“…However, it is important to note that RT-PCR does have several limitations. 12,13 These constraints include the requirement for well-equipped laboratory facilities and the inherent complexity associated with its preparation and detection methods. To overcome these constraints, alternative nucleic acid amplification methodologies, such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and microarray technologies, can be employed to devise cost-effective, expeditious, and efficient detection approaches.…”