Nanoscale characterization of collagen structural responses to in situ loading in rat Achilles tendons

Barreto, Isabella Silva; Pierantoni, M.; Hammerman, Malin; Törnquist, Elin; Cann, Sophie Le; Díaz, Ana; Engqvist, Jonas; Liebi, Marianne; Eliasson, Pernilla; Isaksson, Hanna

doi:10.1016/j.matbio.2022.11.006

Cited by 10 publications

(7 citation statements)

References 58 publications

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“…For each step, 10 SAXS measurements at 1.2 s intervals followed by 12 measurements at 25 s intervals were acquired. With this setup, the results are not significantly affected by radiation damage to the sample, as previously described [48] . Analysis of the 2D scattering patterns was performed using in-house codes in MATLAB [31] following our previously described protocols [48] .…”

Section: In Situ Loading Combined With Small-angle X-ray Scattering (...supporting

confidence: 59%

“…With this setup, the results are not significantly affected by radiation damage to the sample, as previously described [48] . Analysis of the 2D scattering patterns was performed using in-house codes in MATLAB [31] following our previously described protocols [48] . For more information about SAXS experiment and data analysis see Supporting information, Method S2, Figure S2 and Table S1 A.…”

Section: In Situ Loading Combined With Small-angle X-ray Scattering (...supporting

confidence: 59%

“…SAXS measurements were conducted at the coherent smallangle X-ray scattering beamline (cSAXS) at the Swiss Light Source (SLS), Paul Scherrer Institut (PSI), Switzerland using a similar protocol as previously developed by the authors [48] . Measurements were conducted using a beam energy of 12.4 keV (wavelength λ of ∼1 Å), an exposure time of 50 ms, a beam size of 150 ×125 μm 2 and a sample-detector distance of ∼7.14 m, enabling data acquisition in the q-range of ∼0.02-1.45 nm −1 .…”

Section: In Situ Loading Combined With Small-angle X-ray Scattering (...mentioning

confidence: 99%

“…At the nanoscale, SAXS can be used to probe the periodically arranged tropocollagen molecules inside the fibrils, and provide information about structural properties at the nanoscale [ 10 , 30 , 31 ]. SAXS, in combination with in situ mechanical loading, provides information on collagen fibril strain, strain heterogeneity, fibril organization, and degree of orientation in response to loading [32][33][34][35][36] . By performing SR-PhC-μCT, histology, SAXS and mechanical testing we have used a multimodal approach to investigate microstructural organization, collagen fibril structural response to in situ loading, biomechanical properties, and extracellular matrix protein content of rat Achilles tendons which were either unloaded (UL) by Botox injections in combination with ankle immobilization or were normally loaded (full loading, FL) through free cage activity.…”

Section: Introductionmentioning

confidence: 99%

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Multimodal and Multiscale Characterization Reveals How Tendon Structure and Mechanical Response are Altered by Reduced Loading

Pierantoni

Barreto

Hammerman

et al. 2023

Preprint

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Section: In Situ Loading Combined With Small-angle X-ray Scattering (...supporting

confidence: 59%

Section: In Situ Loading Combined With Small-angle X-ray Scattering (...supporting

confidence: 59%

Section: In Situ Loading Combined With Small-angle X-ray Scattering (...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multimodal and Multiscale Characterization Reveals How Tendon Structure and Mechanical Response are Altered by Reduced Loading

Pierantoni

Barreto

Hammerman

et al. 2023

Preprint

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“…However, the identification of material properties is complicated by unavoidable spatial heterogeneity in soft tissue deformation and damage [2, 19, 21, 24, 35]. To characterize true material properties, we must account for spatial heterogeneity in order to disentangle geometric effects from the material response.…”

Section: Introductionmentioning

confidence: 99%

A method for defining tissue injury criteria reveals ligament deformation thresholds are multimodal

Luetkemeyer

Neu

Calve

2023

Preprint

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Soft tissue injuries (such as ligament, tendon, and meniscus tears) are the result of extracellular matrix damage from excessive tissue stretching. Deformation thresholds for soft tissues, however, remain largely unknown due to a lack of methods that can measure and compare the spatially heterogeneous damage and deformation that occurs in these materials. Here, we propose a method for defining tissue injury criteria: multimodal strain limits for biological tissues analogous to yield criteria that exist for crystalline materials. Specifically, we developed a method for defining injury criteria for mechanically-driven fibrillar collagen denaturation in soft tissues, using regional multimodal deformation and damage data. We established this new method using the murine medial collateral ligament (MCL) as our model tissue. Our findings revealed that multiple modes of deformation contribute to collagen denaturation in the murine MCL, contrary to the common assumption that collagen damage is driven by strain in the fiber direction alone. Remarkably, our results indicated that hydrostatic strain, or volumetric expansion, may be the best predictor of mechanically-driven collagen denaturation in ligament tissue, suggesting crosslink-mediated stress transfer plays a role in molecular damage accumulation. This work demonstrates that collagen denaturation can be driven by multiple modes of deformation and provides a method for defining deformation thresholds, or injury criteria, from spatially heterogeneous data.

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In Situ Loading and Time‐Resolved Synchrotron‐Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof‐of‐Concept Study

Dejea,

Pierantoni,

Orozco

et al. 2024

Advanced Science

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Articular cartilage and meniscus transfer and distribute mechanical loads in the knee joint. Degeneration of these connective tissues occurs during the progression of knee osteoarthritis, which affects their composition, microstructure, and mechanical properties. A deeper understanding of disease progression can be obtained by studying them simultaneously. Time‐resolved synchrotron‐based X‐ray phase‐contrast tomography (SR‐PhC‐µCT) allows to capture the tissue dynamics. This proof‐of‐concept study presents a rheometer setup for simultaneous in situ unconfined compression and SR‐PhC‐µCT of connective knee tissues. The microstructural response of bovine cartilage (n = 16) and meniscus (n = 4) samples under axial continuously increased strain, or two steps of 15% strain (stress–relaxation) is studied. The chondrocyte distribution in cartilage and the collagen fiber orientation in the meniscus are assessed. Variations in chondrocyte density reveal an increase in the top 40% of the sample during loading, compared to the lower half. Meniscus collagen fibers reorient perpendicular to the loading direction during compression and partially redisperse during relaxation. Radiation damage, image repeatability, and image quality assessments show little to no effects on the results. In conclusion, this approach is highly promising for future studies of human knee tissues to understand their microstructure, mechanical response, and progression in degenerative diseases.

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Nanoscale characterization of collagen structural responses to in situ loading in rat Achilles tendons

Cited by 10 publications

References 58 publications

Multimodal and Multiscale Characterization Reveals How Tendon Structure and Mechanical Response are Altered by Reduced Loading

Multimodal and Multiscale Characterization Reveals How Tendon Structure and Mechanical Response are Altered by Reduced Loading

A method for defining tissue injury criteria reveals ligament deformation thresholds are multimodal

In Situ Loading and Time‐Resolved Synchrotron‐Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof‐of‐Concept Study

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