Nano-sized selenium attenuates the developmental testicular toxicity induced by di-n-butyl phthalate in pre-pubertal male rats

Rashad, Maha M.; Galal, Mona K.; Abou‐El‐Sherbini, Khaled S.; El-Behairy, Adel M; Gouda, Eman M.; moussa, Said.Z.

doi:10.1016/j.biopha.2018.09.006

Cited by 33 publications

(24 citation statements)

References 47 publications

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“…The amplicon size was con rmed by 2% agarose gel electrophoresis stained with SYBR Safe DNA gel stain (Invitrogen). The β-actin gene was furtherly ampli ed in the same reaction to serve as the reference gene (52). Each measurement was repeated three times, and the values were used to calculate the gene/β-actin ratio, with a value of 1.0 used as the control (calibrator) and the normalized expression ratio was calculated (53).…”

Section: Quantitative Real-time Pcr For Aromatase (Cyp19α1) Genementioning

confidence: 99%

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Azouz

Ali

Abd‐Elsalam

et al. 2020

Preprint

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Background: Complementary remedies such as the Chinese herb ‘Sheng Ma’ (Black cohosh; Actaea racemosa ‘AR’) are being sought to overcome the shortcomings of conventional hormonal and surgical therapies developed for treatment of polycystic ovary syndrome (PCOS). However, the AR hepatotoxicity urges a cautionary warning to be labeled on its products as recommended by the United States Pharmacopeia, where four out of seven hepatotoxic cases in Sweden were possibly associated with black cohosh products.Methods: We investigated the effects, safety, and molecular targets of black cohosh ethanolic extract and/or vitamin C on ovarian functionality and oxidative response in hyperandrogenism-induced PCOS rats. A well-established rat model using oral letrozole, daily, for 21 days was employed then rats received the AR extract with and without vitamin C for 28 days. The hormonal evaluation, antioxidant status, histopathological examination, immunohistochemical analysis, cell proliferation, and the expression ratio of the aromatase (Cyp19α1) gene were evaluated. Additionally, a holistic profiling of AR arsenal of secondary metabolites was performed using ultra high-performance liquid chromatography (UHPLC) coupled with quadrupole high resolution time of flight mass spectrometry (QTOF-MS).Results: Beneficial effects were exerted by AR in PCOS rats via the improved antioxidant status, hormonal profile, lipid profile, glucose level, liver functions, and the induced Ki-67 expression in the granulosa, theca cell layers and interstitial stromal cells. Notably, the combination of AR with vitamin C was not only more effective in reversing the dysregulated levels of testosterone, luteinizing hormone, and mRNA level of Cyp19α1 gene in the PCOS rat, but also safer. The combination regulated both ovarian and hepatic MDA and GSH levels with histological improvement observed in the liver and ovaries. In addition, the untargeted metabolomic profiling enabled the identification of 61 metabolites allocated in five major chemical classes.Conclusion: This study demonstrated the benefit of the combination between AR and vitamin C in mitigating the reproductive and metabolic disorders of PCOS with the elimination of AR hepatotoxic risk.

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Section: Quantitative Real-time Pcr For Aromatase (Cyp19α1) Genementioning

confidence: 99%

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Azouz

Ali

Abd‐Elsalam

et al. 2020

Preprint

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“…The glutathione peroxidase enzyme is protected by the meiotic and mitotic divisions and preventing the destructive effects of DNA breakage. It also prevents damage to sperm by protecting the cell membrane and maintaining its fluidity and stability (29,31). These particles not only can maintain serum testosterone levels but also minimize oxidative damage to Leydig cells by enhancing the antioxidant state.…”

Section: Discussionmentioning

confidence: 99%

Application of Selenium Nanoparticles on Sperm Quantity Indicators in Wistar Rat

et al. 2021

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Background: Antioxidant enzymes and selenium in semen plasma have vital effects on reducing lipid peroxidation of spermatozoid membrane in elderly organisms by preventing the destructive effects of reactive oxygen species. Therefore, the use of antioxidants and selenium is essential for normal testicular function and spermatogenesis. Methods: In this experimental study, 88 male Wistar rats aged 2.5 months (young) and 11 Winstar aged months (old) were separated into 4 groups of control, placebo, experimental 1 (0.2 mg/kg BW), and experimental 2 (0.4). Rats encountered weekly surgery after a week of receiving different treatments. Resection of their testes was used for histological studies. Results: The number of spermatocytes, spermatid, and spermatozoa in young and old rats increased during the study period. In young rats, the highest number of these cells remarked in the third and fourth weeks of the experiment using selenium nanoparticles (ranged from 165.3 to 285.3 × 106). For old rates, the highest number of the abovementioned cells observed in the second week of the experiment (ranged from 143.3 to 146.7 × 106). Variables of treatment, week, and age presented significant effects on the number of testicular germ cells. Also, applied treatments had no significant effect on the sperm quality characteristics of rats. The number of Sertoli and Leydig cells did not show a significant difference compared to the control group. The results showed a positive and highly significant correlation between spermatozoid, spermatocytes, and spermatocytes. The findings also indicated the same genetic and environmental effects on the traits. Therefore, any spermatocyte shift will have a direct influence on the spermatozoid. Conclusions: This study demonstrated that using selenium nanoparticles in young and old age groups in rats could improve testicular germ cells, especially in the old group. Therefore, with growing age and decreasing sperm quality, selenium nanoparticles can be used due to their positive effect on sperm parameters and their low health risk.

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“…Furthermore, in model systems based on prevention of oxidative stress and toxicity by various antioxidants nano-Se behaves as a typical Nrf-2 inducer. For example, co-administration of nano-Se high dose along with di- n -butyl phthalate significantly decreased the level of MDA, and also improved GSH concentration and GPx and SOD activities initially compromised by the toxicant treatment (Rashad et al ., 2018). Similarly, in male rats treated with lead acetate, oxidative stress was observed in thyroid tissues and nano-Se supplementation restored antioxidant defence mechanisms (GPx, catalase (CAT), SOD, and GSH) and expression of iodothyronine deiodinase type 1 which were compromised due to the lead-acetate treatment (Atteia et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

Food for thought: nano-selenium in poultry nutrition and health

Surai

Kochish

2020

Anim. Health. Res. Rev.

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In recent years, nanoparticles have become a fashionable subject of research due to their sizes, shapes, and unique intrinsic physicochemical properties. In particular for the last 5 years, nano-Se has received tremendous attention in terms of its production, characteristic, and possible application for poultry/animal science and medical sciences. Indeed, Nano-Se is shown to be a potential source of Se for poultry/animal nutrition. However, there is an urgent need to address the questions related to nano-Se absorption, assimilation, and metabolism. It is not clear at present if major biological effects of nano-Se are due to Se-protein synthesis, direct antioxidant/prooxidant effects, or both. It is necessary to understand how metallic nano-Se can be converted into H2Se and further to SeCys to be incorporated into selenoproteins. The aforementioned issues must be resolved before nano-Se finds its way to animal/poultry production as a feed supplement and clearly this subject warrants further investigation.

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Nano-sized selenium attenuates the developmental testicular toxicity induced by di-n-butyl phthalate in pre-pubertal male rats

Cited by 33 publications

References 47 publications

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: Promising activity without the risk of hepatic adverse effect

Application of Selenium Nanoparticles on Sperm Quantity Indicators in Wistar Rat

Food for thought: nano-selenium in poultry nutrition and health

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