Na-K-ATPase generates an active transport potential in the gills of the hyperregulating shore crab Carcinus maenas

Siebers, D.; Winkler, Andreas; Lucu, Čedomil; Thedens, G.; Weichart, D.

doi:10.1007/bf00539427

Cited by 100 publications

(51 citation statements)

References 12 publications

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“…Consequently, the comparatively small active components that are measurable only under symmetrical conditions were overlooked. When isolated crab gills were -as in the present experiments -bathed and perfused with the same solution symmetrically on both sides, a spontaneous transepithelial potential difference between bath side (external medium) and blood side (internal medium) was established (Drews, 1985;Siebers et al, 1985Siebers et al, , 1986.…”

Section: Whole Body Potentialsmentioning

confidence: 99%

“…It was suggested by the work of Siebers et al (1985) that the Na-K-ATPase is responsible for the establishment of the transepithelial potential difference at the gill level.…”

Section: Whole Body Potentialsmentioning

confidence: 99%

“…It is shown in the present work that relatively large negative PDs of similar millivolts can be measured between blood and medium. They suggestedly (Siebers et al, 1985 represent the driving force for active Na uptake. In addition, these PDs depend on the salinity of the incubation medium.…”

Section: Whole Body Potentialsmentioning

confidence: 99%

“…The technique of perfusing isolated gills of Carcinus maenas was applied according to Siebers et al (1985). Measurements of potential differences were performed by means of two electrodes (Ag/AgC1 in 3 M KC1, Ingold Frankfurt/Main) connected with the internal and external medium via agar bridges (3 % agar in 3 M KCL).…”

Section: Potential Differences Of Isolated Perfused Gillsmentioning

confidence: 99%

“…In this context, Siebers et aL (1985) have shown that the Na-K-ATPase generates a transepithelial potential difference (PD) between external medium and blood in the hyperregulating shore crab Carcinus maenas. The advantage of this method is that the potential difference between blood and medium is stable during several hours of the experiments.…”

Section: Introductionmentioning

confidence: 99%

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The role of the transbranchial potential difference in hyperosmotic regulation of the shore crabCarcinus maenas

Winkler

1986

Helgolander Meeresunters

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When isolated gills of the shore crab Carcinus maenas were bathed and perfused with identical solutions on both sides (50 % sea water), a spontaneous transepithelial potential difference (PD) of some millivolts (hemolymph side negative) was established. This PD is of active nature and requires the metabolism of the living cell, since it uses its own sources of energy in addition to organic nutrients offered in the flow of artificial hemolymph. Addition of sodium cyanide and dinitrophenole to bathing and perfusion medium resulted in reversible breakdown of PDs in a concentration-dependent mode. In posterior gills of C. maenas, the potential differences were more negative compared to data measured in anterior gills of the same individuals. These results are correlated with higher specific activities of Na-K-ATPase in posterior gills. Experiments with triamterene indicate that sodium uptake in C. maenas is sensitive to this diuretic drug, when applied on the apical side of the epithelial cell. The results obtained show that active uptake of sodium from medium to blood across the g~s is performed by a complex mechanism including participation of several basal and apical transport steps.

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Section: Whole Body Potentialsmentioning

confidence: 99%

“…It was suggested by the work of Siebers et al (1985) that the Na-K-ATPase is responsible for the establishment of the transepithelial potential difference at the gill level.…”

Section: Whole Body Potentialsmentioning

confidence: 99%

Section: Whole Body Potentialsmentioning

confidence: 99%

Section: Potential Differences Of Isolated Perfused Gillsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The role of the transbranchial potential difference in hyperosmotic regulation of the shore crabCarcinus maenas

Winkler

1986

Helgolander Meeresunters

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Osmoregulation and Excretion

Larsen

Deaton

Onken

et al. 2014

Comprehensive Physiology

185

141

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The article discusses advances in osmoregulation and excretion with emphasis on how multicellular animals in different osmotic environments regulate their milieu intérieur. Mechanisms of energy transformations in animal osmoregulation are dealt with in biophysical terms with respect to water and ion exchange across biological membranes and coupling of ion and water fluxes across epithelia. The discussion of functions is based on a comparative approach analyzing mechanisms that have evolved in different taxonomic groups at biochemical, cellular and tissue levels and their integration in maintaining whole body water and ion homeostasis. The focus is on recent studies of adaptations and newly discovered mechanisms of acclimatization during transitions of animals between different osmotic environments. Special attention is paid to hypotheses about the diversity of cellular organization of osmoregulatory and excretory organs such as glomerular kidneys, antennal glands, Malpighian tubules and insect gut, gills, integument and intestine, with accounts on experimental approaches and methods applied in the studies. It is demonstrated how knowledge in these areas of comparative physiology has expanded considerably during the last two decades, bridging seminal classical works with studies based on new approaches at all levels of anatomical and functional organization. A number of as yet partially unanswered questions are emphasized, some of which are about how water and solute exchange mechanisms at lower levels are integrated for regulating whole body extracellular water volume and ion homeostasis of animals in their natural habitats. © 2014 American Physiological Society.

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Basolateral localization of Na⁺ + K⁺‐ATPase in gill epithelium of two osmoregulating crabs, Callinectes sapidus and Carcinus maenas

1986

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Although Na+ + K+‐dependent ATPase, the enzymatic equivalent of the sodium pump, is thought to be restricted to the basolateral membrane of nearly all ion‐transporting epithelial cells, its absence from basolateral membranes of crustacean epithelia and/or its presence in apical membranes have been suggested by evidence from other laboratories. However, ultracytochemical localization of K+‐dependent, ouabain‐sensitive p‐nitrophenylphosphatase (p‐NPPase), using the method of Mayahara et al. ('80), indicated that Na+ + K+‐ATPase resides predominantly in the basolateral membrane of thick epithelial cells from gill lamellae of two euryhaline crabs, Callinectes sapidus and Carcinus maenas. Crab gill is therefore not unique in its Na+ + K+‐ATPase distribution and may be used as a model for experiments designed to investigate mechanisms of transepithelial Na+ uptake.

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Na-K-ATPase generates an active transport potential in the gills of the hyperregulating shore crab Carcinus maenas

Cited by 100 publications

References 12 publications

The role of the transbranchial potential difference in hyperosmotic regulation of the shore crabCarcinus maenas

The role of the transbranchial potential difference in hyperosmotic regulation of the shore crabCarcinus maenas

Osmoregulation and Excretion

Basolateral localization of Na⁺ + K⁺‐ATPase in gill epithelium of two osmoregulating crabs, Callinectes sapidus and Carcinus maenas

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Na-K-ATPase generates an active transport potential in the gills of the hyperregulating shore crab Carcinus maenas

Cited by 100 publications

References 12 publications

The role of the transbranchial potential difference in hyperosmotic regulation of the shore crabCarcinus maenas

The role of the transbranchial potential difference in hyperosmotic regulation of the shore crabCarcinus maenas

Osmoregulation and Excretion

Basolateral localization of Na+ + K+‐ATPase in gill epithelium of two osmoregulating crabs, Callinectes sapidus and Carcinus maenas

Contact Info

Product

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About

Basolateral localization of Na⁺ + K⁺‐ATPase in gill epithelium of two osmoregulating crabs, Callinectes sapidus and Carcinus maenas