Nanoparticle mediated PPARγ gene delivery on dental implants improves osseointegration via mitochondrial biogenesis in diabetes mellitus rat model

Lee, Younghee; Kim, Jeong‐Seok; Kim, Jieun; Jeon, Jongho; Park, Il‐Song; Yi, Ho-Keun

doi:10.1016/j.nano.2017.02.020

Cited by 43 publications

(32 citation statements)

References 29 publications

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“…Animal models provide relevant information about a variety of disorders in dentistry. The rat model has been widely used for studies in dentistry, including those which involve periodontal diseases, alveolar bone healing, tooth movement, osteogenesis, osseointegration, use of biomaterials, and altered occlusal loadings, for example (Okamoto and de Russo, 1973;Shimomoto et al, 2007;Walker et al, 2008;Wan et al, 2012;Xu et al, 2016;Zeng et al, 2016;Koutouzis et al, 2017;Lee et al, 2017;Hassumi et al, 2018;Palin et al, 2018;Uslu et al, 2018;Wang et al, 2018). Besides that, tooth extraction, inclosing incisor extraction, has been considered as a classical model of preclinical studies and has been applied in many studies with different aims in the last years (Avivi-Arber et al, 2010;Shoji et al, 2010;Boonyagul et al, 2014;Curra et al, 2016;Hassumi et al, 2018;de Oliveira Puttini et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

β-catenin and Its Relation to Alveolar Bone Mechanical Deformation – A Study Conducted in Rats With Tooth Extraction

et al. 2020

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Section: Discussionmentioning

confidence: 99%

β-catenin and Its Relation to Alveolar Bone Mechanical Deformation – A Study Conducted in Rats With Tooth Extraction

et al. 2020

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“…In addition, it is generally thought that inflammation exerts negative effects on osseointegration of Ti-based implants [38]. Lee et al [39] have recently demonstrated in the diabetes mellitus-induced rat model that PPARγ released from the corresponding cDNA introduced on titanium implant surfaces enhances osseointegration and implant longevity. However, there are limited data that supports such an application of PRP.…”

Section: Discussionmentioning

confidence: 99%

Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets

Tsujino

Takahashi²,

Watanabe

et al. 2019

Dentistry Journal

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Recent progress in the industrial development of dental implants has improved their surface bio-affinity, while clinical implantologists attempt to improve it through coating with various compounds, including platelet-rich plasma (PRP) in clinical settings. However, it is poorly understood how PRP acts on titanium surfaces. To validate this surface modification method and demonstrate how platelet-derived soluble biomolecules released from the activated adherent platelets act on plain, commercially pure-titanium (cp-Ti) plates, we evaluated the distribution of biomolecules by immunofluorescence. PPARγ, PDGF-B, and TGFβ1 were similarly released at immunofluorescence levels from activated adherent platelets, retained in the surrounding extra-platelet spaces for a while, and did not immediately diffuse away to distant spaces. Exogenously added CaCl2 augmented release and retention of those biomolecules along with activation and aggregation. Taken together with our previous data regarding platelet adhesion, these findings suggest that especially when treated with CaCl2, platelets immediately adhere on cp-Ti plates to release their stored biomolecules in the absence of plasma proteins and that these biomolecules do not diffuse away, but stay longer in extra-platelet spaces around the platelets by newly formed, immature fibrin fiber fragments. Consequently, these retained biomolecules are anticipated to cooperatively stabilize implants by stimulating alveolar bone regeneration and integration.

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“…More than three times as many DM patients have periodontitis than individuals without DM. A previous study found destruction of the periodontal tissue around the mandible and increased levels of inflammatory molecules in systemic DM-induced rats, suggesting that DM is involved in local periodontal tissue inflammation [ 9 ]. Therefore, periodontitis may be considered as a complication of DM [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Phelligridin D maintains the function of periodontal ligament cells through autophagy in glucose-induced oxidative stress

Kim

Lee

et al. 2020

J Periodontal Implant Sci

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Purpose The objective of this study was to investigate whether phelligridin D could reduce glucose-induced oxidative stress, attenuate the resulting inflammatory response, and restore the function of human periodontal ligament cells (HPDLCs). Methods Primary HPDLCs were isolated from healthy human teeth and cultured. To investigate the effect of phelligridin D on glucose-induced oxidative stress, HPDLCs were treated with phelligridin D, various concentrations of glucose, and glucose oxidase. Glucose-induced oxidative stress, inflammatory molecules, osteoblast differentiation, and mineralization of the HPDLCs were measured by hydrogen peroxide (H 2 O 2 ) generation, cellular viability, alkaline phosphatase (ALP) activity, alizarin red staining, and western blot analyses. Results Glucose-induced oxidative stress led to increased production of H 2 O 2 , with negative impacts on cellular viability, ALP activity, and calcium deposition in HPDLCs. Furthermore, HPDLCs under glucose-induced oxidative stress showed induction of inflammatory molecules (intercellular adhesion molecule-1, vascular cell adhesion protein-1, tumor necrosis factor-alpha, interleukin-1-beta) and disturbances of osteogenic differentiation (bone morphogenetic protein-2, and -7, runt-related transcription factor-2), cementogenesis (cementum protein-1), and autophagy-related molecules (autophagy related 5, light chain 3 I/II, beclin-1). Phelligridin D restored all these molecules and maintained the function of HPDLCs even under glucose-induced oxidative stress. Conclusions This study suggests that phelligridin D reduces the inflammation that results from glucose-induced oxidative stress and restores the function of HPDLCs (e.g., osteoblast differentiation) by upregulating autophagy.

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Nanoparticle mediated PPARγ gene delivery on dental implants improves osseointegration via mitochondrial biogenesis in diabetes mellitus rat model

Cited by 43 publications

References 29 publications

β-catenin and Its Relation to Alveolar Bone Mechanical Deformation – A Study Conducted in Rats With Tooth Extraction

β-catenin and Its Relation to Alveolar Bone Mechanical Deformation – A Study Conducted in Rats With Tooth Extraction

Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets

Phelligridin D maintains the function of periodontal ligament cells through autophagy in glucose-induced oxidative stress

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