Nanodiamond for biolabelling and toxicity evaluation in the zebrafish embryo in vivo

Abstract

Nanodiamond (ND) has been proposed for various biomedical applications, including bioimaging, biosensing and drug delivery, owing to its physical-chemical properties and biocompatibility. Particularly, ND has been demonstrated as fluorescence- and Raman-detectable labels in many cellular models. Different surface functionalization methods have been developed, varying the ND's surface properties and rendering the possibility to attach biomolecules to provide interaction with biological targets. For this, toxicity is of major concern in animal models. Aside from cellular models, a cost-effective animal test will greatly facilitate the development of applications. In this study, we use the rapid, sensitive and reproducible zebrafish embryo model for in vivo nanotoxicity test. We optimize the conditions for using this animal model and analyze the zebrafish embryonic development in the presence of ND. ND is observed in the embryo in vivo using laser confocal fluorescence microscopy and fluorescence lifetime imaging. Using the zebrafish model for a safety evaluation of ND-based nanolabel is discussed.

Haemocompatibility of Modified Nanodiamonds

“…With the increasing push towards the development of novel and more advanced carbon-based biosensing or medicinal products, the issue of biocompatibility has been receiving more attention [15,16]. Many research groups have been dealing with biocompatibility issues involving different biological objects, such as cells, blood, and microorganisms [17,18,19,20]. While the majority of studies present a positive opinion on nanodiamond compatibility in general [21,22,23], this is not always the case.…”

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

“…In addition, adsorption of macromolecules (such as berberine in this case) can slightly change the photoluminescence of the observed spectrum (Fig. 4), but does not affect the imaging [44,45]. So, wide application of berberine in biophotonics can be expected due to its bright fluorescence, even in polar solvent like PBS, which can be used to observe the delivery into cell and localization of the drug inside in comparison with ND-drug complex.…”

The conformation of bovine serum albumin adsorbed to the surface of single all‐dielectric nanoparticles following light‐induced heating

“…Basically, the globular protein BSA is commonly used to form a protein corona on plasmonic NPs as well as to bind to the surface of non‐metallic NPs . To coat our NPs, an aqueous solution of BSA (0.1 g/mL) was dropped (0.05 mL) onto these particles on the gold film, which was rotating at 100 Hz at room temperature in air.…”