Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus

Kim, Soo‐Kyung; Oh, Yoon-Hee; Ko, Dae-Hyun; Sung, Heungsup; Oh, Heung‐Bum; Hwang, Sang‐Hyun

doi:10.3390/bios12090744

Cited by 15 publications

(6 citation statements)

References 46 publications

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“…A study explores the use of nanoparticles for the visual detection of amplified DNA for the diagnosis of hepatitis C virus. 104 The study successfully visualized amplified DNA using nanoparticles in PCR mixtures, achieving an LoD of 101 IU/mL and consistent detection with standard real-time PCR. Furthermore, a biosensor made of ZnO nanorods and gold nanoparticles has been developed for the detection of hepatitis C. The electrochemical biosensor is a selective, highly sensitive, reproducible, and stable.…”

Section: Biosensors For Infectious Diseasesmentioning

confidence: 74%

“…The assay detects sGP protein in serum, aiding in viral infection diagnosis, with a rapid protocol, portable device, and minimal training requirement, making it ideal for point‐of‐care testing. A study explores the use of nanoparticles for the visual detection of amplified DNA for the diagnosis of hepatitis C virus 104 . The study successfully visualized amplified DNA using nanoparticles in PCR mixtures, achieving an LoD of 101 IU/mL and consistent detection with standard real‐time PCR.…”

Section: A New Era Of Infectious Diseasementioning

confidence: 99%

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The importance, benefits, and future of nanobiosensors for infectious diseases

Dhahi,

Dafhalla,

Saad

et al. 2024

Biotech and App Biochem

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Infectious diseases, caused by pathogenic microorganisms such as bacteria, viruses, parasites, or fungi, are crucial for efficient disease management, reducing morbidity and mortality rates and controlling disease spread. Traditional laboratory‐based diagnostic methods face challenges such as high costs, time consumption, and a lack of trained personnel in resource‐poor settings. Diagnostic biosensors have gained momentum as a potential solution, offering advantages such as low cost, high sensitivity, ease of use, and portability. Nanobiosensors are a promising tool for detecting and diagnosing infectious diseases such as coronavirus disease, human immunodeficiency virus, and hepatitis. These sensors use nanostructured carbon nanotubes, graphene, and nanoparticles to detect specific biomarkers or pathogens. They operate through mechanisms like the lateral flow test platform, where a sample containing the biomarker or pathogen is applied to a test strip. If present, the sample binds to specific recognition probes on the strip, indicating a positive result. This binding event is visualized through a colored line. This review discusses the importance, benefits, and potential of nanobiosensors in detecting infectious diseases.

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Section: Biosensors For Infectious Diseasesmentioning

confidence: 74%

Section: A New Era Of Infectious Diseasementioning

confidence: 99%

The importance, benefits, and future of nanobiosensors for infectious diseases

Dhahi,

Dafhalla,

Saad

et al. 2024

Biotech and App Biochem

View full text Add to dashboard Cite

show abstract

“…Attachment is followed by viral entry into the cell, a process followed by replication. DNA viruses use the cell’s proteins and enzymes to copy their DNA, while RNA viruses use their RNA as a template for replication [ 11 ]. Viral nucleic material directs the host cell to synthesize viral enzymes and capsid proteins, resulting in the assembly of new virions [ 12 ].…”

Section: Infectious Diseasesmentioning

confidence: 99%

Nanotechnology-Based Diagnostics for Diseases Prevalent in Developing Countries: Current Advances in Point-of-Care Tests

et al. 2023

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The introduction of point-of-care testing (POCT) has revolutionized medical testing by allowing for simple tests to be conducted near the patient’s care point, rather than being confined to a medical laboratory. This has been especially beneficial for developing countries with limited infrastructure, where testing often involves sending specimens off-site and waiting for hours or days for results. However, the development of POCT devices has been challenging, with simplicity, accuracy, and cost-effectiveness being key factors in making these tests feasible. Nanotechnology has played a crucial role in achieving this goal, by not only making the tests possible but also masking their complexity. In this article, recent developments in POCT devices that benefit from nanotechnology are discussed. Microfluidics and lab-on-a-chip technologies are highlighted as major drivers of point-of-care testing, particularly in infectious disease diagnosis. These technologies enable various bioassays to be used at the point of care. The article also addresses the challenges faced by these technological advances and interesting future trends. The benefits of point-of-care testing are significant, especially in developing countries where medical care is shifting towards prevention, early detection, and managing chronic conditions. Infectious disease tests at the point of care in low-income countries can lead to prompt treatment, preventing infections from spreading.

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“…Bacteriophages (bacteriophages) are viruses that infect and proliferate within bacteria by the usage of tail-spike proteins, which connect to particular receptors on the host cell [ 169 , 170 ]. Pathogens can be detected using electrochemical biosensors based on these identification components [ 155 , 171 , 172 , 173 ]. Bacteriophages’ appearance, selectivity, and structure are categorized into several classes.…”

Section: Pathogen Detection With Electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for Pathogen Detection: An Updated Review

et al. 2022

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Electrochemical biosensors are a family of biosensors that use an electrochemical transducer to perform their functions. In recent decades, many electrochemical biosensors have been created for pathogen detection. These biosensors for detecting infections have been comprehensively studied in terms of transduction elements, biorecognition components, and electrochemical methods. This review discusses the biorecognition components that may be used to identify pathogens. These include antibodies and aptamers. The integration of transducers and electrode changes in biosensor design is a major discussion topic. Pathogen detection methods can be categorized by sample preparation and secondary binding processes. Diagnostics in medicine, environmental monitoring, and biothreat detection can benefit from electrochemical biosensors to ensure food and water safety. Disposable and reusable biosensors for process monitoring, as well as multiplexed and conformal pathogen detection, are all included in this review. It is now possible to identify a wide range of diseases using biosensors that may be applied to food, bodily fluids, and even objects’ surfaces. The sensitivity of optical techniques may be superior to electrochemical approaches, but optical methods are prohibitively expensive and challenging for most end users to utilize. On the other hand, electrochemical approaches are simpler to use, but their efficacy in identifying infections is still far from satisfactory.

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Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus

Cited by 15 publications

References 46 publications

The importance, benefits, and future of nanobiosensors for infectious diseases

The importance, benefits, and future of nanobiosensors for infectious diseases

Nanotechnology-Based Diagnostics for Diseases Prevalent in Developing Countries: Current Advances in Point-of-Care Tests

Electrochemical Biosensors for Pathogen Detection: An Updated Review

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