Nanobead-on-string composites for tendon tissue engineering

Rinoldi, Chiara; Kijeńska, Ewa; Chlanda, Adrian; Choińska, Emilia; Khenoussi, Nabyl; Tamayol, Ali; Khademhosseini, Ali; Święszkowski, Wojciech

doi:10.1039/c8tb00246k

Cited by 54 publications

(35 citation statements)

References 56 publications

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“…Tendon is a highly organized connective tissue built primarily of collagens (about 80% of dry mass of tendons) and elastin (1%) embedded in a matrix composed of water and proteoglycans . It forms a specific construct interposed between muscle and bone in order to enable the movement of a joint by transferring the forces created by muscle to the bone.…”

Section: Introductionmentioning

confidence: 99%

“…However, they do not guarantee full recovery and suffer from many disadvantages such as donor site morbidity, low availability, inflammatory response, and infection risk . To overcome the limitations of the existing treatment methods, researchers have introduced innovative tissue engineered scaffolds incorporating cells and growth factors as well as constructs that simulate the fibrous structure of tendon . Numerous strategies exist based on fiber‐forming techniques, such as electrospinning .…”

Section: Introductionmentioning

confidence: 99%

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Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Rinoldi

Costantini

Kijeńska‐Gawrońska

et al. 2019

Adv Healthcare Materials

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108

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Fiber‐based approaches hold great promise for tendon tissue engineering enabling the possibility of manufacturing aligned hydrogel filaments that can guide collagen fiber orientation, thereby providing a biomimetic micro‐environment for cell attachment, orientation, migration, and proliferation. In this study, a 3D system composed of cell‐laden, highly aligned hydrogel yarns is designed and obtained via wet spinning in order to reproduce the morphology and structure of tendon fascicles. A bioink composed of alginate and gelatin methacryloyl (GelMA) is optimized for spinning and loaded with human bone morrow mesenchymal stem cells (hBM‐MSCs). The produced scaffolds are subjected to mechanical stretching to recapitulate the strains occurring in native tendon tissue. Stem cell differentiation is promoted by addition of bone morphogenetic protein 12 (BMP‐12) in the culture medium. The aligned orientation of the fibers combined with mechanical stimulation results in highly preferential longitudinal cell orientation and demonstrates enhanced collagen type I and III expression. Additionally, the combination of biochemical and mechanical stimulations promotes the expression of specific tenogenic markers, signatures of efficient cell differentiation towards tendon. The obtained results suggest that the proposed 3D cell‐laden aligned system can be used for engineering of scaffolds for tendon regeneration.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Rinoldi

Costantini

Kijeńska‐Gawrońska

et al. 2019

Adv Healthcare Materials

Self Cite

108

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“…The possibility of scaling up the production makes this technique attractive for various applications. In addition to biomedicine [17,18], the best known and described areas where electrospun polymer fibres can be applied are filtration, electronics, membranes, optics and sensors [19,20].…”

Section: Introductionmentioning

confidence: 99%

Processing of (Co)Poly(2-oxazoline)s by Electrospinning and Extrusion from Melt and the Postprocessing Properties of the (Co)Polymers

et al. 2020

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Poly(2-oxazoline) (POx) matrices in the form of non-woven fibrous mats and three-dimensional moulds were obtained by electrospinning and fused deposition modelling (FDM), respectively. To obtain these materials, poly(2-isopropyl-2-oxazoline) (PiPrOx) and gradient copolymers of 2-isopropyl- with 2-n-propyl-2-oxazoline (P(iPrOx-nPrOx)), with relatively low molar masses and low dispersity values, were processed. The conditions for the electrospinning of POx were optimised for both water and the organic solvent. Also, the FDM conditions for the fabrication of POx multi-layer moulds of cylindrical or cubical shape were optimised. The properties of the POx after electrospinning and extrusion from melt were determined. The molar mass of all (co)poly(2-oxazoline)s did not change after electrospinning. Also, FDM did not influence the molar masses of the (co)polymers; however, the long processing of the material caused degradation and an increase in molar mass dispersity. The thermal properties changed significantly after processing of POx what was monitored by increase in enthalpy of exo- and endothermic peaks in differential scanning calorimetry (DSC) curve. The influence of the processing conditions on the structure and properties of the final material were evaluated having in a mind their potential application as scaffolds.

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“…These polymeric mixtures have been used as a matrix to form nanocomposites, such is the case of the mixture formed by PA-6 and PCL, to which silica nanoparticles were incorporated. This polymer nanocomposite has been used to form fibrous scaffolds useful in tissue engineering specifically for tendons, where silica nanoparticles improve the biological activity of scaffolds, in addition to modifying topography, wetting capacity, stiffness and degradation rate [33].…”

Section: Biodegradable Polymer Nanocomposites and Their Application Amentioning

confidence: 99%

Nanocomposite and biodegradable polymers applied to technical textiles

Caicedo

Melo-López

Cabello-Alvarado

et al. 2019

DYNA

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Based on the results of research papers reflected in the scientific literature, the main examples, methods and perspectives for the development of technical textiles are considered. The focus of this work is to concentrate the results obtained for different textile applications (technical textiles) through the use of biodegradable polymers modified and improved with nanoparticles. The techniques for obtaining polymeric nanocomposites, finishing processes, type and structure of textiles are specified. In general, key aspects are identified for a better understanding of the technical challenges and physicochemical effects of the fibers.

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Nanobead-on-string composites for tendon tissue engineering

Abstract: The bead-on-string topography of electrospun nanocomposite scaffolds improves fibroblast response in terms of cell spreading and proliferation.

Cited by 54 publications

References 56 publications

Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Processing of (Co)Poly(2-oxazoline)s by Electrospinning and Extrusion from Melt and the Postprocessing Properties of the (Co)Polymers

Nanocomposite and biodegradable polymers applied to technical textiles

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