Nanocellulose-enriched hydrocolloid-based hydrogels designed using a Ca2+ free strategy based on citric acid

Lungu, Adriana; Cernencu, Alexandra I.; Dinescu, Sorina; Balahura, Roxana; Mereuta, Paul; Costache, Marieta; Syverud, Kristin; Stancu, Izabela‐Cristina; Iovu, Horia

doi:10.1016/j.matdes.2020.109200

Cited by 39 publications

(19 citation statements)

References 61 publications

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“…In this study, we evaluated the potential of 5-FU-loaded ALG or PEC hydrogels as anti-tumor bioplatforms for biomedical applications. The biocompatibility of the CNFs was previously assessed and confirmed in a previous study [ 29 ]. The results of quantitative and qualitative viability tests indicated a strong cytotoxic effect of 5-FU on tumor cells, especially in 3D systems in which the concentration of 5 mg/mL 5-FU was added.…”

Section: Discussionmentioning

confidence: 59%

“…High-methoxylated pectin, extracted from apple with an esterification degree of 70–75%, was purchased from Sigma-Aldrich and subjected to chemical modification in order to yield low-methoxylated pectin according to the protocol described in our previous report [ 28 ]. Aqueous CNFs (1.13% w / v ) with a total acidic content of 835 µmol/g were prepared from never-dried, bleached-kraft pulp from softwood (kindly supplied by StoraEnso TM (Stockholm, Sweden) according to our previous report [ 29 ], using the TEMPO/NaBr/NaClO at pH 10.5, also in agreement with a previous study with NaClO of 2.5 mmol g −1 cellulose [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

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Cellulose Nanofiber-Based Hydrogels Embedding 5-FU Promote Pyroptosis Activation in Breast Cancer Cells and Support Human Adipose-Derived Stem Cell Proliferation, Opening New Perspectives for Breast Tissue Engineering

et al. 2021

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The structure and biocompatibility analysis of a hydrogel based on cellulose nanofibers (CNFs) combined with alginate/pectin (A.CNF or P.CNF) and enriched with 1% or 5% 5-FU revealed more favorable properties for the cellular component when pectin was dispersed within CNFs. 5-Fluorouracil (5-FU) is an antimetabolite fluoropyrimidine used as antineoplastic drug for the treatment of multiple solid tumors. 5-FU activity leads to caspase-1 activation, secretion and maturation of interleukins (IL)-1, IL-18 and reactive oxygen species (ROS) generation. Furthermore, the effects of embedding 5-FU in P.CNF were explored in order to suppress breast tumor cell growth and induce inflammasome complex activation together with extra- and intracellular ROS generation. Exposure of tumor cells to P.CNF/5-FU resulted in a strong cytotoxic effect, an increased level of caspase-1 released in the culture media and ROS production—the latter directly proportional to the concentration of anti-tumor agent embedded in the scaffolds. Simultaneously, 5-FU determined the increase of p53 and caspase-1 expressions, both at gene and protein levels. In conclusion, P.CNF/5-FU scaffolds proved to be efficient against breast tumor cells growth due to pyroptosis induction. Furthermore, biocompatibility and the potential to support human adipose-derived stem cell growth were demonstrated, suggesting that these 3D systems could be used in soft tissue reconstruction post-mastectomy.

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Section: Discussionmentioning

confidence: 59%

Section: Methodsmentioning

confidence: 99%

Cellulose Nanofiber-Based Hydrogels Embedding 5-FU Promote Pyroptosis Activation in Breast Cancer Cells and Support Human Adipose-Derived Stem Cell Proliferation, Opening New Perspectives for Breast Tissue Engineering

et al. 2021

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“…In addition, the surface properties of individual cellulose nanofibrils may be controlled through oxidative treatments. These advantages have made CNFs a preferred nano‐filler for many studies involving the development of materials for TE applications (Aarstad et al, 2017; Campodoni et al, 2019; Carlström et al, 2020; Cheng et al, 2019; Doench et al, 2018, 2019; Du et al, 2019; Gorgieva et al, 2017; Hickey & Pelling, 2019; Lohrasbi et al, 2020; Lungu et al, 2021; Maharjan et al, 2021; Mirtaghavi et al, 2020; Syverud, 2017; Tavakoli et al, 2021; Y. Zhang et al, 2019).…”

Section: Nanocomposite Hydrogels Incorporating Functionalized Nanomaterialsmentioning

confidence: 99%

Nanoengineered biomimetic hydrogels: A major advancement to fabricate 3D‐printed constructs for regenerative medicine

