Nanopore-Based Fourth-Generation DNA Sequencing Technology

Feng, Yanxiao; Zhang, Yuechuan; Ying, Cuifeng; Wang, Deqiang; Du, Chunlei

doi:10.1016/j.gpb.2015.01.009

Cited by 373 publications

(290 citation statements)

References 98 publications

(167 reference statements)

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“…The latter is also impeded because the readouts from SGS are short. While a fourthgeneration sequencing technology, MinION (Oxford Nanopore Technologies), allows sequencing of a single molecule of a few thousand bases without the need for amplification (216), further reductions in sequencing error rates are needed before these technologies will be useful for epidemiological studies of mycobacteria.…”

Section: Methods Based On Nonrepetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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Section: Methods Based On Nonrepetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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“…Nanoengineering brings new expectations to the development of molecular techniques. Nanopore sequencing differs from the above-mentioned strategies by not reading the nucleotide sequences derived from the synthesis, but from subsequently cleaved single nucleotide matrix (14).…”

Section: Development Of Sequencing Techniquesmentioning

confidence: 99%

Development and application of genome sequencing in studies on poultry production traits and health

Dunisławska¹,

Sławińska²,

Siwek³

2018

Medycyna Weterynaryjna

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Summary Next-generation sequencing (NGS) is a novel method widely used in animal science and veterinary research.This technique revolutionized molecular biology and animal genetics research. The unquestionable advantage of NGS is an almost unlimited insight into genetic information. This review discusses the most important applications and achievements in poultry genomics available due to detailed sequence information. Here we present the development of sequencing methods and their further applications in poultry research. The chicken is an important livestock species and a model organism. It is the first non-mammalian amniote whose genome was sequenced by the International Chicken Genome Sequencing Consortium. Therefore, analysis of the chicken genome as a model organism and comparative analysis of genome reference plays an important role in current research. The detailed knowledge of the chicken genome position of genes associated with most important phenotypic traits will contribute to the development of molecular methods for the selection of animals.

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“…[16][17][18][19] Forensic DNA 20 , medical DNA 21 and other Clinical application regarding initial diagnosis and management in addition to considering the mechanisms of genetically related diseases can remain dependant. [18][19] There are three types of antigens responsible for the invasion process by this parasite to the tissue. These are mediated via major tachyzoite surface antigens which have different molecular weight "SAG1 (30 kDa), SAG2 (22 kDa) and SAG3 (43 kDa)".…”

Section: Introductionmentioning

confidence: 99%

Genotyping and Dna Sequencing Divergence of Toxoplasma Gondii Strains Associated With Rabbit Toxoplasmosis, Iraq

Shweash

Hayani

Hadeed

et al. 2022

Int J Life Sci Pharm Res

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The aim of this study is to determine the genetic patterns and sequencing of Toxoplasma gondii (T. gondii) isolated from humans and inoculated into rabbits to determine the effect of these strains on animals and the changes that occur in these strains. Therefore, DNA sequencing is necessary to apply with genetic developing for microorganisms to determine the relation between different organisms and how they are developed. Thirty-eight samples of congenital Toxoplasmosis were collected from the placenta of women who have had abortion and after their development were inoculated into rabbits. Seventeen samples of whole blood from rabbits were investigated. Iraqi human T. gondii were infected in 38 samples of rabbits, 17 samples out of 38 underwent gene sequence work from period 15 th September 2015 to 10 th may 2016. Data from 38 samples using the Surface Antigen Glycoproteins (SAG2) gene locus according to geno typing indicated that 22 out of 38 was types II, while 8 samples were types I and 8 showed types III. The results showed that there was a significant effect by the congenital Toxoplasmosis, which was developed in the bodies of rabbits, different strains of I, II, III, taken from the placenta of women who have had abortion because they were infected with T. gondii.

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Nanopore-Based Fourth-Generation DNA Sequencing Technology

Cited by 373 publications

References 98 publications

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Development and application of genome sequencing in studies on poultry production traits and health

Genotyping and Dna Sequencing Divergence of Toxoplasma Gondii Strains Associated With Rabbit Toxoplasmosis, Iraq

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