Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum

Jusík, Tomáš; Podešva, Pavel; Farka, Zdeněk; Kovás, David; Skládal, Petr; Foret, František

doi:10.1016/j.electacta.2015.12.009

Cited by 39 publications

(20 citation statements)

References 53 publications

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“…Some optical, colorimetric glucose sensing mechanisms based on noble metal nanoparticles have been presented in the literature, but most rely on pre-functionalization of particles or complementary use of enzymes or other catalytic reactions for the production of color signals, instead of using direct reactions between glucose and the precursor reagents, and there is yet to be such approach translated into a paper-based platform [ 22 , 23 , 24 , 25 , 26 , 27 ]. Nanostructured gold has also been applied in electrochemical sensing as a means of improving sensors catalytic capabilities, both in conjugation with GOx or for the development of non-enzymatic sensors [ 28 , 29 , 30 , 31 , 32 ]. However, these require more complex setups, both in terms of manufacture and use, hindering their portability and sustainability, when compared to a paper-based, colorimetric approach.…”

Section: Introductionmentioning

confidence: 99%

Paper-Based In-Situ Gold Nanoparticle Synthesis for Colorimetric, Non-Enzymatic Glucose Level Determination

et al. 2020

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Due to its properties, paper represents an alternative to perform point-of-care tests for colorimetric determination of glucose levels, providing simple, rapid, and inexpensive means of diagnosis. In this work, we report the development of a novel, rapid, disposable, inexpensive, enzyme-free, and colorimetric paper-based assay for glucose level determination. This sensing strategy is based on the synthesis of gold nanoparticles (AuNPs) by reduction of a gold salt precursor, in which glucose acts simultaneously as reducing and capping agent. This leads to a direct measurement of glucose without any enzymes or depending on the detection of intermediate products as in conventional enzymatic colorimetric methods. Firstly, we modelled the synthesis reaction of AuNPs to determine the optical, morphological, and kinetic properties and their manipulation for glucose sensing, by determining the influence of each of the reaction precursors towards the produced AuNPs, providing a guide for the manipulation of nucleation and growth. The adaptation of this synthesis into the developed paper platform was tested and calibrated using different standard solutions with physiological concentrations of glucose. The response of the colorimetric signals obtained with this paper-based platform showed a linear behavior until 20 mM, required for glycemic control in diabetes, using the Red × Value/Grey feature combination as a calibration metric, to describe the variations in color intensity and hue in the spot test zone. The colorimetric sensor revealed a detection limit of 0.65 mM, depending on calibration metric and sensitivity of 0.013 AU/mM for a linear sensitivity range from 1.25 to 20 mM, with high specificity for the determination of glucose in complex standards with other common reducing interferents and human serum.

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Section: Introductionmentioning

confidence: 99%

Paper-Based In-Situ Gold Nanoparticle Synthesis for Colorimetric, Non-Enzymatic Glucose Level Determination

et al. 2020

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“…Numerous papers have reported that nanostructured gold surfaces exhibit electro-catalytic activity towards oxidation of many organic species. Conversely, flat gold surfaces show poor or negligible catalytic properties [23,24]. The electro-catalyzed redox reactions by gold are highly pH dependent and are favored in alkali media, due to leading role of the negatively charged reaction intermediates.…”

Section: Introductionmentioning

confidence: 99%

“…Nanostructured gold and nanocomposites based gold electrodes have been prepared using a variety of methods [26][27][28][29][30]. Gold nanostructures onto graphene and carbon nanotubes [31,32] have been constructed by chemical etching [33], electrochemical deposition in hydrophilic gel templates-gelatin and agarose [23], electrochemical etching [34], de-alloying [35], electrochemical deposition using gelatin template [36]. It is also pointed out the advantages of carbon materials used as substrate which include the possibility of a variety of chemical functionalization, the absence of interfering metal ions, low cost, high electrical conductivity, and large amplitude of working potential [37].…”

Section: Introductionmentioning

confidence: 99%

Dewetted Gold Nanostructures onto Exfoliated Graphene Paper as High Efficient Glucose Sensor

et al. 2019

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Non-enzymatic electrochemical glucose sensing was obtained by gold nanostructures on graphene paper, produced by laser or thermal dewetting of 1.6 and 8 nm-thick Au layers, respectively. Nanosecond laser annealing produces spherical nanoparticles (AuNPs) through the molten-phase dewetting of the gold layer and simultaneous exfoliation of the graphene paper. The resulting composite electrodes were characterized by X-ray photoelectron spectroscopy, cyclic voltammetry, scanning electron microscopy, micro Raman spectroscopy and Rutherford back-scattering spectrometry. Laser dewetted electrode presents graphene nanoplatelets covered by spherical AuNPs. The sizes of AuNPs are in the range of 10–150 nm. A chemical shift in the XPS Au4f core-level of 0.25–0.3 eV suggests the occurrence of AuNPs oxidation, which are characterized by high stability under the electrochemical test. Thermal dewetting leads to electrodes characterized by faceted not oxidized gold structures. Glucose was detected in alkali media at potential of 0.15–0.17 V vs. saturated calomel electrode (SCE), in the concentration range of 2.5μM−30 mM, exploiting the peak corresponding to the oxidation of two electrons. Sensitivity of 1240 µA mM−1 cm−2, detection limit of 2.5 μM and quantifications limit of 20 μM were obtained with 8 nm gold equivalent thickness. The analytical performances are very promising and comparable to the actual state of art concerning gold based electrodes.

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“…But, they did not do any works on gold test strips nor any works on the effect of electrode design on glucose measurement. Zeng et al [4], Wang and Pamidi [5], Juſík et al [6], Nouira et al [7], Rafighi et al [8], and Ramanavicius et al [9] discussed gold nanoparticle deposition in carbon electrodes for enhanced performance. Hsu et al [10] further disclosed another biosensor electrode design of gold electrodes.…”

Section: Introductionmentioning

confidence: 99%

Design of Electrodes on Gold Test Strips for Enhanced Accuracy in Glucose Measurement

et al. 2019

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This paper reviews the practical process of designing the electrodes on electroless immersion gold-plated test strips for glucose measurement. We have found that great care must be taken on determining the width and length of the working and reference electrodes, to yield the optimum performance on electroless immersion gold-plated glucose test strips for glucose measurement. Preferably, a width of at least 0.6 mm and a length of at least 24 mm are recommended to prevent variance in electrode resistance during mass production of electroless immersion gold-plated glucose test strips, in order to enhance the accuracy of glucose measurement. This paper also recommended that, if two different geometric sizes of test strips are going to be used on the same glucose meter, both test strips must use different lengths and widths on the working and reference electrodes to yield the same electrical resistance and hence similar electrochemical performance.

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Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum

Cited by 39 publications

References 53 publications

Paper-Based In-Situ Gold Nanoparticle Synthesis for Colorimetric, Non-Enzymatic Glucose Level Determination

Paper-Based In-Situ Gold Nanoparticle Synthesis for Colorimetric, Non-Enzymatic Glucose Level Determination

Dewetted Gold Nanostructures onto Exfoliated Graphene Paper as High Efficient Glucose Sensor

Design of Electrodes on Gold Test Strips for Enhanced Accuracy in Glucose Measurement

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