Tuberculosis (TB) is an infectious and contagious disease caused by various strains of Mycobacterium and the foremost leading cause of death of animal and human population worldwide. Currently available conventional and modern diagnostic techniques are useful but they are neither rapid nor cost effective for early and accurate diagnosis, represents a cornerstone to eradicate TB worldwide by 2030. To overcome this awful situation an innovative strategy i.e. nanotechnology is helpful for early diagnosis of tuberculosis in short time with increased sensitivity and specificity through nano sized biomolecular interaction viz. enhanced visualization of fluorescent signals, visual colorimetric signals of amplified DNA product, nanofluidic technology, nuclear magnetic resonance technology (NMR) and prototype miniaturized device etc. Nanotechnology has introduced new paradigms for molecular diagnostics. However, some constraints of nanodiagnostic tool still there that make obstacle in its worldwide diagnostic use for TB.