Nanomaterials towards fabrication of cholesterol biosensors: Key roles and design approaches

Saxena, Urmila; Das, Asim Bikas

doi:10.1016/j.bios.2015.08.042

Cited by 105 publications

(48 citation statements)

References 131 publications

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“…Unfortunately, biocompatibility-based issues cannot be easily circumvented and adequate pre- and post-calibration procedures are needed in order to properly evaluate in vivo AEBs measurements (Wilson and Gifford, 2004; Wahono et al, 2012). However it is expected that the forthcoming generation of biosensors, either based on nanoscale or polymeric materials, will greatly help reducing biocompatibility issues (Nichols et al, 2013; Weltin et al, 2014; Saxena and Das, 2016). …”

Section: In Vivo Etoh and Acd Detection: Invasive Approachesmentioning

confidence: 99%

On the Accuracy of In Vivo Ethanol and Acetaldehyde Monitoring, a Key Tile in the Puzzle of Acetaldehyde as a Neuroactive Agent

Enrico

Diana

2017

Front. Behav. Neurosci.

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Over the last 20 years researchers have explored the postulated role of acetaldehyde (ACD) as a mediator of some of the actions of ethanol (EtOH) in the central nervous system (CNS). However, efforts have been hampered mainly by the difficulty of directly measuring in vivo EtOH and ACD levels in the CNS and thus, our knowledge is based on indirect evidences. Although technically challenging, the development of reliable methods for in vivo measurement of ACD and EtOH is of paramount importance to solve the “puzzle of acetaldehyde as a neuroactive agent.” In this short review we discuss the recent advances on brain EtOH pharmacokinetic and state-of-the-art available techniques that could be used for in vivo detect EtOH and ACD both non-invasively (magnetic resonance spectroscopy), and invasively (microdialysis and biosensors). Among the different in vivo sampling techniques described, particular emphasis is paid to the field of enzyme-based amperometric biosensors. Biosensors have gained much attention in recent years for their ability to online monitor biological signals in vivo, and several micro- and nano-structured devices have been successfully used for in vivo studies. Owing to their high temporal and spatial resolution, biosensors could provide the adequate technology for studying in vivo EtOH pharmacokinetic.

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Section: In Vivo Etoh and Acd Detection: Invasive Approachesmentioning

confidence: 99%

On the Accuracy of In Vivo Ethanol and Acetaldehyde Monitoring, a Key Tile in the Puzzle of Acetaldehyde as a Neuroactive Agent

Enrico

Diana

2017

Front. Behav. Neurosci.

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“…It acts as an essential raw material in the synthesis of adrenal cortex hormones and sex hormones and plays an important role in strengthening and maintaining of blood vessels. [1][2][3][4] A large body of medical data indicates that high cholesterol (based upon the cut-off of 120-260 mg/dL total cholesterol) is significantly related to peripheral vascular disease and coronary heart disease, and it has become a significant clinical parameter. [5][6][7] Therefore, it is necessary to have a constant monitoring of cholesterol for medical screening or diagnosis.…”

Section: Introductionmentioning

confidence: 99%

<p>A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol</p>

Zhu¹,

Ye²,

Fan³

et al. 2019

IJN

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ObjectiveThis study aimed to construct a biosensor using Au nanoparticles (Au NPs) and reduced graphene-polyamide-amine-ferrocene (rGO-PAMAM-Fc) nanomaterials designed for rapid and sensitive detection of cholesterol.Materials and methodsIn this study, a highly sensitive biosensor based on Au NPs/ rGO-PAMAM-Fc nanomaterials was manufactured for detection of cholesterol. The rGO-PAMAM-Fc and Au NPs were modified on the surface of the electrode and then coated with cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) to develop the ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor.ResultsThe capability of rGO-PAMAM-Fc nanomaterials in fabricating a more efficient biosensor was validated through stability, selectivity and reproducibility checks. Under optimal conditions, the newly developed biosensor showed a linear relationship with logarithm of cholesterol concentration from 0.0004 to 15.36 mM (R2=0.9986), and a low detection limit of 2 nM was obtained at the signal/noise ratio of 3.ConclusionThe ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor was successfully applied for the measurement of cholesterol in human serum, which implies that the biosensor has a potential application in clinical diagnostics.

