Nanomaterial designing strategies related to cell lysosome and their biomedical applications: A review

Rathore, Bhowmira; Sunwoo, Kyoung; Jangili, Paramesh; Kim, Jiseon; Kim, Ji Hyeon; Huang, Meina; Xiong, Jie; Sharma, Amit; Yang, Zhigang; Qu, Junle; Kim, Jong Seung

doi:10.1016/j.biomaterials.2019.05.002

Cited by 105 publications

(74 citation statements)

References 93 publications

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“…Thus, these data confirm only mild lysosomal impairment induced by NPs. The end point of NP trafficking by the cell is a formation of mature endolysosomes [63][64][65], which are characterized by the presence of Rab7/LAMP1-vesicles [74,75]. Indeed, NPs treatment lead to significant increase in Rab7/LAMP1 colocalization in all three cell lines (Figure 6a and Supplementary Figure S9), indicating that NP treatment leads to the formation of terminal endocytic vesicles.…”

Section: Nanoparticles Alter Lysosomal Functionmentioning

confidence: 90%

Section: Nanoparticles Alter Lysosomal Functionmentioning

confidence: 99%

“…It is widely accepted that cells engulf nanomaterials by endocytosis process and finally traffic them to the lysosomes [63][64][65]. Thus, it is not a surprise that lysosomes play a crucial role in determining the fate of the nanomaterial [63][64][65]. Overall, lysosomes have been found to actively participate and regulate important parts of cellular metabolism, such as nutrient sensing, transcriptional regulation, and metabolic homeostasis [27,66,67].…”

Section: Nanoparticles Alter Lysosomal Functionmentioning

confidence: 99%

“…Emerging evidence suggest that various types of nanoparticles, including iron oxide, modulate autophagy in a lysosome-dependent manner [28,63,71,[76][77][78]. Indeed, it is widely accepted that NPs may induce autophagy dysfunction via either overstimulation of autophagic flux or inhibition of autophagosome degradation [28,63,71,[76][77][78]. In fact, the detailed mechanism of how iron oxide NPs affect autophagy function is not fully understood.…”

Section: Iron Oxide Nanoparticles Modulate Autophagic Flux and Mtor Amentioning

confidence: 99%

“…The fact, that NP-cell interactions vary greatly in distinct cell types, is widely accepted [16,[63][64][65]90,91]. However, studies that directly compare NP-mediated effects in different cell lines of the same lineage are still rather limited.…”

Section: P53 Sub-cellular Localization Mediates Hepatic Cell Responsementioning

confidence: 99%

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Iron Oxide Nanoparticle-Induced Autophagic Flux Is Regulated by Interplay between p53-mTOR Axis and Bcl-2 Signaling in Hepatic Cells

Uzhytchak

Smolková

Lunová

et al. 2020

Cells

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Iron oxide-based nanoparticles have been repeatedly shown to affect lysosomal-mediated signaling. Recently, nanoparticles have demonstrated an ability to modulate autophagic flux via lysosome-dependent signaling. However, the precise underlying mechanisms of such modulation as well as the impact of cellular genetic background remain enigmatic. In this study, we investigated how lysosomal-mediated signaling is affected by iron oxide nanoparticle uptake in three distinct hepatic cell lines. We found that nanoparticle-induced lysosomal dysfunction alters sub-cellular localization of pmTOR and p53 proteins. Our data indicate that alterations in the sub-cellular localization of p53 protein induced by nanoparticle greatly affect the autophagic flux. We found that cells with high levels of Bcl-2 are insensitive to autophagy initiated by nanoparticles. Altogether, our data identify lysosomes as a central hub that control nanoparticle-mediated responses in hepatic cells. Our results provide an important fundamental background for the future development of targeted nanoparticle-based therapies.

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Section: Nanoparticles Alter Lysosomal Functionmentioning

confidence: 90%

Section: Nanoparticles Alter Lysosomal Functionmentioning

confidence: 99%

Section: Nanoparticles Alter Lysosomal Functionmentioning

confidence: 99%

Section: Iron Oxide Nanoparticles Modulate Autophagic Flux and Mtor Amentioning

confidence: 99%

Section: P53 Sub-cellular Localization Mediates Hepatic Cell Responsementioning

confidence: 99%

See 3 more Smart Citations

Iron Oxide Nanoparticle-Induced Autophagic Flux Is Regulated by Interplay between p53-mTOR Axis and Bcl-2 Signaling in Hepatic Cells

