Nanopipettes: a potential tool for DNA detection

Wang, Zhe; Liu, Yichong; Yu, Lei; Li, Yunchuan; Qian, Gongming; Chang, Shuai

doi:10.1039/c9an00633h

Cited by 18 publications

(13 citation statements)

References 97 publications

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“…We hope that this overview will be helpful for solving or minimizing some of the problems that hamper the usefulness of nanopore-based analytics of complex, real world samples. 275 We predict that the nanopore field will continue to expand the strategies for increasing the functionality of nanopores 300 and nanocapillaries [301][302][303] and that coatings will play an essential role in this development. We expect to witness an increase, both in the number of ways how coatings will be applied, and in the fine-tuning of their molecular composition.…”

Section: Discussionmentioning

confidence: 99%

Surface coatings for solid-state nanopores

2019

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Section: Discussionmentioning

confidence: 99%

Surface coatings for solid-state nanopores

2019

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“…In other words, if the current pulse can be resolved in real time and turn the applied potential off at arbitrary time, the number of translocation molecule could be controlled. With this technique coming true, one can expect that a precise number of substances could be injected into or extracted from a single cell at single molecular accuracy (Bulbul et al, 2018;Nadappuram et al, 2019;Wang et al, 2019).…”

Section: Nanopipette For Single-cell Samplingmentioning

confidence: 99%

“…Tay and Melosh have tried the tubular nanostructures for cargo delivery into cells (Tay and Melosh, 2019). More recently, nanopipette has shown great potential in DNA detection in vitro, it also demonstrates the ability to sample from nucleoplasm or cytoplasm (Wang et al, 2019). Meanwhile, applications of AFM and FluidFM technologies in molecular and cellular biology have also been reviewed recently, especially in the aspects, such as cellular morphology, cellular mechanics, and manipulation of a single cell (Amarouch et al, 2018;Li et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

Jia

Chen

2020

Front. Chem.

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The research in endogenous biomolecules from a single cell has grown rapidly in recent years since it is critical for dissecting and scrutinizing the complexity of heterogeneous tissues, especially under pathological conditions, and it is also of key importance to understand the biological processes and cellular responses to various perturbations without the limitation of population averaging. Although conventional techniques, such as micromanipulation or cell sorting methods, are already used along with subsequent molecular examinations, it remains a big challenge to develop new approaches to manipulate and directly extract small quantities of cytosol from single living cells. In this sense, nanostructure or nanomaterial may play a critical role in overcoming these challenges in cellular manipulation and extraction of very small quantities of cells, and provide a powerful alternative to conventional techniques. Since the nanostructures or nanomaterial could build channels between intracellular and extracellular components across cell membrane, through which cytosol could be pumped out and transferred to downstream analyses. In this review, we will first brief the traditional methods for single cell analyses, and then shift our focus to some most promising methods for single-cell sampling with nanostructures, such as glass nanopipette, nanostraw, carbon nanotube probes and other nanomaterial. In this context, particular attentions will be paid to their principles, preparations, operations, superiorities and drawbacks, and meanwhile the great potential of nano-materials for single-cell sampling will also be highlighted and prospected.

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“…More recently, novel configuration of sensing nanopores emerged based on nanopipettes [ 11 ] (see Figure 1 A). Those are made of glass with one electrode inside and the other in the surrounding medium.…”

Section: Introductionmentioning

confidence: 99%

“…Several reviews have focused on nanopores and their single molecule detection principle and applications [ 5 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. As mentioned previously, in most cases, the use of functional molecules brings new capabilities in term of detection, recognition, selectivity and even function to the nanopores.…”

Section: Introductionmentioning

confidence: 99%

Sensing with Nanopores and Aptamers: A Way Forward

Reynaud

Bouchet-Spinelli

Raillon

et al. 2020

Sensors

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In the 90s, the development of a novel single molecule technique based on nanopore sensing emerged. Preliminary improvements were based on the molecular or biological engineering of protein nanopores along with the use of nanotechnologies developed in the context of microelectronics. Since the last decade, the convergence between those two worlds has allowed for biomimetic approaches. In this respect, the combination of nanopores with aptamers, single-stranded oligonucleotides specifically selected towards molecular or cellular targets from an in vitro method, gained a lot of interest with potential applications for the single molecule detection and recognition in various domains like health, environment or security. The recent developments performed by combining nanopores and aptamers are highlighted in this review and some perspectives are drawn.

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Nanopipettes: a potential tool for DNA detection

Abstract: As a sub-group of solid-state nanopore, nanopipette shows great potential to develop into a platform for efficient and rapid DNA detection.

Cited by 18 publications

References 97 publications

Surface coatings for solid-state nanopores

Surface coatings for solid-state nanopores

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

Sensing with Nanopores and Aptamers: A Way Forward

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