Nanoparticles as New Emerging Antibacterials: Potentials and Limitations

Husain, Fohad Mabood; Khan, Mohammad Saeed; Siddiqui, Saba; Khan, Altaf; Arshad, Muhammad; Alyousef, Abdullah A.; Rahman, Mashihur; Al‐Shabib, Nasser Abdulatif; Ahmad, Iqbal

doi:10.1007/978-981-13-9871-1_25

Cited by 5 publications

(2 citation statements)

References 112 publications

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“…In last few decades, there has been tremendous increase in the emergence and spread of drug resistance among bacterial pathogens. This antimicrobial resistance is now a global concern as it has become one of the major contributors in mortality and morbidity diseases [ 13 ]. Moreover, multiple drug-resistance also worsens the treatment of other infections associated with chronic illness such as diabetes and cancer by reducing the effectiveness of antibiotics [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

et al. 2021

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Green synthesis of metal nanoparticles using plant extracts as capping and reducing agents for the biomedical applications has received considerable attention. Moreover, emergence and spread of multidrug resistance among bacterial pathogens has become a major health concern and lookout for novel alternative effective drugs has gained momentum. In current study, we synthesized gold nanoparticles using the seed extract of Trachyspermum ammi (TA-AuNPs), assessed its efficacy against drug resistant biofilms of Listeria monocytogenes and Serratia marcescens, and evaluated its anticancer potential against HepG2 cancer cell lines. Microwave-assisted green synthesis of gold nanoparticles was carried out and characterization was done using UV-vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Most nanoparticles were observed as spherical and spheroidal with few anisotropies with an average crystalline size of 16.63 nm. Synthesized TA-AuNPs demonstrated significant biofilm inhibitory activity against L. monocytogenes (73%) as well as S. marcescens (81%). Exopolysaccharide (EPS), motility, and CSH, key elements that facilitate the formation and maintenance of biofilm were also inhibited significantly at the tested sub-minimum inhibitory concentrations (sub-MICs). Further, TA-AuNPs effectively obliterated preformed mature biofilms of S. marcescens and L. monocytogenes by 64% and 58%, respectively. Induction of intracellular ROS production in TA-AuNPs treated bacterial cells could be the plausible mechanism for the reduced biofilm formation in test pathogens. Administration of TA-AuNPs resulted in the arrest of cellular proliferation in a concentration-dependent manner. TA-AuNPs decrease the intracellular GSH in HepG2 cancer cell lines, cells become more prone to ROS generation, hence induce apoptosis. Thus, this work proposes a new eco-friendly and rapid approach for fabricating NPs which can be exploited for multifarious biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

et al. 2021

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“…Among diverse strategies developed to combat the threat of drug-resistant calcitrant biofilm, significant research has focused on the use of nanoparticles as novel antimicrobials [6]. Several metallic nanoparticles of silver and gold, metal oxide nanoparticles such as zinc oxide, iron oxide, tin oxide, titanium oxide, and carbon nanotubes have been reported to possess antimicrobial properties [6,7]. In addition to these, copper, copper oxide and silver oxide have attracted great attention due to their excellent antibacterial properties [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Bio-Inspired Facile Synthesis of Graphene-Based Nanocomposites: Elucidation of Antimicrobial and Biofilm Inhibitory Potential against Foodborne Pathogenic Bacteria

2020

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Herein, a new and simple biogenic method for the preparation of gold nanoparticles (AuNPs) and their reduced graphene oxide based nanocomposites (Au-RGO) by using microwave irradiation method for antimicrobial and biofilm inhibition against foodborne pathogenic bacteria was reported. X-ray diffraction (XRD), Raman, and transmission electron microscopy (TEM) analyses confirmed that the AuNPs with face centered cubic (FCC) structure were indeed anchored onto the RGO sheets. Ultraviolet-Vis (UV-VIS) spectrum showed a shifting and broadening of absorption peaks of AuNPs when attached on the surface of RGO sheets. The effect of sub-inhibitory concentrations of Au-RGO nanocomposites on biofilm formation in five foodborne pathogens was assessed. Au-RGO nanocomposites reduced the formation of biofilm by 75%, 78%, 68%, 80% and 79% in L. monocytogenes, MRSA, E. coli, S. marcescens and P. aeruginosa, respectively. Exopolysaccharides (EPS), a vital component of the biofilm was also inhibited significantly and pre-formed mature biofilms were also reduced considerably. Further, this study demonstrated that the reactive oxygen species (ROS) generation induced in bacterial cells as a result of Au-RGO treatment could be the plausible mechanism for biofilm inhibitory action. The tested concentrations were found non-toxic to human embryonic kidney cell lines (HEK-293). The investigation highlights the broad-spectrum biofilm inhibitory properties of Au-RGO nanocomposites that could be exploited in the food industry to prevent biofilm-based food contamination.

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Olea europaea mediated bioengineered biocompatible gold nanoparticles for antimicrobial, cytotoxic applications, and molecular docking study

Sellami

Khan

Amjad

et al. 2022

Journal of King Saud University - Science

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Nanoparticles as New Emerging Antibacterials: Potentials and Limitations

Cited by 5 publications

References 112 publications

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

Bio-Inspired Facile Synthesis of Graphene-Based Nanocomposites: Elucidation of Antimicrobial and Biofilm Inhibitory Potential against Foodborne Pathogenic Bacteria

Olea europaea mediated bioengineered biocompatible gold nanoparticles for antimicrobial, cytotoxic applications, and molecular docking study

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