Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO

Fraiture, Marie‐Alice; Saltykova, Assia; Hoffman, Stefan; Winand, Raf; Deforce, Dieter; Vanneste, Kevin; Keersmaecker, Sigrid C. J. De

doi:10.1038/s41598-018-26259-x

Cited by 29 publications

(32 citation statements)

References 43 publications

(54 reference statements)

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“…Cattle (dairy and beef) and sheep belong to the ruminant category, with a unique digestive system that gives them the ability to obtain nutrients from forage crops by breaking down its cellulose content in a special stomach compartment using microbial actions. On the other hand, swine have a monogastric digestive system with an enzymatic stomach very similar to humans, and poultry have an avian digestive system without teeth to chew the feed (France et al, 2006). Cattle and sheep are herbivores, whereby their diet is mainly forage-based, while swine, poultry, and humans are omnivores, so they eat mostly grains and some plants (Flanders and Gillespie, 2015).…”

Section: Feed Safety Studiesmentioning

confidence: 99%

Safety Assessment of Genetically Modified Feed: Is There Any Difference From Food?

et al. 2019

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Section: Feed Safety Studiesmentioning

confidence: 99%

Safety Assessment of Genetically Modified Feed: Is There Any Difference From Food?

et al. 2019

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“…Because the unitary conductance of nanopores is generally high, conventional amplifiers can register any modulation of pore conductance and/or gating by the analyte of interest. The most prominent example of such a biotechnological application of conducting pores is the MinION device from Oxford Nanopore Technologies, which serves as a portable long-read DNA sequencing device (Fraiture et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Photolithographic Fabrication of Micro Apertures in Dry Film Polymer Sheets for Channel Recordings in Planar Lipid Bilayers

et al. 2019

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Planar lipid bilayers constitute a versatile method for measuring the activity of protein channels and pores on a single molecule level. Ongoing efforts attempt to tailor this method for detecting biomedically relevant target analytes or for highthroughput screening of drugs. To improve the mechanical stability of bilayer recordings, we use a thin-film epoxy resist ADEX as septum in free-standing vertical bilayers. Defined apertures with diameters between 30 µm and 100 µm were micro-fabricated by photolithography. The performance of these septa was tested by functional reconstitution of the K + channel Kcv NTS in lipid bilayers spanned over apertures in ADEX or Teflon films; the latter is conventionally used in bilayer recordings and serves as reference. We observe that the functional properties of the K + channel are identical in both materials while ADEX provides no advantage in terms of capacitance and signal-to-noise ratio. In contrast to Teflon, however, ADEX enables long-term experimental recordings while the stability of the lipid bilayer is not compromised by pipetting solutions in and out of the recording chamber. Combined with the fact that the ADEX films can be cleaned with acetone, our results suggest that ADEX carries great potential for multiplexing bilayer chambers in robust and reusable sensing devices.

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“…GM crops can be detected using several techniques (Cottenet et al, 2019;Dobnik et al, 2018;Fraiture et al, 2018). DNA-based approaches are more popular in detecting and quantifying GM crops than protein-based methods (Lipton et al, 2000), and real-time quantitative polymerase chain reaction (qPCR) is the standard in GMO analytics.…”

Section: Introductionmentioning

confidence: 99%

Capacity building for genetically modified organism (GMO) detection in West Africa: Identifying a circulating GMO maize variety in Mali

Traoré¹,

Diagne²,

Keita³

et al. 2019

Afr. J. Biotechnol.

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DNA-based methodology employing quantitative polymerase chain reaction (qPCR) has been successfully used to examine the incidence of genetically modified (GM) maize in Mali. This study aims to ascertain whether screening elements could also be used to detect GM maize. Fourteen maize varieties and one unknown dark color seeded variety from Mali were tested. DNA was extracted from three seeds of each variety. Three screening elements were used for qPCR amplification, the 35s promoter of the Cauliflower mosaic virus (CaMV), the nopaline synthase (NOS terminator) from Agrobacterium tumefaciens and the 35s promoter from the Figwort mosaic virus (FMV). The 14 varieties were negative for P35s CaMV (forward) and T-NOS (reverse) markers. In contrast, the unknown dark color seeded variety was positive with 94 bp PCR product. While, no DNA fragments were amplified using the FMV as the screening element. These data were supported by Ct values in which the 14 varieties had values above 50; whereas, the unknown variety showed values of 24.5 for P-35s-CaMV and 30 for the T-NOS. The study demonstrates the ability in detecting GM maize using screening elements and the usefulness of our laboratory in training and reinforcing regional concern about GMO circulation.

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Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO

Cited by 29 publications

References 43 publications

Safety Assessment of Genetically Modified Feed: Is There Any Difference From Food?

Safety Assessment of Genetically Modified Feed: Is There Any Difference From Food?

Photolithographic Fabrication of Micro Apertures in Dry Film Polymer Sheets for Channel Recordings in Planar Lipid Bilayers

Capacity building for genetically modified organism (GMO) detection in West Africa: Identifying a circulating GMO maize variety in Mali

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