Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy

Soares, Igor Paulino Mendes; Anselmi, Caroline; Kitagawa, Fernanda Ali; Ribeiro, Rafael Antônio de Oliveira; Leite, Maria Luísa; Costa, Carlos Alberto de Souza; Hebling, Josimeri

doi:10.1016/j.dental.2022.03.006

Cited by 17 publications

(10 citation statements)

References 52 publications

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“…Whit this in mind, we investigated cell compatibility using human dental pulp stem cells (SHEDs), knowing their significant potential for osteogenic differentiation [52], key in periodontal bone regeneration. The results demonstrated no differences in cell viability for the four tested membranes over the three time points, mainly due to insufficient calcium released to stimulate cell proliferation [53,54]. The same happened with the alkaline phosphatase data; after seven days, the Ca-TMP group showed higher mineral differentiation activity but not enough to promote a significant difference between them and the osteogenic medium.…”

Section: Discussionmentioning

confidence: 58%

Calcium Trimetaphosphate-Loaded Electrospun Poly(Ester Urea) Nanofibers for Periodontal Tissue Engineering

Toledo

Anselmi

Dal‐Fabbro

et al. 2023

JFB

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The objective of this research was to create and appraise biodegradable polymer-based nanofibers containing distinct concentrations of calcium trimetaphosphate (Ca-TMP) for periodontal tissue engineering. Poly(ester urea) (PEU) (5% w/v) solutions containing Ca-TMP (15%, 30%, 45% w/w) were electrospun into fibrous scaffolds. The fibers were evaluated using SEM, EDS, TGA, FTIR, XRD, and mechanical tests. Degradation rate, swelling ratio, and calcium release were also evaluated. Cell/Ca-TMP and cell/scaffold interaction were assessed using stem cells from human exfoliated deciduous teeth (SHEDs) for cell viability, adhesion, and alkaline phosphatase (ALP) activity. Analysis of variance (ANOVA) and post-hoc tests were used (α = 0.05). The PEU and PEU/Ca-TMP-based membranes presented fiber diameters at 469 nm and 414–672 nm, respectively. Chemical characterization attested to the Ca-TMP incorporation into the fibers. Adding Ca-TMP led to higher degradation stability and lower dimensional variation than the pure PEU fibers; however, similar mechanical characteristics were observed. Minimal calcium was released after 21 days of incubation in a lipase-enriched solution. Ca-TMP extracts enhanced cell viability and ALP activity, although no differences were found between the scaffold groups. Overall, Ca-TMP was effectively incorporated into the PEU fibers without compromising the morphological properties but did not promote significant cell function.

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Section: Discussionmentioning

confidence: 58%

Calcium Trimetaphosphate-Loaded Electrospun Poly(Ester Urea) Nanofibers for Periodontal Tissue Engineering

Toledo

Anselmi

Dal‐Fabbro

et al. 2023

JFB

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“…In the β-CPP/AlgA/5/3 3D Scaffold was observed the highest positive stimulus, consequently caused by the activation of the intracellular signaling pathways. The ions Ca 2+ , in this 3D Scaffold composition, ensured an effective modulation in the cells, cause its differentiation and produce the mineralized matrix 37 . After passing this ideal concentration (observed that for the group 2:1 ratio), an inhibitory stimulus occurred, due to a toxic behavior caused by the excessive [Ca 2+ ], present in the composition of the 3D Scaffold 62 .…”

Section: In Vitro Testsmentioning

confidence: 90%

β-CPP bioceramics in alginate 3D Scaffolds as a new material for mineralized tissue regeneration

Pinto,

Piazza,

Soares

et al. 2024

Preprint

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In the pursuit of tailored properties for applied bioceramics in bone graft applications, a personalized mixture of precursor base materials is crucial. Hydroxyapatite (HA), beta tricalcium phosphate (β-TCP), and versatile biopolymers serve as base materials to produce personalized mixtures, each presenting its own set of advantages and disadvantages. Combining these materials addresses limitations of individual components, but further improvements require exploring alternative base materials with distinct properties. This study introduces beta calcium pyrophosphate (β-CPP) as a valuable addition to the base materials, exhibiting intermediate biodegradation properties. When combined with biopolymer alginate, β-CPP enables the fabrication of personalized porous 3D scaffolds. Despite β-CPP being an unwanted by-product in mineralized tissue regeneration, this research demonstrates its innovative potential in stimulating cell interaction within porous 3D structures. The β-CPP/Alginate 3D Scaffold, with a 5:3 w/w ratio, significantly enhances mineralization activity compared to the control. This novel composite, showcasing interconnected cells throughout the 3D structure, presents a promising avenue for mineralized tissue regeneration. The study underscores the importance of optimizing both biodegradation and mechanical features in new bioceramics, highlighting β-CPP as a noteworthy candidate with potential commercial implications.

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“…Several scholars have investigated DPSCs’ odontoblastic differentiation on PCL or PCL/PLA electrospun scaffolds with the addition of different active substances. Their choices of dopants included nanohydroxyapatite (nHAP), 83–85 zeolite, 86 calcium hydroxide, and fibronectin. 87 On each of these composite scaffolds, DPSCs showed superior proliferation and odontoblastic differentiation behavior than on untreated scaffolds.…”

Section: Esns Application In Caries Lesionsmentioning

confidence: 99%

Electrospun nanofibers applications in caries lesions: prevention, treatment and regeneration

Chen,

Liu,

et al. 2024

J. Mater. Chem. B

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Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy

Cited by 17 publications

References 52 publications

Calcium Trimetaphosphate-Loaded Electrospun Poly(Ester Urea) Nanofibers for Periodontal Tissue Engineering

Calcium Trimetaphosphate-Loaded Electrospun Poly(Ester Urea) Nanofibers for Periodontal Tissue Engineering

β-CPP bioceramics in alginate 3D Scaffolds as a new material for mineralized tissue regeneration

Electrospun nanofibers applications in caries lesions: prevention, treatment and regeneration

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