Nanofiber could deliver lactic acid bacteria to the intestine of ruminant in vitro experiment

Chang, Xiaofeng; Dasen, Liu; Lambo, Modinat Tolani

doi:10.1111/jpn.13660

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…Storage temperature plays a crucial role in affecting the viability of probiotic microcapsules. Low‐temperature storage has been widely used for this purpose (Chang et al, 2023). Results from Figure 4C demonstrate a difference in viability between free bacteria and coated bacteria after 42 days of storage.…”

Section: Resultsmentioning

confidence: 99%

Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Wu,

Chang,

Zhang

et al. 2024

Food Bioengineering

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To address the issues of probiotic activity loss during storage and feeding, as well as the limited efficacy of single probiotics, a solution was devised by embedding a mixture of Bacillus coagulans SN‐8 (SN‐8) and Saccharomyces boulardii SN‐6 (SN‐6) in a gel. The initial step involved screening the probiotic microcapsules' preparation method and wall material. Using sodium alginate and β‐cyclodextrin as composite wall material and chitosan as the outer coating material allowed for an embedding rate of 82.11% in composite probiotic microcapsules prepared by the air atomization method. Next, in vitro, simulated digestion experiments were conducted to determine the number of viable bacteria and the release rate of the microcapsules. The results showed that compared to the free strain, the mixed probiotic microcapsules retained a survival rate of 67.5% after 3 h of simulated gastric juice exposure and 70.56% after 42 days of storage at 4°C. This demonstrated higher survival rates and storage stability. The prepared probiotic microcapsules were then administered to dairy cows. 16S rDNA gene sequencing showed that consumption of the microcapsules reduced the number of harmful bacteria, such as Paeniclostridium, in the intestinal tract of dairy cows while accelerating the growth of beneficial bacteria, such as Bifidobacterium. In particular, this resulted in a significant improvement in the lactation performance of the cows, with a 4.5% increase in milk fat content, a 92.5% increase in milk protein content, and a 3.5% increase in milk urea nitrogen content (6.75 mL/dL). In conclusion, probiotic microcapsules can effectively regulate intestinal flora, improving milk production, and quality in dairy cows.

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Section: Resultsmentioning

confidence: 99%

Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Wu,

Chang,

Zhang

et al. 2024

Food Bioengineering

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Advances of Rumen Functional Bacteria and the Application of Micro-Encapsulation Fermentation Technology in Ruminants: A Review

et al. 2022

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Rumen functional bacteria are crucial for the homeostasis of rumen fermentation and micro-ecology. Cellulolytic bacteria, amylolytic bacteria, protein- and fat-degrading bacteria, lactic acid-producing bacteria, lactic acid-consuming bacteria, methanogens, and others can all be found in the rumen flora and help the host and other microorganisms convert feed into energy. For instance, Ruminococcus flavefaciens, Ruminococcus albus, and Fibrobacter succinogenes are the three most prevalent fiber-degrading bacteria. The digestion and metabolism of various nutrients and the absorption in rumen epithelium can greatly enhance host defense mechanisms and health production in ruminants. However, directly feeding live bacteria is prone to negative environmental effects. Therefore, the micro-encapsulation of film-forming and acid-resistant wall materials can become a great means of encapsulating naked bacteria into tiny particles. It can maintain the activity of functional flora, boost the function of the intestinal barrier, and improve its capacity for colonization on the surface of the rumen and colon mucosa. Therefore, the present review evaluates the latent progress of main functional bacteria and the applied techniques of micro-encapsulation in the rumen, in order to provide more references for the development and application of rumen-functional bacteria.

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Nanofiber could deliver lactic acid bacteria to the intestine of ruminant in vitro experiment

Cited by 2 publications

References 41 publications

Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Advances of Rumen Functional Bacteria and the Application of Micro-Encapsulation Fermentation Technology in Ruminants: A Review

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