Nanoparticle physicochemical properties determine the activation of intracellular complement

Ilinskaya, Anna; Shah, Avani; Enciso, Alan E.; Chan, King C.; Kaczmarczyk, Jan; Blonder, Josip; Simanek, Eric E.; Dobrovolskaia, Marina A.

doi:10.1016/j.nano.2019.02.002

Cited by 25 publications

(21 citation statements)

References 46 publications

(63 reference statements)

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“…Nanoscale materials have long been the focus of researchers and materials scientists. The reduction in particle size from micro to nano has led to the emergence of specific properties such as hardness, high electrical conductivity, increased surface-to-volume ratio, high reactivity, and altered biological activity (Ilinskaya et al, 2019). Such properties enable the particles to interact with microbes, bacterial membranes, and cancerous cells more meritoriously.…”

Section: Research Articlementioning

confidence: 99%

Smaller Copper Oxide Nanoparticles have More Biological Effects Versus Breast Cancer and Nosocomial Infections Bacteria

Abbasi

Ghorban

Nojoomi

et al. 2021

Asian Pac J Cancer Prev

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Background and Objectives: Despite promising successes in developing new drugs and pharmaceutical biotechnology, infectious diseases and cancer are still the principal causes of mortality and morbidity globally. Therefore, finding effective ways to deal with these pathogens and cancers is critical. Metal nanoparticles are one of the new strategies to combat bacteria and cancers. Methods: We examined the antimicrobial activity of 30 and 60 nm copper oxide nanoparticles (CuO-NPs) against Acinetobacter baumannii and Staphylococcus epidermidis bacteria responsible for nosocomial infections in standard and clinical strains and anti-cancer activity against 4T1 cell line as malignancy breast cancer cells. Synthesis of CuO-NPs was performed by a one-step reduction method and confirmed by DLS and TEM microscopy at 30 and 60 nm sizes. The antibacterial and anti-cancer activities of the nanoparticles were then investigated against the aforementioned bacteria and breast cancer. Results: Using disk, well, MIC, MBC methods, and viability/bacterial growth assay, 30 nm CuO NPs were found to have more antibacterial activity on standard and clinical strains than 60 nm CuO NPs. On the other hand, using MTT, apoptosis, and gene expression method, 30 nm nanoparticles were found to have more anti-cancer potential than 60 nm CuO NPs. Conclusions: Our findings implicate CuO-NPs to possess antimicrobial and anti-cancer effects and more significant potential in smaller sizes, suggesting their pharmaceutical and biomedical capacity.

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Section: Research Articlementioning

confidence: 99%

Smaller Copper Oxide Nanoparticles have More Biological Effects Versus Breast Cancer and Nosocomial Infections Bacteria

Abbasi

Ghorban

Nojoomi

et al. 2021

Asian Pac J Cancer Prev

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“…pre-clinically confirming in vitro (potentially followed by in vivo ) the anticipated consequences of quality-related differences ( 32 );…”

Section: Relevance Of Clinical Decisions In Regulatory Paths and Mandmentioning

confidence: 99%

Non-biological Complex Drugs (NBCDs): Complex Pharmaceuticals in Need of Individual Robust Clinical Assessment Before Any Therapeutic Equivalence Decision

et al. 2020

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Non-Biological Complex Drugs (NBCDs) are complex non-biological drugs comprised of large high molecular weight molecules and, often, nanoparticular structures (including liposomes and block-copolymer micelles). In the case of NBCDs, the entire complex is the active pharmaceutical ingredient and its properties cannot be fully characterized by physicochemical analysis. Moreover, the manufacturing process is fundamental in creating the correct originator product. The same is true for generic versions of the product. A recent appraisal of approval procedures for NBCDs “follow-on products” approved in Europe shows a diversity of regulatory pathways. In fact, three different abridged application procedures, under European legislation, were used: the generic application procedure of Article 10(1), the hybrid application procedure of Article 10(3), and the biosimilar application procedure of Article 10(4). Three informed consent applications via Article 10(c) from innovator companies of glatiramer acetate and sevelamer carbonate were submitted shortly after the approval of the first follow-on products. Furthermore, a number of “well-established use” applications [via Article 10(a)] were approved for iron sucrose and iron dextran complexes. In order to protect patients from the increased risks of NBCD products and NBCD follow-on products, two complementary approaches should be considered: (i) improving the regulatory procedures and their guidance documents within the pre-registration phase, and (ii) not considering interchangeability whenever clinical data is not available. With regards to the latter, the need for adequate safety and efficacy data might also include risk management programmes within post-approval pharmacovigilance actions. This, however, would depend on a risk appraisal that must be considered for individual medicinal products, based on the nature of the submitted relevant set of safety/efficacy data.

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“…Overall, these proteins “complement” antibodies in terms of promoting phagocytic clearance of foreign entities (pathogens). The binding of these proteins on the NPs may triggers the complement activation cascade by three different mechanisms (classical, alternative, lectin) ( Figure 6 ), depending on the physicochemical properties of NPs (size, morphology, surface patterns) and on the PEG composition and conformation ( Ilinskaya et al, 2019 ; Hamad et al, 2010 ; Pannuzzo et al, 2020 ). The classical and lectin pathway is initiated by the recognition of patterns on the NP surface by soluble mediators in the blood, such as antibodies, IgG or IgM, and sugars (mainly N-acetyl glucosamine and mannose), respectively.…”

Section: The Other Side Of the Coin: Adverse Effects Of Pegmentioning

confidence: 99%

Understanding the Role and Impact of Poly (Ethylene Glycol) (PEG) on Nanoparticle Formulation: Implications for COVID-19 Vaccines

Padín-González

Lancaster

Bottini

et al. 2022

Front. Bioeng. Biotechnol.

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Poly (ethylene glycol) (PEG) is a widely used polymer in a variety of consumer products and in medicine. PEGylation refers to the conjugation of PEG to drugs or nanoparticles to increase circulation time and reduce unwanted host responses. PEG is viewed as being well-tolerated, but previous studies have identified anti-PEG antibodies and so-called pseudoallergic reactions in certain individuals. The increased use of nanoparticles as contrast agents or in drug delivery, along with the introduction of mRNA vaccines encapsulated in PEGylated lipid nanoparticles has brought this issue to the fore. Thus, while these vaccines have proven to be remarkably effective, rare cases of anaphylaxis have been reported, and this has been tentatively ascribed to the PEGylated carriers, which may trigger complement activation in susceptible individuals. Here, we provide a general overview of the use of PEGylated nanoparticles for pharmaceutical applications, and we discuss the activation of the complement cascade that might be caused by PEGylated nanomedicines for a better understanding of these immunological adverse reactions.

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Nanoparticle physicochemical properties determine the activation of intracellular complement

Cited by 25 publications

References 46 publications

Smaller Copper Oxide Nanoparticles have More Biological Effects Versus Breast Cancer and Nosocomial Infections Bacteria

Smaller Copper Oxide Nanoparticles have More Biological Effects Versus Breast Cancer and Nosocomial Infections Bacteria

Non-biological Complex Drugs (NBCDs): Complex Pharmaceuticals in Need of Individual Robust Clinical Assessment Before Any Therapeutic Equivalence Decision

Understanding the Role and Impact of Poly (Ethylene Glycol) (PEG) on Nanoparticle Formulation: Implications for COVID-19 Vaccines

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