Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking

Abstract

Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies due to their ability to migrate to damaged tissue without inducing immune reaction. Many techniques have been developed to trace MSCs and their differentiation efficacy; however, all of these methods have limitations. Conjugated polymer based water-dispersible nanoparticles (CPN) represent a new class of probes because they offer high brightness, improved photostability, high fluorescent quantum yield, and noncytotoxicity comparing to conventional dyes and quantum dots. We aimed to use this tool for tracing MSCs' fate in vitro and in vivo. MSC marker expression, survival, and differentiation capacity were assessed upon CPN treatment. Our results showed that after CPN labeling, MSC markers did not change and significant number of cells were found to be viable as revealed by MTT. Fluorescent signals were retained for 3 weeks after they were differentiated into osteocytes, adipocytes, and chondrocytes in vitro. We also showed that the labeled MSCs migrated to the site of injury and retained their labels in an in vivo liver regeneration model. The utilization of nanoparticle could be a promising tool for the tracking of MSCs in vivo and in vitro and therefore can be a useful tool to understand differentiation and homing mechanisms of MSCs.

PNIPAAM modified mesoporous hydroxyapatite for sustained osteogenic drug release and promoting cell attachment

“…Then the culture medium was changed every three days. After two passages, cells were used in the whole study [39]. …”

Molecular Imaging of Stem Cell Transplantation for Liver Diseases: Monitoring, Clinical Translation, and Theranostics

“…The results showed that the labeled mesenchymal stem cells (MSCs) migrated to the liver and retained their labels in an in vivo liver regeneration model. These studies demonstrated that the utilization of florescence labeling could be a promising tool for the tracking of stem cells transplanted in liver to understand differentiation and homing mechanisms [28]. Wang et al investigated upconversion nanoparticles- (UCNPs-) labeled mouse MSCs intravenous transplanted into mice and imaged using an in vivo upconversion luminescence (UCL) imaging system, observing the translocation of MSCs from lung where they initially accumulated, to liver [29].…”

Novel chemotherapeutics and other therapies for treating high-grade glioma

“…LRP-1 enables transcytosis of ligands across the BBB, and drugs that exploit this receptor, GRN1005 and ANG1005, have been well-tolerated in HGG and effective in crossing of the BBB and are currently being studied in recurrent GBM [129].…”