Cernencu

Dinu

Stancu

et al. 2022

Biotech & Bioengineering

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Nanostructured compounds already validated as performant reinforcements for biomedical applications together with different fabrication strategies have been often used to channel the biophysical and biochemical features of hydrogel networks. Ergo, a wide array of nanostructured compounds has been employed as additive materials integrated with hydrophilic networks based on naturally‐derived polymers to produce promising scaffolding materials for specific fields of regenerative medicine. To date, nanoengineered hydrogels are extensively explored in (bio)printing formulations, representing the most advanced designs of hydrogel (bio)inks able to fabricate structures with improved mechanical properties and high print fidelity along with a cell‐interactive environment. The development of printing inks comprising organic–inorganic hybrid nanocomposites is in full ascent as the impact of a small amount of nanoscale additive does not translate only in improved physicochemical and biomechanical properties of bioink. The biopolymeric nanocomposites may even exhibit additional particular properties engendered by nano‐scale reinforcement such as electrical conductivity, magnetic responsiveness, antibacterial or antioxidation properties. The present review focus on hydrogels nanoengineered for 3D printing of biomimetic constructs, with particular emphasis on the impact of the spatial distribution of reinforcing agents (0D, 1D, 2D). Here, a systematic analysis of the naturally‐derived nanostructured inks is presented highlighting the relationship between relevant length scales and size effects that influence the final properties of the hydrogels designed for regenerative medicine.

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“…The swelling ratio of freeze-dried CNFs-based hydrogels was determined by using a gravimetric method [17]. Initially, freeze-dried CNFs-based hydrogels were cut into 2 cm × 2 cm pieces, and their dry weights (W d ) were measured.…”

Section: Swelling Ratiomentioning

confidence: 99%

“…CNFs appeared to be well-integrated with LMP and SA since no separation phase was noticed. The addition of crosslinking agents such as Ca, Ca + Ci, or Ci solutions was an important parameter that strongly influenced the morphology and porosity [17].…”

Section: Preparation and Morphological Characteristics Of Cnfs-based ...mentioning

confidence: 99%

Composite Nanocellulose Fibers-Based Hydrogels Loading Clindamycin HCl with Ca2+ and Citric Acid as Crosslinking Agents for Pharmaceutical Applications

et al. 2021

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Biocomposite hydrogels based on nanocellulose fibers (CNFs), low methoxy pectin (LMP), and sodium alginate (SA) were fabricated via the chemical crosslinking technique. The selected CNFs-based hydrogels were loaded with clindamycin hydrochloride (CM), an effective antibiotic as a model drug. The properties of the selected CNFs-based hydrogels loaded CM were characterized. The results showed that CNFs-based hydrogels composed of CNFs/LMP/SA at 1:1:1 and 2:0.5:0.5 mass ratios exhibited high drug content, suitable gel content, and high maximum swelling degree. In vitro assessment of cell viability revealed that the CM-incorporated composite CNFs-based hydrogels using calcium ion and citric acid as crosslinking agents exhibited high cytocompatibility with human keratinocytes cells. In vitro drug release experiment showed the prolonged release of CM and the hydrogel which has a greater CNFs portion (C2P0.5A0.5/Ca + Ci/CM) demonstrated lower drug release than the hydrogel having a lesser CNFs portion (C1P1A1/Ca + Ci/CM). The proportion of hydrophilic materials which were low methoxy pectin and sodium alginate in the matrix system influences drug release. In conclusion, biocomposite CNFs-based hydrogels composed of CNFs/LMP/SA at 1:1:1 and 2:0.5:0.5 mass ratios, loading CM with calcium ion and citric acid as crosslinking agents were successfully developed for the first time, suggesting their potential for pharmaceutical applications, such as a drug delivery system for healing infected wounds.

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Nanocellulose-enriched hydrocolloid-based hydrogels designed using a Ca2+ free strategy based on citric acid

Cited by 39 publications

References 61 publications

Cellulose Nanofiber-Based Hydrogels Embedding 5-FU Promote Pyroptosis Activation in Breast Cancer Cells and Support Human Adipose-Derived Stem Cell Proliferation, Opening New Perspectives for Breast Tissue Engineering

Cellulose Nanofiber-Based Hydrogels Embedding 5-FU Promote Pyroptosis Activation in Breast Cancer Cells and Support Human Adipose-Derived Stem Cell Proliferation, Opening New Perspectives for Breast Tissue Engineering

Nanoengineered biomimetic hydrogels: A major advancement to fabricate 3D‐printed constructs for regenerative medicine

Composite Nanocellulose Fibers-Based Hydrogels Loading Clindamycin HCl with Ca2+ and Citric Acid as Crosslinking Agents for Pharmaceutical Applications

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