N<sub>2</sub>O emission and bacterial community dynamics during realization of the partial nitrification process

Liu, Xiaolin; Ni, Shou-Qing; Guo, Wenshan; Wang, Zhibin; Ahmad, Hafiz Adeel; Gao, Baoyu; Xu, Fang

doi:10.1039/c8ra03032d

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…The next-generation sequencing was applied to determine the microbial community structure based on the universal primer pair of 16S rDNA 28 . The V4 amplicons were sequenced using primers 515F (GTGCCAGCMGCCGCGGTA) and 806R (GGACTACNVGGGTWTCTAAT) and the pair-ended method on the Illumina Miseq sequencing platform at Personal Biotechnology Co., Ltd. 29 .…”

Section: Methodsmentioning

confidence: 99%

Insight into impact of sewage discharge on microbial dynamics and pathogenicity in river ecosystem

Xie

Liu

Wei

et al. 2022

Sci Rep

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Direct sewage discharge could cause copious numbers of serious and irreversible harm to the environment. This study investigated the impacts of treated and raw sewage on the river ecosystem. Through our analysis, sewage carried various nutrients into the river, leading to changes in the microbial community in the river and reducing the diversity and richness of bacteria. The relative abundances of Hydrogenophaga, Thauera, Planctomyces, Zoogloea, and Pseudomonas boosted from 0.25, 0.01, 0.00, 0.05, and 0.08% to 3.33, 3.43, 0.02, 6.28, and 2.69%, before and after raw sewage discharge, respectively. The gene abundance of pathogenic bacteria significantly increased after raw sewage discharge. For instance, the gene abundance of Vibrio, Helicobacter, Tuberculosis, and Staphylococcus augmented from 4055, 3797, 13,545, 33 reads at Site-1 to 23,556, 13,163, 19,887, 734 reads at Site-2, respectively. In addition, according to the redundancy analysis (RDA), the infectious pathogens were positively related to the environmental parameters, in which COD showed the highest positive correlation with Mycobacterium tuberculosis. Additionally, river self-purification may contribute to improving water quality and reducing pathogenicity. The outcomes of this study showed that direct discharge brought pathogens and changed microbial community structure of the river.

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Section: Methodsmentioning

confidence: 99%

Insight into impact of sewage discharge on microbial dynamics and pathogenicity in river ecosystem

Xie

Liu

Wei

et al. 2022

Sci Rep

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show abstract

“…The next-generation sequencing was applied to determine the microbial community structure based on the universal primer pair of 16S rDNA (Liu et al, 2018). The V4 amplicons were sequenced using primers 515F (GTGCCAGCMGCCGCGGTA) and 806R (GGACTACNVGGGTWTCTAAT) and the pair-ended method by Illumina Miseq sequencing platform (Lv et al, 2016).…”

Section: High-throughput 16s Rdna Sequencingmentioning

confidence: 99%

Insight Into Impacts of Sewage Discharge and River Self-Purification on Microbial Dynamics and Pathogenicity in River Ecosystem

Xie¹,

Liu²,

Wei³

et al. 2021

Preprint

Self Cite

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Sewage directly discharge causes serious environmental problems. Here, the effects of treated and untreated sewage on the river ecosystem were investigated. The variations of microbial community structure, including infectious pathogenic bacteria and functional bacteria related to nitrogen, phosphorus, and COD metabolism were studied in detail. Bacterial diversity and richness were significantly decreased, while, Proteobacteria containing various infectious pathogens, such as Vibrio and Helicobacter, significantly increased after the discharge of raw sewage. Although the microbial structure was slightly restored and the abundance of most pathogenic bacteria was also slightly reduced through river self-purification, direct discharge of raw sewage caused severe and short-term irreversible damage to the river environment. Direct discharge also introduced various pollutants such as nitrogen, phosphorus, and COD, increasing the corresponding functional bacteria and their related genes. Furthermore, the high abundance of pathogenic bacteria of the drain outlet was mainly from raw sewage rather than bacteria reproduction caused by water deterioration according to the RDA analysis. With these results, direct discharge disturbed the ecological balance of the river. Therefore, more attention is needed to provide a hygienic situation for people and all sewage should be treated properly. In conclusion, all sewage should be treated properly before discharge into ecosystems to mitigate its negative impacts on receiving water bodies.

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“…It is known that the key to achieving stable partial nitrication is to sustainably retain AOB while eliminating NOB in the mainstream BNR process. 30,31 However, raw sewage that contains a few nitriers, especially the more efficient NOB in seeding as shown in Table 3, continuously inoculates the bioreactor, which might have a devastating effect on achieving stable partial nitrication. Duan et al reported that the continuously seeding of NOB in raw sewage resulted in different extents of ineffective NOB suppression in the mainstream activated sludge systems.…”

Section: The Intractable Challenge For Achieving Partial Nitrication In the Mainstreammentioning

confidence: 99%

The differential proliferation of AOB and NOB during natural nitrifier cultivation and acclimation with raw sewage as seed sludge

Wang

et al. 2020

RSC Adv.

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N₂O emission and bacterial community dynamics during realization of the partial nitrification process

Abstract: In this study, greenhouse gas emissions and microbial community succession during the realization of the partial nitrification (PN) process were studied.

Cited by 5 publications

References 35 publications

Insight into impact of sewage discharge on microbial dynamics and pathogenicity in river ecosystem

Insight into impact of sewage discharge on microbial dynamics and pathogenicity in river ecosystem

Insight Into Impacts of Sewage Discharge and River Self-Purification on Microbial Dynamics and Pathogenicity in River Ecosystem

The differential proliferation of AOB and NOB during natural nitrifier cultivation and acclimation with raw sewage as seed sludge

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N2O emission and bacterial community dynamics during realization of the partial nitrification process

Abstract: In this study, greenhouse gas emissions and microbial community succession during the realization of the partial nitrification (PN) process were studied.

Cited by 5 publications

References 35 publications

Insight into impact of sewage discharge on microbial dynamics and pathogenicity in river ecosystem

Insight into impact of sewage discharge on microbial dynamics and pathogenicity in river ecosystem

Insight Into Impacts of Sewage Discharge and River Self-Purification on Microbial Dynamics and Pathogenicity in River Ecosystem

The differential proliferation of AOB and NOB during natural nitrifier cultivation and acclimation with raw sewage as seed sludge

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Product

Resources

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N₂O emission and bacterial community dynamics during realization of the partial nitrification process