Nanoplatforms attached Schiff bases by condensation method; Investigation of glucose oxidase enzyme as biocatalysts

Kayhan, Seda; Sarı, Nurşen; Nartop, Dılek

doi:10.3109/21691401.2013.856014

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…Similar to our previous results of the polymeric supports [27,28], it is considered that more reusability of particle capped double-ferrocene than other. The reusability results showed that the mediators (ferrocene) were attached strongly enough for use in the immobilization of reductase enzymes.…”

Section: Discussionsupporting

confidence: 73%

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Yetim

Özkan

Daniş

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

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A good novel double ferrocene-tagged nanomaterial is designed for glucose determination and its recycling stability. Double Ferrocene-tagged nanomaterial displays higher catalytic activity toward the glucose oxidation than non-ferrocene-tagged nanomaterial. In this study novel nanoparticles including double ferrocene, N-f2-[Bis(2-aminoethyl)amino]ethylgaminomethyl-polystyrene (2AEPS) and ferrocene aldehyde (Fc) have been synthesized by means of condensation and investigated the enzymatic properties of glucose oxidase enzyme (GOD) immobilized on there. Double ferrocene-tagged nanomaterial-GOD shows high reusability and storage capacity and fast incubation time determination of glucose. 2AEPSFc-GOD retains more than 15% of the initial activity after forty five successive cycles, which is a perfect performance.

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Section: Discussionsupporting

confidence: 73%

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Yetim

Özkan

Daniş

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

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“…Covalent immobilization efficiencies of enzymes on modified RHS Fourier transform infrared spectroscopy (FTIR) was used to analyze the chemical characteristics of the modified RHS [24,25] and the spectral data evidenced the covalent immobilization of BSA protein on GDA-linked RHS (Supplementary Figure S1). The immobilization efficiencies of the enzymes are shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Covalent immobilization of oxylipin biosynthetic enzymes on nanoporous rice husk silica for production of cis(+)-12-oxophytodienoic acid

Han²,

Cho

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Soybean lipoxygenase, recombinant rice allene oxide synthase-1 and rice allene oxide cyclase were covalently immobilized on nanoporous rice husk silica using two types of linkers: glutardialdehyde and polyethylene glycol. The immobilization efficiency achieved using glutardialdehyde-linked rice husk silica was higher than that achieved using polyethylene glycol-linked rice husk silica (50-92% and 25-50%, respectively). Immobilization on both types of matrices significantly decreased the specific activities of the immobilized enzymes. Solid-phase reaction yields of the enzymes were determined relative to the yields observed for the solution-phase reactions. Yields of the solid-phase reactions catalyzed by immobilized soybean lipoxygenase, rice allene oxide synthase-1, and rice allene oxide cyclase ranged from 50% to 230% and were dependent on both the enzymes and linkers used. Production of cis(+)-12-oxophytodienoic acid from α-linolenic acid by consecutive reactions using all three enzymes in a co-immobilization system resulted in 83.6% and 65.1% yields on glutardialdehyde-linked and epichlorohydrin-polyethylene glycol-linked rice husk silica, respectively. Our results suggest that immobilization of biosynthetic enzymes of the octadecanoid pathway on rice husk silica may be an efficient method for the in vitro production of oxylipins. Additionally, enzyme immobilizations on rice husk silica matrices may be more broadly applicable for producing physiologically important compounds in other biosynthetic pathways.

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“…[ 1 ] The azomethine group, –CHN–, has sp 2 hybridized orbital, and the hybridized orbital of nitrogen atom in the azomethine group is biologically significant which is involved in many different biological processes such as decarboxylation, transamination, and electron transfer. [ 2 ] Recently, metal complexes of Schiff base have been gaining interest in bioinorganic chemistry because many of these complexes provide biological synthetic models in understanding the structure of biomacromolecules such as DNA, protein, metalloprotein, and enzyme in biological systems. [ 3 ] Hemoglobin and hemocyanin have been modelled by many Schiff base complexes which naturally occurring dioxygen carriers and storage proteins contain a transition metal ion.…”

Section: Introductionmentioning

confidence: 99%

A new ternary Cu (II) complex with 4,7‐dimethyl‐1,10‐phenanthroline and NOO‐type tridentate Schiff base ligand: Synthesis, crystal structure, biomacromolecular interactions, and radical scavenging activities

İnci

2020

Applied Organom Chemis

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NOO‐type tridentate Schiff base, N‐salicylidene‐2‐aminobenzoic acid, (H2L), and its ternary Cu (II) complex containing H2L Schiff base and 4,7‐dimethyl‐1,10‐phenanthroline (4,7‐dmphen), [Cu(4,7‐dmphen)(H2L)]27H2O, have been synthesized and characterized by CHN analysis, ESI‐MS, FTIR, and single‐crystal X‐ray diffraction techniques. The interaction of alone H2L Schiff base ligand and ternary Cu (II) complex with biomacramolecules {calf thymus DNA (CT‐DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy. The experimental results indicate that H2L Schiff base ligand and ternary Cu (II) complex bind to CT‐DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between H2L Schiff base ligand and ternary Cu (II) complex with BSA possesses a static quenching process. Radical scavenging activity of H2L Schiff base ligand and ternary Cu (II) complex was measured in terms of EC50, using the DPPH and H2O2 methods. Biomacromolecule interactions and scavenging activity studies revealed that ternary Cu (II) complex yielded better results than H2L Schiff base ligand alone.

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Nanoplatforms attached Schiff bases by condensation method; Investigation of glucose oxidase enzyme as biocatalysts

Cited by 4 publications

References 17 publications

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Covalent immobilization of oxylipin biosynthetic enzymes on nanoporous rice husk silica for production of cis(+)-12-oxophytodienoic acid

A new ternary Cu (II) complex with 4,7‐dimethyl‐1,10‐phenanthroline and NOO‐type tridentate Schiff base ligand: Synthesis, crystal structure, biomacromolecular interactions, and radical scavenging activities

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