Nanoclay-based sensor composites for the facile detection of molecular antioxidants

Szerlauth, Adél; Szalma, Lilla; Muráth, Szabolcs; Sáringer, Szilárd; Varga, Gábor; Li, Li; Szilágyi, István

doi:10.1039/d1an02352g

Cited by 9 publications

(5 citation statements)

References 56 publications

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“…The physical barrier created by lignin deposition is more effective at preventing viral infection [ 72 ]. Our results match those of other studies [ 52 , 73 ] that considered the ability of different nanoparticles to increase the activity of plant enzymes in response to ROS. Additionally, ROS can harm the plant’s membrane lipids, proteins, and DNA, leading to symptom development rather than defense or acclimation if the timing and magnitude of ROS accumulation are not tightly controlled by the plant’s antioxidant system [ 74 , 75 ].…”

Section: Resultssupporting

confidence: 91%

Foliar Application of Nanoclay Promotes Potato (Solanum tuberosum L.) Growth and Induces Systemic Resistance against Potato Virus Y

Aseel

Abdelkhalek

Al-Otibi

et al. 2022

Viruses

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Potato virus Y (PVY) is one of the most harmful phytopathogens. It causes big problems for potatoes and other important crops around the world. Nanoclays have been extensively studied for various biomedical applications. However, reports on their interactions with phytopathogens, particularly viral infections, are still limited. In this study, the protective activity of Egyptian nanoclay (CE) and standard nanoclay (CS) against PVY was evaluated on potato (Solanum tuberosum L.) plants. Their physicochemical and morphological properties were examined with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and energy dispersive spectrometer (EDS). SEM and TEM analyses revealed that CE has a spherical and hexagonal structure ranging from 20 to 80 nm in size, while CS has boulder-like and tubular structures of about 320 nm in size. FTIR and EDS showed that both nanoclay types have different functional groups and contain many vital plant nutrients that are necessary for every stage and process of the plant, including development, productivity, and metabolism. Under greenhouse conditions, a 1% nanoclay foliar application enhanced potato growth, reduced disease symptoms, and reduced PVY accumulation levels compared with non-treated plants. Significant increases in levels of antioxidant enzymes (PPO and POX) and considerable decreases in oxidative stress markers (MDA and H2O2) were also reported. Moreover, a significant increase in the transcriptional levels of defense-related genes (PAL-1, PR-5, and CHI-2) was observed. All experiment and analysis results indicate that the CE type is more effective than the CS type against PVY infection. Based on these results, the foliar applications of nanoclay could be used to manage plant viral infections in a way that is both effective and environmentally friendly. To our knowledge, this is the first report of the antiviral activity of the foliar application of nanoclay against PVY infection.

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Section: Resultssupporting

confidence: 91%

Foliar Application of Nanoclay Promotes Potato (Solanum tuberosum L.) Growth and Induces Systemic Resistance against Potato Virus Y

Aseel

Abdelkhalek

Al-Otibi

et al. 2022

Viruses

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“…Chemical sensors are simple, inexpensive, stable, highly sensitive, and specific to the particular analyte. For online and real-time analysis, electrochemical sensors can be used [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Efficient and Non-Enzymatic Electrochemical Sensors for the Detection of Sucrose

Asghar

Mustafa

Jabeen

et al. 2023

Sensors

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Molecularly imprinted polymers have been used for the creation of an electrochemical sensor for the detection of sucrose, which are modified by using functionalized graphene (fG). Using AIBN as the free radical initiator and sucrose as the template, imprinted polymers are synthesized. The monomer, 4,4′-diisocyanatodiphenylmethane (DPDI), has both proton donor groups (N-H or O-H) and lone-pair donor groups (C=O). By creating H-bonds with electron donor groups (C=O), the proton donor group in this polymer may interact with the sugar molecule serving as its template. The sensor signals have improved as a result of the interaction between the monomer and the template. Thermogravimetric and differential thermal analysis (TGA/DTA) curves, scanning electron microscopy (SEM), and FT-IR spectroscopy have been employed to characterize the fabricated receptors. The fabricated sensor has exhibited a limit of detection of 16 ppb for the target analyte that is highly sensitive, linear, reversible, regenerative, and selective. Moreover, the sensor’s stability, reproducibility, and reusability have been evaluated for six months, following the device’s manufacturing, and the results revealed similar responses with the percentage error of less than 1%. Most importantly, this sensor has demonstrated a quick response time, which is very sensitive, stable, and selective.

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“…A detailed comparison of different methods for detecting Cu 2+ . References [32][33][34][35][36][37][38][39][40][41] are cited in the supplementary materials.…”

Section: Supplementary Materialsmentioning

confidence: 99%

A Cascade Signal Amplification Strategy for the Ultrasensitive Fluorescence Detection of Cu2+ via λ-Exonuclease-Assisted Target Recycling with Mismatched Catalytic Hairpin Assembly

Liu,

et al. 2023

Biosensors

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Herein, an ultrasensitive DNAzyme-based fluorescence biosensor for detecting Cu2+ was designed using the cascade signal amplification strategy, coupling λ-exonuclease-assisted target recycling and mismatched catalytic hairpin assembly (MCHA). In the designed detection system, the target, Cu2+, can activate the Cu2+-dependent DNAzyme to cause a cleavage reaction, releasing ssDNA (tDNA). Then, tDNA binds to hairpin DNA (H0) with an overhanging 5′-phosphorylated terminus to form dsDNA with a blunt 5′-phosphorylated terminus, which activates the dsDNA to be digested by λ-Exo and releases tDNA along with another ssDNA (iDNA). Subsequently, the iDNA initiates MCHA, which can restore the fluorescence of carboxyfluorescein (FAM) previously quenched by tetramethylrhodamine (TAMRA), resulting in a strong fluorescent signal. Furthermore, MCHA efficiently improves the signal-to-noise ratio of the detection system. More importantly, tDNA recycling can be achieved with the λ-Exo digestion reaction to release more iDNA, efficiently amplifying the fluorescent signal and further improving the sensitivity to Cu2+ with a detection limit of 60 fM. The practical application of the developed biosensor was also demonstrated by detecting Cu2+ in real samples, proving it to be an excellent analytical strategy for the ultrasensitive quantification of heavy metal ions in environmental water sources.

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Nanoclay-based sensor composites for the facile detection of molecular antioxidants

Abstract: The detection and quantification of antioxidant molecules is an important task in food science, fine chemical industry and healthcare. Antioxidants help preventing the deterioration of nutrition and healthcare products, while...

Cited by 9 publications

References 56 publications

Foliar Application of Nanoclay Promotes Potato (Solanum tuberosum L.) Growth and Induces Systemic Resistance against Potato Virus Y

Foliar Application of Nanoclay Promotes Potato (Solanum tuberosum L.) Growth and Induces Systemic Resistance against Potato Virus Y

Fabrication of Efficient and Non-Enzymatic Electrochemical Sensors for the Detection of Sucrose

A Cascade Signal Amplification Strategy for the Ultrasensitive Fluorescence Detection of Cu2+ via λ-Exonuclease-Assisted Target Recycling with Mismatched Catalytic Hairpin Assembly

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