Nannochloropsis production metrics in a scalable outdoor photobioreactor for commercial applications

Quinn, Jason C.; Yates, Tracy; Douglas, Nathaniel; Weyer, Kristina M.; Butler, J. N.; Bradley, Thomas H.; Lammers, Peter J.

doi:10.1016/j.biortech.2012.04.073

Cited by 128 publications

(95 citation statements)

References 32 publications

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“…Nannochloropsis cells reproduce asexually, dividing to yield two daughter cells that then shed their mother cell wall (2,3). Several Nannochloropsis species have been studied as candidate production strains in large-scale biofuel facilities because of their hardy outdoor growth profiles and high lipid yields (4)(5)(6)(7)(8)(9). They are also producers of valuable pigments (10) and nutritive oils (11,12) and are commonly used as an aquaculture feed (13).…”

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confidence: 99%

Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall

et al. 2014

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e Marine algae of the genus Nannochloropsis are promising producers of biofuel precursors and nutraceuticals and are also harvested commercially for aquaculture feed. We have used quick-freeze, deep-etch electron microscopy, Fourier transform infrared spectroscopy, and carbohydrate analyses to characterize the architecture of the Nannochloropsis gaditana (strain CCMP 526) cell wall, whose recalcitrance presents a significant barrier to biocommodity extraction. The data indicate a bilayer structure consisting of a cellulosic inner wall (ϳ75% of the mass balance) protected by an outer hydrophobic algaenan layer. Cellulase treatment of walls purified after cell lysis generates highly enriched algaenan preparations without using the harsh chemical treatments typically used in algaenan isolation and characterization. Nannochloropsis algaenan was determined to comprise long, straight-chain, saturated aliphatics with ether cross-links, which closely resembles the cutan of vascular plants. Chemical identification of >85% of the isolated cell wall mass is detailed, and genome analysis is used to identify candidate biosynthetic enzymes.

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confidence: 99%

Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall

et al. 2014

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“…This lipid content was much higher than most of the records in the outdoor cultures, indicating a lipid content of 15.6% for Botryococcus braunii (Bazaes et al, 2012), 19-32% for Chlorella sp (Moheimani, 2012(Moheimani, , 2013, 34.7% for N. oculata and N. salina (Quinn et al, 2012), less than 30% for S. acutus (Doria et al, 2011), and 24-30% for Tetraselmis suecica (Moheimani, 2013). In addition, the lipid productivity averaged at 28.1 mg…”

Section: Abundansmentioning

confidence: 74%

“…And this value was two times higher against 10.7 m 3 ha À1 yr À1 achieved by Nannochloropsis (Quinn et al, 2012). Regarding the biomass productivity and the biomass balance estimation, it can be predicted that the strain S. obtusus XJ-15 has the capability to produce 10,600 L ha À1 yr À1 of biodiesel.…”

Section: Abundansmentioning

confidence: 88%

Selection of microalgae for biodiesel production in a scalable outdoor photobioreactor in north China

Xia

Song

et al. 2014

Bioresource Technology

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Section: Introductionmentioning

confidence: 99%

“…These factors make these strains some of the best lipid producers among 30 marine and freshwater microalgae in terms of both lipid content and lipid productivity (Rodolfi et al 2009). At present, Nannochloropsis have been successfully grown in indoor and outdoor systems for biodiesel or biomass production, as feeding source for rotifers and for creating a 'green-water effect' in fish larvae tanks (Rodolfi et al 2009, Moazami et al 2012, Quinn et al 2012.During excess light conditions, increased production of damaging reactive oxygen species as byproducts of photosynthesis has been described for algae and plants (Anderson et al 1995). For Nannochloropsis, various aspects of the high light response have been investigated so far, including changes in pigmentation and ultrastructure (Sukenik et al 1989, Fisher et al 1996, Fisher et al 1998, the xanthophyll cycle (Gentile & Blanch 2001), lipid composition (Sukenik et al 1993, non-photochemical quenching system (NPQ; Cao et al 2013) and the photosynthetic response (Fisher et al 1996, Tamburic et al 2014.…”

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confidence: 99%

Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)

Yangüez

Lovazzano

Contreras–Porcia

et al. 2015

Rev. biol. mar. oceanogr.

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ARTICLEResponse to oxidative stress induced by high light and carbon dioxide (CO 2 ) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)Respuesta a estrés oxidativo generado por alta luz y dióxido de carbono (CO 2 ) en el modelo productor de biodiesel Nannochloropsis salina (Ochrophyta, Eustigmatales) Resumen.-Producto del agotamiento de los recursos mundiales de combustibles fósiles, las microalgas han tomado fuerza como alternativa de biocombustible. Buscando hacer sustentable el proceso, en general se propone realizar los cultivos acoplados a fuentes de emisión de CO 2 , logrando con ello mayores rendimientos en biomasa y mitigando la huella de carbono de los procesos de combustión. Nannochloropsis salina es una microalga de la familia Monodopsidaceae de fácil crecimiento y que produce compuestos de valor comercial, tales como pigmentos esenciales, ácidos grasos poliinsaturados y alta cantidad de lípidos. Estudios previos muestran que en presencia de algunas concentraciones de CO 2 (hasta 2%) se produce un aumento de la biomasa y de la producción de ácidos grasos. Sin embargo, estas condiciones traen consigo una acidificación del medio, condición que afecta la eficiencia del proceso de fotosíntesis y promueve la generación de especies reactivas de oxígeno. En este trabajo, se estudió la respuesta antioxidante de cultivos de Nannochloropsis salina suplementados con CO 2 , por medio de 3 metodologías: analizando la actividad enzimática antioxidante (catalasa, ascorbato peroxidasa y peroxirredoxina), cuantificando los compuestos fenólicos, H 2 O 2 y lipoperóxidos (i.e., marcador de daño celular) y evaluando los niveles de expresión génica. Los resultados sugieren que un aumento en la concentración de CO 2 en el cultivo, junto con alta luz, induce una condición de estrés oxidativo en Nannochloropsis salina. Sin embargo, la respuesta celular observada en esta microalga logra atenuar este estrés, sin afectar su rendimiento global. KarenPalabras clave: Nannochloropsis salina, especies reactivas de oxígeno, enzimas antioxidantes, dióxido de carbono, qPCRAbstract.-Due to overconsumption of fossil fuels, microalgae have arrived as an alternative source of biofuel. Looking forward to generate a sustainable process, it is proposed to couple the cultures to CO 2 emission sources, reaching in this way higher biomass performance and helping in the way with the capture of carbon released by the combustion processes. Nannochloropsis salina is a microalgae from the Monodopsidaceae family, which is easy to grow and produces high value compounds like essential pigments, polyunsaturated fatty acids and high amounts of lipids. Previous studies showed that adding CO 2 to cultures (until 2%) generated an increment in biomass and in the production of fatty acids. However, these conditions also induce acidification of the media, a condition that may promote the generation of oxygen reactive species. In this work, the antioxidant performance of N. salina was studied under different culture conditions invo...

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Nannochloropsis production metrics in a scalable outdoor photobioreactor for commercial applications

Cited by 128 publications

References 32 publications

Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall

Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall

Selection of microalgae for biodiesel production in a scalable outdoor photobioreactor in north China

Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)

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