cytotoxicity tests were also performed to determine the cell viability as a work of size and heat of the magnetic nanoparticles. Results: The samples c and d at T=300°C had a little magnetic hysteresis that can use in cancer cure with hyperthermia therapy method. Conclusions: These samples became amorphous and they were nontoxicity.

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“…where the shift is due to the addition of vibration from O -H [24]. Based on the FTIR data, in combination with the elemental analysis, we believed that the most likely component of the nanoparticles was glycoprotein, owing to the shared amide II band with the BSA spectrum, and the shared CO-C band with the chitosan spectrum.…”

Section: Chemical Analysis Of the Ivy Nanoparticlesmentioning

confidence: 94%

Isolation and chemical analysis of nanoparticles from English ivy ( Hedera helix L.)

Lenaghan

Burris²,

Chourey

et al. 2013

J. R. Soc. Interface.

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Bio-inspiration for novel adhesive development has drawn increasing interest in recent years with the discovery of the nanoscale morphology of the gecko footpad and mussel adhesive proteins. Similar to these animal systems, it was discovered that English ivy (Hedera helix L.) secretes a high strength adhesive containing uniform nanoparticles. Recent studies have demonstrated that the ivy nanoparticles not only contribute to the high strength of this adhesive, but also have ultraviolet (UV) protective abilities, making them ideal for sunscreen and cosmetic fillers, and may be used as nanocarriers for drug delivery. To make these applications a reality, the chemical nature of the ivy nanoparticles must be elucidated. In the current work, a method was developed to harvest bulk ivy nanoparticles from an adventitious root culture system, and the chemical composition of the nanoparticles was analysed. UV/ visible spectroscopy, inductively coupled plasma mass spectrometry, Fourier transform infrared spectroscopy and electrophoresis were used in this study to identify the chemical nature of the ivy nanoparticles. Based on this analysis, we conclude that the ivy nanoparticles are proteinaceous.

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N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging

Cited by 74 publications

References 13 publications

Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Investigation and Characterization of Maghemite (γ-Fe2O3) Nanoparticles and Its Cytotoxicity Studies

Isolation and chemical analysis of nanoparticles from English ivy ( Hedera helix L.)

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