Nanoscale Electrical Potential and Roughness of a Calcium Phosphate Surface Promotes the Osteogenic Phenotype of Stromal Cells

Abstract

Mesenchymal stem cells (MSCs) and osteoblasts respond to the surface electrical charge and topography of biomaterials. This work focuses on the connection between the roughness of calcium phosphate (CP) surfaces and their electrical potential (EP) at the micro- and nanoscales and the possible role of these parameters in jointly affecting human MSC osteogenic differentiation and maturation in vitro. A microarc CP coating was deposited on titanium substrates and characterized at the micro- and nanoscale. Human adult adipose-derived MSCs (hAMSCs) or prenatal stromal cells from the human lung (HLPSCs) were cultured on the CP surface to estimate MSC behavior. The roughness, nonuniform charge polarity, and EP of CP microarc coatings on a titanium substrate were shown to affect the osteogenic differentiation and maturation of hAMSCs and HLPSCs in vitro. The surface EP induced by the negative charge increased with increasing surface roughness at the microscale. The surface relief at the nanoscale had an impact on the sign of the EP. Negative electrical charges were mainly located within the micro- and nanosockets of the coating surface, whereas positive charges were detected predominantly at the nanorelief peaks. HLPSCs located in the sockets of the CP surface expressed the osteoblastic markers osteocalcin and alkaline phosphatase. The CP multilevel topography induced charge polarity and an EP and overall promoted the osteoblast phenotype of HLPSCs. The negative sign of the EP and its magnitude at the micro- and nanosockets might be sensitive factors that can trigger osteoblastic differentiation and maturation of human stromal cells.

Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

“…The installation system consisted of a pulsed DC power supply, an electrolytic bath with a water-cooling system, two electrodes, and a computer to control the process. To synthesize the MAO coatings, the standard electrolyte contained 27 wt.% H 3 PO 4 , 7 wt.% CaCO 3 , 5 wt.% nanosized stoichiometric hydroxyapatite (HAp; Ca 10 (PO 4 ) 6 (OH) 2 ) and distilled water as a balance [ 28 ]. The obtained electrolyte suspension was an acidic medium with a pH of 1–2.…”

“…The mass of the experimental samples before and after MAO treatment was measured using a digital microanalytical balance (GR-202, A&D Company, Tokyo, Japan). The surface roughness was estimated by the average roughness ( R a ) using a contact profilometer (Hommel-Etamic T1000 Basic, Jenoptik, Jena, Germany) [ 31 ] because a strong linear correlation between R a , R z , and R max for microarc CaP coatings was identified [ 28 ]. The traverse length and rate of the measured profile were 6 mm and 0.5 mm/s, respectively.…”

Recent Advances in Manufacturing Innovative Stents

“…The surface can be modified in order to avoid or to reduce undesired corrosion able to disturb a stent integrity and its function [7] as well as to cause ion release, which leads to significant impact on the surrounding vascular cells. In [11], an innovative approach of electrical surface functionalization based on engineering the electrical charge on the stent surface has been reported. The influence of electrostatic factor on the cell attachment is demonstrated as important tool for manipulation of cell response directly associated with restenosis and thrombosis.…”

“…Some of the most used activation procedures involved chemical surface modifications, which can even be induced by physical treatments, such as plasma treatment [15][16][17]. Coated stents have been developed to improve the properties of BMS providing better biocompatibility, non-toxicity, suitable surface roughness, and surface free energy, regulating the ability of a stent surface to adsorb biological molecules and cells, ensuring chemical stability by regulating the corrosion rate [4] or providing In [11], an innovative approach of electrical surface functionalization based on engineering the electrical charge on the stent surface has been reported. The influence of electrostatic factor on the cell attachment is demonstrated as important tool for manipulation of cell response directly associated with restenosis and thrombosis.…”

Bone-Like Multilevel Calcium Phosphate Coating Modulates an Interaction of Mesenchymal Stem Cells and Tumor Cells

“…AND TUMOR CELLS 1 Malashchenko V.V., 1 Shunkin E.O., 1 Shupletsova V.V., 1 Khaziakhmatova O.G., 1 Yurova K.A., 1 Melashchenko E.S., 1 Litvinova L.S., 2,3 Khlusov I.A., 4 Komarova E.G., 4 Chebodaeva V.V., 4 SharkeevYu.P. 1 Leukemia initiation and progression are connected with a disfunction of health cell microenvironment and human multipotent mesenchymal stromal cells (hMMSCs), in particular. Modern biomaterial biocompatibility research focuses the biomaterial hierarchic effects on MMSC behavior (that occur at the nano-, micro-and macroscales) because MMSCs are the fundamental units that produce/regenerate bone tissue.…”