Nanostructured Hematite Decorated with Gold Nanoparticles for Functionalization and Biocompatibility

Menezes, Lucivaldo R.; Sombrio, Guilherme; Costa, Claudia A.; Bronzato, Julia D.; Rodrigues, Tiago; Souza, J. A.; Nantes, Iseli L.

doi:10.1002/pssa.202070021

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…XPS spectrum of Au4f presented the typical sharp doublet peaks at 87.78 ± 0.03 eV and 84.08 ± 0.03 eV assigned respectively to Au 4f7/2 and Au 4f5/2 binding energies (BE) of nanoparticulated zero valent Au 0 (87.7 eV and 84.0 eV). [ 55 ]. The decomposition of the O1s XPS spectrum in Fig.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of bioluminescent gold nanoparticle–luciferase hybrid systems for technological applications

Belleti

Bevilaqua

Brito

et al. 2021

Photochem Photobiol Sci

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Graphic abstract Bioluminescent gold nanoparticles (AuNPs) were synthesized in situ using dithiol-terminated polyethylene glycol (PEG(SH) 2 ) as reducer and stabilizing agents. Hybrid Au/F 3 O 4 nanoparticles were also produced in a variation of synthesis, and both types of nanostructures had the polymer capping replaced by l -cysteine (Cys). The four types of nanoparticles, PEG(SH) 2 AuNPs, PEG(SH) 2 Au/F 3 O 4 NPs, CysAuNPs, and CysAu/F 3 O 4 NPs were associated with purified recombinant Pyrearinus termitilluminans green emitting click beetle luciferase (PyLuc) and Phrixotrix hirtus (RELuc) red-emitting railroad worm luciferase. Enzyme association with PEG(SH) 2 was also investigated as a control. Luciferases were chosen because they catalyze bioluminescent reactions used in a wide range of bioanalytical applications, including ATP assays, gene reporting, high-throughput screening, bioluminescence imaging, biosensors and other bioluminescence-based assays. The immobilization of PyLuc and RELuc promoted partial suppression of the enzyme luminescence activity in a functionalization-dependent way. Association of PyLuc and RELuc with AuNPs increased the enzyme operational stability in relation to the free enzyme, as evidenced by the luminescence intensity from 0 to 7 h after substrate addition. The stability of the immobilized enzymes was also functionalization-dependent and the association with CysAuNPs was the condition that combined more sustained luminescent activity with a low degree of luminescence quenching. The higher enzymatic stability and sustained luminescence of luciferases associated with nanoparticles may improve the applicability of bioluminescence for bioimaging and biosensing purposes. Supplementary Information The online version contains supplementary material available at 10.1007/s43630-021-00111-0.

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Section: Resultsmentioning

confidence: 99%

Synthesis of bioluminescent gold nanoparticle–luciferase hybrid systems for technological applications

Belleti

Bevilaqua

Brito

et al. 2021

Photochem Photobiol Sci

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“…Among them, gold nanoparticles (AuNPs) and carbon dots (CDs) have gained considerable attention due to their tunable optical properties, superior biocompatibility, and amenability to surface modification. 28,29 Indeed, several reports have shown that AuNPs can be utilized for optical detection, nanoenzyme catalysis, and medical treatment (reviewed in ref 23). 30 CDs have also exhibited potential for optical and electrochemical sensing.…”

mentioning

confidence: 99%

“…Recent advancements in nanotechnology have had positive impacts on the bioanalytical and biomedical fields. , For example, nanoparticles can now be used to enhance the physicochemical properties of molecular scaffolds via surface modification and functionalization. Among them, gold nanoparticles (AuNPs) and carbon dots (CDs) have gained considerable attention due to their tunable optical properties, superior biocompatibility, and amenability to surface modification. , Indeed, several reports have shown that AuNPs can be utilized for optical detection, nanoenzyme catalysis, and medical treatment (reviewed in ref ) . CDs have also exhibited potential for optical and electrochemical sensing.…”

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confidence: 99%

Versatile Gold-Coupled Te-Carbon Dots for Quantitative Monitoring and Efficient Scavenging of Superoxide Anions

Zhang

Wang

et al. 2021

Anal. Chem.

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The superoxide anion (O2 •–) is a reactive oxygen species (ROS) that functions as an important regulator of signal transduction in living systems. However, excess O2 •– can cause metabolic imbalances and oxidative damage inside cells. Quantitative detection and efficient scavenging of O2 •– are therefore critical for maintaining intracellular redox balance and homeostasis. In this work, a nanomaterial (Au-TeCD) composed of BSA-modified gold nanoparticles (AuNPs) complexed with tellurium-containing carbon dots (TeCDs) was constructed. The introduction of Au-TeCDs to solutions containing superoxide resulted in enhanced elimination of the anion, indicating that Au-TeCDs are able to scavenge O2 •– from the surrounding environment. Notably, the respective TeCD and AuNP components of the Au-TeCDs were found to emit fluorescence at 425 and 640 nm upon exposure to superoxide anions. This unique spectroscopic property of Au-TeCDs allowed levels of O2 •– in solution to be quantified using dual-fluorescence detection. The Au-TeCDs developed herein also exhibited low-cytotoxicity, versatile capabilities for in situ fluorescence imaging, and effective scavenging of O2 •– in living cells. Taken together, these results suggest that Au-TeCDs act as effective tools for monitoring superoxide concentrations in complex mixtures and may be developed as possible therapeutics designed to scavenge excess ROS from diseased cells.

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Degradation of ciprofloxacin by green cobalt oxide quantum dots

Bronzato

Bronzato²,

Brito³

et al. 2023

Applied Surface Science

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Nanostructured Hematite Decorated with Gold Nanoparticles for Functionalization and Biocompatibility

Cited by 4 publications

References 30 publications

Synthesis of bioluminescent gold nanoparticle–luciferase hybrid systems for technological applications

Synthesis of bioluminescent gold nanoparticle–luciferase hybrid systems for technological applications

Versatile Gold-Coupled Te-Carbon Dots for Quantitative Monitoring and Efficient Scavenging of Superoxide Anions

Degradation of ciprofloxacin by green cobalt oxide quantum dots

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