Uzhytchak

Smolková

Lunová

et al. 2020

Cells

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Revealing the Phagosomal pH Regulation and Inflammation of Macrophages after Endocytosing Polyurethane Nanoparticles by A Ratiometric pH Nanosensor

et al. 2020

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on human safety. [2] Macrophages are specialized cells in the first line of defense in determining essential aspects of host exposure to NPs and initiating clearance through regulating innate immunity. [3] In general, naive macrophages (M0 phenotype) can be polarized into M1 (classically activated phenotype) or M2 (alternatively activated phenotype) in response to the microenvironment of stimuli. The size, form, surface hydrophobicity, and charge of NPs may influence the recognition and uptake of NPs by immune cells. [4,5] For instance, macrophages can be activated by exposure to NPs, such as silicon dioxide and titanium dioxide, [6-8] but remained silent when these NPs were encapsulated in the PEGylated polymer. [9] Even though the interaction between NPs and immune system has been investigated for targeted immunotherapy, [7,8] little is known about the role of NPs, including the chemical structures and surface properties, in the autophagy and relevant functions of macrophages. Synthetic polymers such as polylactic acid (PLA), polyglycolic acid, poly(ε-caprolactone) (PCL), and poly(lactic-co-glycolic acid) (PLGA) have been widely used in the biological applications for many years. In recent years, biodegradable polyurethane (PU) has attracted attention in biomedical fields because of their excellent elasticity, biocompatibility, biodegradability, The effect of the intracellular pH of macrophages after taking up biodegradable polymer nanoparticles (NPs) on immunomodulating functions has not been explored so far. Previous studies have demonstrated that biodegradable polyurethane (PU) NPs exhibit immunosuppressive activity. Yet, the intracellular mechanism is not clearly understood. In this study, a uniquely designed pH nanosensor is employed for tracking the intracellular pH value of macro phages to reveal the intracellular journey of PU NPs and to clarify the intracellular pH effect on the corresponding inflammatory response. First, fluorescent mesoporous silica nanoparticles (FRMSNs) is used to detect the pH change in macrophages after endo/phagocytosis of PU NPs. Second, PU is coated on the external surface of FRMSNs to examine the intracellular trafficking process of PU in the macrophages. The results show that the majority of PU-coated FRMSNs remain to stay at the cytosol-early endosome/ phagosome regions. The intracellular pH value and other supporting results show that the immune response of PU NPs may be correlated to their internalization journey. The retardation in the degradation process of the PU NPs may intervene with the lysosome activity and repress the immunostimulatory effect, which contributes to the low immune response of PU NPs.

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Near‐Infrared‐Enpowered Nanomotor‐Mediated Targeted Chemotherapy and Mitochondrial Phototherapy to Boost Systematic Antitumor Immunity

Xiao

2022

Adv Healthcare Materials

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Phototherapy is an important strategy to inhibit tumor growth and activate antitumor immunity. However, the effect of photothermal/photodynamic therapy (PTT/PDT) is restricted by limited tumor penetration depth and unsatisfactory potentiation of antitumor immunity. Here, a near‐infrared (NIR)‐driven nanomotor is constructed with a mesoporous silicon nanoparticle (MSN) as the core, end‐capped with Antheraea pernyi silk fibroin (ApSF) comprising arginine–glycine–aspartate (RGD) tripeptides. Upon NIR irradiation, the resulting ApSF‐coated MSNs (DIMs) loading with photosensitizers (ICG derivatives, IDs) and chemotherapeutic drugs (doxorubicin, Dox) can efficiently penetrate into the internal tumor tissues and achieve effective phototherapy. Combined with chemotherapy, a triple‐modal treatment (PTT, PDT, and chemotherapy) approach is developed to induce the immunogenic cell death of tumor cells and to accelerate the release of damage‐associated molecular patterns. In vivo results suggest that DIMs can promote the maturation of dendritic cells and surge the number of infiltrated immune cells. Meanwhile, DIMs can polarize macrophages from M2 to M1 phenotypes and reduce the percentages of immunosuppressive Tregs, which reverse the immunosuppressive tumor microenvironment and activate systemic antitumor immunity. By achieving synergistic effects on the tumor inhibition and the antitumor immunity activation, DIMs show great promise as new nanoplatforms to treat metastatic breast cancer.

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Nanomaterial designing strategies related to cell lysosome and their biomedical applications: A review

Cited by 105 publications

References 93 publications

Iron Oxide Nanoparticle-Induced Autophagic Flux Is Regulated by Interplay between p53-mTOR Axis and Bcl-2 Signaling in Hepatic Cells

Iron Oxide Nanoparticle-Induced Autophagic Flux Is Regulated by Interplay between p53-mTOR Axis and Bcl-2 Signaling in Hepatic Cells

Revealing the Phagosomal pH Regulation and Inflammation of Macrophages after Endocytosing Polyurethane Nanoparticles by A Ratiometric pH Nanosensor

Near‐Infrared‐Enpowered Nanomotor‐Mediated Targeted Chemotherapy and Mitochondrial Phototherapy to Boost Systematic Antitumor Immunity

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