Nanoengineered Sprayable Therapy for Treating Myocardial Infarction

Abstract

We report the development, as well as the in vitro and in vivo testing, of a sprayable nanotherapeutic that uses surface engineered custom-designed multiarmed peptide grafted nanogold for on-the-spot coating of an infarcted myocardial surface. When applied to mouse hearts, 1 week after infarction, the spray-on treatment resulted in an increase in cardiac function (2.4-fold), muscle contractility, and myocardial electrical conductivity. The applied nanogold remained at the treatment site 28 days postapplication with no off-target organ infiltration. Further, the infarct size in the mice that received treatment was found to be <10% of the total left ventricle area, while the number of blood vessels, prohealing macrophages, and cardiomyocytes increased to levels comparable to that of a healthy animal. Our cumulative data suggest that the therapeutic action of our spray-on nanotherapeutic is highly effective, and in practice, its application is simpler than other regenerative approaches for treating an infarcted heart.

Degradable carrier-free spray hydrogel based on self-assembly of natural small molecule for prevention of postoperative adhesion

“…The administration of the spray solves the problem of non-contact drug delivery, which greatly reduces the possibility of secondary contamination during administration and also accomplishes the customization and convenience of administration [ [60] , [61] , [62] ]. Spray applications also offer high-throughput treatments deposition straight to the uneven surfaces of the whole target location for later sustained release [ 63 , 64 ].…”

Nanotechnology translation in vascular diseases: From design to the bench

“…Exosomes derived from mesenchymal stem cells (MSCs) are the other important component of paracrine factors in cardiac regenerative stem cells. A minimally invasive exocrine spray based on MSC exosomes was also reported to improve cardiac function, reduce fibrosis, and promote endogenous angiogenesis of the injured heart (Muñoz et al, 2022; Yao et al, 2021).…”

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

“…103 Unlike traditional hydrogels, a nanoengineered sprayable therapy used nanoengineered peptide-based materials as a spray-on treatment in a clinically relevant murine MI model with initiated scar formation (humans: 4−14 days, mice: 2−7 days). 104 3.6. Gelation Time.…”

“…Although cell permeable peptide V1-Cal has a limitation of a short half-life, coassembly of V1-Cal with hydrogelator Nap-Phe-Phe-Tyr (NapFFY) achieved a sustained release of V1-Cal beyond 14 days, eventually reducing release of inflammatory factors and downregulating expression and activation of TRPV1 . Unlike traditional hydrogels, a nanoengineered sprayable therapy used nanoengineered peptide-based materials as a spray-on treatment in a clinically relevant murine MI model with initiated scar formation (humans: 4–14 days, mice: 2–7 days) …”