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“…Commonly, cholesterol biosensing is performed with the aid of cholesterol oxidase immobilized on a suitable electrode. [9][10][11][12][13] It is worth noting that H 2 O 2 may be also reduced at the surface of electrodes, [14,15] which may also be used as transduction reaction in a biosensor. Both the recognition event and biochemical oxidation of cholesterol have to be transduced into an electrical signal, which is achieved by including an adequate hydrogen peroxide electrocatalyst at the interface.…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, there are various inorganic compounds that have been shown to perform well in H 2 O 2 electrooxidation, and therefore have been incorporated in varied biosensors. [9][10][11][12][13] It is worth noting that H 2 O 2 may be also reduced at the surface of electrodes, [14,15] which may also be used as transduction reaction in a biosensor. In general, positive applied potentials favor H 2 O 2 oxidation, while negative potentials favor its reduction.…”

Section: Introductionmentioning

confidence: 99%

Bimetallic Co²⁺ and Mn²⁺ Hexacyanoferrate for Hydrogen Peroxide Electrooxidation and Its Application in a Highly Sensitive Cholesterol Biosensor

et al. 2019

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In this work, we report the synthesis and characterization of a bimetallic Prussian blue analogue containing Co and Mn as outer-sphere metals (CoMnHCF). The material was a solid solution and its characterization revealed a chemical composition of Co 2.05 Mn 0.95 [Fe(CN) 6 ] 2 · 12H 2 O. The electrochemistry of this novel material showed the existence of two redox waves displaying quasi-reversible kinetics, as expressed by the larger peak-to-peak separation upon increasing the potential sweep rate. Interestingly, the CoMnHCF solid exhibited high electrocatalytic activity for the oxidation of hydrogen peroxide, thus representing an appealing scaffold for the construction of biosensors. As a proof of concept, cholesterol oxidase was immobilized at the electrode surface by using a sol-gel method, and the cyclic voltammograms were recorded at increasing concentrations of cholesterol. The biosensor showed a detection limit of 30 μM and two linear ranges with excellent sensitivity of 385 mA cm À 2 M À 1 between 50 and 150 μM, and an adequate sensitivity of 80 mA cm À 2 M À 1 between 150 and 1 mM. To the best of our knowledge, this is the first biosensor application of a pre-synthesized bimetallic hexacyanometallate, thus exploiting its potential as an H 2 O 2 electrooxidation catalyst.

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Nanomaterials towards fabrication of cholesterol biosensors: Key roles and design approaches

Cited by 105 publications

References 131 publications

On the Accuracy of In Vivo Ethanol and Acetaldehyde Monitoring, a Key Tile in the Puzzle of Acetaldehyde as a Neuroactive Agent

On the Accuracy of In Vivo Ethanol and Acetaldehyde Monitoring, a Key Tile in the Puzzle of Acetaldehyde as a Neuroactive Agent

<p>A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol</p>

Bimetallic Co²⁺ and Mn²⁺ Hexacyanoferrate for Hydrogen Peroxide Electrooxidation and Its Application in a Highly Sensitive Cholesterol Biosensor

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Nanomaterials towards fabrication of cholesterol biosensors: Key roles and design approaches

Cited by 105 publications

References 131 publications

On the Accuracy of In Vivo Ethanol and Acetaldehyde Monitoring, a Key Tile in the Puzzle of Acetaldehyde as a Neuroactive Agent

On the Accuracy of In Vivo Ethanol and Acetaldehyde Monitoring, a Key Tile in the Puzzle of Acetaldehyde as a Neuroactive Agent

<p>A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol</p>

Bimetallic Co2+ and Mn2+ Hexacyanoferrate for Hydrogen Peroxide Electrooxidation and Its Application in a Highly Sensitive Cholesterol Biosensor

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Bimetallic Co²⁺ and Mn²⁺ Hexacyanoferrate for Hydrogen Peroxide Electrooxidation and Its Application in a Highly Sensitive Cholesterol Biosensor