Nanoparticle-Enabled Combination Therapy Showed Superior Activity against Multi-Drug Resistant Bacterial Pathogens in Comparison to Free Drugs

Brar, Amarpreet; Majumder, Satwik; Navarro, Maria Zardon; Benoit‐Biancamano, Marie‐Odile; Ronholm, Jennifer; George, Saji

doi:10.3390/nano12132179

Cited by 17 publications

(14 citation statements)

References 54 publications

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“…Some fine particles can be seen on the surface of HMSS after functionalization by CIT, which slightly increases its surface roughness. This discovery demonstrates that the synthesis of M-NH2-CIT@CIT does not affect the overall morphology and mesoporous structure of HMSS, indicating the high stability of hollow mesoporous silica spheres [31,32]. This finding is consistent with the literature [33].…”

Section: Characterization Resultssupporting

confidence: 90%

Utilizing Imine Bonds to Create a Self-Gated Mesoporous Silica Material with Controlled Release and Antimicrobial Properties

et al. 2023

Nanomaterials

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In recent years, silica nanomaterials have been widely studied as carriers in the field of antibacterial activity in food. Therefore, it is a promising but challenging proposition to construct responsive antibacterial materials with food safety and controllable release capabilities using silica nanomaterials. In this paper, a pH-responsive self-gated antibacterial material is reported, which uses mesoporous silica nanomaterials as a carrier and achieves self-gating of the antibacterial agent through pH-sensitive imine bonds. This is the first study in the field of food antibacterial materials to achieve self-gating through the chemical bond of the antibacterial material itself. The prepared antibacterial material can effectively sense changes in pH values caused by the growth of foodborne pathogens and choose whether to release antibacterial substances and at what rate. The development of this antibacterial material does not introduce other components, ensuring food safety. In addition, carrying mesoporous silica nanomaterials can also effectively enhance the inhibitory ability of the active substance.

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Section: Characterization Resultssupporting

confidence: 90%

Utilizing Imine Bonds to Create a Self-Gated Mesoporous Silica Material with Controlled Release and Antimicrobial Properties

et al. 2023

Nanomaterials

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“…Activities of the efflux pump and ß-lactamase enzyme in the isolates were determined as phenotypic characteristics of ABR. The efflux pump activities in the isolates were assessed following a pre-established protocol [ 56 ]. Briefly, bacterial cells cultured overnight in MHB were washed twice in phosphate-buffered saline (PBS) (1X) and adjusted to 1.0 McFarland standard (approximately 3 × 10 8 cfu/mL) using DensiCHEK plus (BioMerieux, USA)).…”

Section: Methodsmentioning

confidence: 99%

“…The Nitrocefin assay was performed to determine the ß-lactamase enzyme activity [ 56 ]. For this, isolates grown overnight in MHB were adjusted to McFarland 1.0.…”

Section: Methodsmentioning

confidence: 99%

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Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity

et al. 2023

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Background Staphylococcus aureus is one of the prevalent etiological agents of contagious bovine mastitis, causing a significant economic burden on the global dairy industry. Given the emergence of antibiotic resistance (ABR) and possible zoonotic spillovers, S aureus from mastitic cattle pose threat to both veterinary and public health. Therefore, assessment of their ABR status and pathogenic translation in human infection models is crucial. Results In this study, 43 S. aureus isolates associated with bovine mastitis obtained from four different Canadian provinces (Alberta, Ontario, Quebec, and Atlantic provinces) were tested for ABR and virulence through phenotypic and genotypic profiling. All 43 isolates exhibited crucial virulence characteristics such as hemolysis, and biofilm formation, and six isolates from ST151, ST352, and ST8 categories showed ABR. Genes associated with ABR (tetK, tetM, aac6’, norA, norB, lmrS, blaR, blaZ, etc.), toxin production (hla, hlab, lukD, etc.), adherence (fmbA, fnbB, clfA, clfB, icaABCD, etc.), and host immune invasion (spa, sbi, cap, adsA, etc.) were identified by analyzing whole-genome sequences. Although none of the isolates possessed human adaptation genes, both groups of ABR and antibiotic-susceptible isolates demonstrated intracellular invasion, colonization, infection, and death of human intestinal epithelial cells (Caco-2), and Caenorhabditis elegans. Notably, the susceptibilities of S. aureus towards antibiotics such as streptomycin, kanamycin, and ampicillin were altered when the bacteria were internalized in Caco-2 cells and C. elegans. Meanwhile, tetracycline, chloramphenicol, and ceftiofur were comparatively more effective with ≤ 2.5 log10 reductions of intracellular S. aureus. Conclusions This study demonstrated the potential of S. aureus isolated from mastitis cows to possess virulence characteristics enabling invasion of intestinal cells thus calling for developing therapeutics capable of targeting drug-resistant intracellular pathogens for effective disease management.

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“…AMR-related issues were addressed in two papers of the Special Issue. Brar et al developed nano-encapsulated drugs consisting of chitosan and silica NPs containing tetracycline and chlorpromazine [ 7 ]. The encapsulation of the antibiotic drug and efflux pump inhibiting agent into NPs improved their antibacterial efficacy due to targeting AMR mechanisms of pathogenic bacteria and reducing intracellular pathogen load.…”

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confidence: 99%

Nanomaterials and Microorganisms: From Green Synthesis to Antibacterial Applications in Medicine and Agriculture

Mortimer

Kahru

2022

Nanomaterials

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Nanoparticle-Enabled Combination Therapy Showed Superior Activity against Multi-Drug Resistant Bacterial Pathogens in Comparison to Free Drugs

Cited by 17 publications

References 54 publications

Utilizing Imine Bonds to Create a Self-Gated Mesoporous Silica Material with Controlled Release and Antimicrobial Properties

Utilizing Imine Bonds to Create a Self-Gated Mesoporous Silica Material with Controlled Release and Antimicrobial Properties

Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity

Nanomaterials and Microorganisms: From Green Synthesis to Antibacterial Applications in Medicine and Agriculture

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