Nanoparticle-mediated dsRNA delivery for precision insect pest control: a comprehensive review

Arjunan, Nareshkumar; Thiruvengadam, Venkatesan; Sushil, SN

doi:10.1007/s11033-023-09187-6

Cited by 6 publications

(1 citation statement)

References 165 publications

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“…But also here, the results are not consistent, and the effects can be gene-specific [47]. The potential of nanoparticles for RNAi-based insect management, including different nanomaterials, nanoparticle formulations, and their effect on different insect species, has recently been reviewed elsewhere [74][75][76]. One should consider that RNAi technology, when incorporating additional components formulated with the RNA, might be subject to additional regulatory frameworks akin to those for chemicals, potentially losing the regulatory advantages typically associated with RNA-only biologicals.…”

Section: Discussionmentioning

confidence: 84%

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Figueiredo Prates,

Fiebig,

Schlosser

et al. 2024

IJMS

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In this study, we report the complexities and challenges associated with achieving robust RNA interference (RNAi)-mediated gene knockdown in the mosquitoes Aedes aegypti and Aedes albopictus, a pivotal approach for genetic analysis and vector control. Despite RNAi’s potential for species-specific gene targeting, our independent efforts to establish oral delivery of RNAi for identifying genes critical for mosquito development and fitness encountered significant challenges, failing to reproduce previously reported potent RNAi effects. We independently evaluated a range of RNAi-inducing molecules (siRNAs, shRNAs, and dsRNAs) and administration methods (oral delivery, immersion, and microinjection) in three different laboratories. We also tested various mosquito strains and utilized microorganisms for RNA delivery. Our results reveal a pronounced inconsistency in RNAi efficacy, characterized by minimal effects on larval survival and gene expression levels in most instances despite strong published effects for the tested targets. One or multiple factors, including RNase activity in the gut, the cellular internalization and processing of RNA molecules, and the systemic dissemination of the RNAi signal, could be involved in this variability, all of which are barely understood in mosquitoes. The challenges identified in this study highlight the necessity for additional research into the underlying mechanisms of mosquito RNAi to develop more robust RNAi-based methodologies. Our findings emphasize the intricacies of RNAi application in mosquitoes, which present a substantial barrier to its utilization in genetic control strategies.

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Section: Discussionmentioning

confidence: 84%

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Figueiredo Prates,

Fiebig,

Schlosser

et al. 2024

IJMS

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Characterization of an RR‐2 cuticle protein DcCP8 and its potential application based on SPc nanoparticle‐wrapped dsRNA in Diaphorina citri

Lu,

Xia,

Zhang

et al. 2024

Pest Management Science

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BACKGROUNDThe insect cuticle consists of chitin fibers and a protein matrix, which plays an important role in protecting the body from invasion of various pathogens and prevents water loss. Periodic synthesis and degradation of the cuticle is required for the growth and development of insects. Key genes involved in cuticle formation have long been considered a potential target for pest control.RESULTSIn this study, a member of the RR‐2 subfamily of cuticular protein 8 (DcCP8) was identified from the Diaphorina citri genome database. Immunofluorescence analysis suggested that DcCP8 was mainly located in the Diaphorina citri exocuticle and can be induced to up‐regulate 12 h following 20‐hydroxyecdysone (20E) treatment. Silencing of DcCP8 by RNA interference (RNAi) significantly disrupted the metamorphosis to the adult stage, and improved the permeability of the cuticle. Transmission electron microscopy (TEM) analysis revealed that the synthesis of the exocuticle was impressed after silencing of DcCP8. Furthermore, the recombinant DcCP8 protein exhibited chitin‐binding properties in vitro, down‐regulation of DcCP8 significantly inhibited expression levels of chitin metabolism‐related genes. Additionally, a sprayable RNAi method targeting DcCP8 based on star polycation (SPc) nanoparticles‐wrapped double‐stranded RNA (dsRNA) significantly increased Diaphorina citri mortality. Transcriptome sequencing further confirmed that genes associated with the endocytic pathway and immune response were up‐regulated in Diaphorina citri after SPc treatment.CONCLUSIONSThe current study indicated that DcCP8 is critical for the formation of Diaphorina citri exocuticles, and lays a foundation for Diaphorina citri control based on large‐scale dsRNA nanoparticles. © 2024 Society of Chemical Industry.

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RNA interference (RNAi) mediated technique for combating plant diseases: Harnessing nanoparticles for effective delivery and enhanced efficacy

Verma,

Modgil

2024

Plant Cell Tiss Organ Cult

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Nanoparticle-mediated dsRNA delivery for precision insect pest control: a comprehensive review

Cited by 6 publications

References 165 publications

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Characterization of an RR‐2 cuticle protein DcCP8 and its potential application based on SPc nanoparticle‐wrapped dsRNA in Diaphorina citri

RNA interference (RNAi) mediated technique for combating plant diseases: Harnessing nanoparticles for effective delivery and enhanced efficacy

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